ZFIN ID: ZDB-PUB-180207-9
Wilms Tumor 1b defines a wound-specific sheath cell subpopulation associated with notochord repair.
Lopez-Baez, J.C., Simpson, D.J., LLeras Forero, L., Zeng, Z., Brunsdon, H., Salzano, A., Brombin, A., Wyatt, C., Rybski, W., Huitema, L.F.A., Dale, R.M., Kawakami, K., Englert, C., Chandra, T., Schulte-Merker, S., Hastie, N.D., Patton, E.E.
Date: 2018
Source: eLIFE   7: (Journal)
Registered Authors: Brombin, Alessandro, Dale, Rodney M., Englert, Christoph, Hastie, Nicholas D., Huitema, Leonie, Kawakami, Koichi, Patton, E. Elizabeth, Schulte-Merker, Stefan, Wyatt, Cameron
Keywords: developmental biology, stem cells, zebrafish
MeSH Terms:
  • Animals
  • Cell Movement
  • Nerve Regeneration*
  • Neuroglia/chemistry*
  • Neuroglia/physiology*
  • Notochord/injuries*
  • WT1 Proteins/analysis*
  • Wound Healing*
  • Zebrafish
PubMed: 29405914 Full text @ Elife
Regenerative therapy for degenerative spine disorders requires the identification of cells that can slow down and possibly reverse degenerative processes. Here, we identify an unanticipated wound-specific notochord sheath cell subpopulation that expresses Wilms Tumor (WT) 1b following injury in zebrafish. We show that localized damage leads to Wt1b expression in sheath cells, and that wt1b+cells migrate into the wound to form a stopper-like structure, likely to maintain structural integrity. Wt1b+sheath cells are distinct in expressing cartilage and vacuolar genes, and in repressing a Wt1b-p53 transcriptional programme. At the wound, wt1b+and entpd5+ cells constitute separate, tightly-associated subpopulations. Surprisingly, wt1b expression at the site of injury is maintained even into adult stages in developing vertebrae, which form in an untypical manner via a cartilage intermediate. Given that notochord cells are retained in adult intervertebral discs, the identification of novel subpopulations may have important implications for regenerative spine disorder treatments.