header logo image header logo text
Downloads Login
General Information
ZFIN ID: ZDB-PERS-960805-291
Kawakami, Koichi
Email: kokawaka@lab.nig.ac.jp
Affiliation: K. Kawakami Lab
Address: Division of Molecular and Developmental Biology National Institute of Genetics 1111 Yata, Mishima Shizuoka, 411-8540 Japan
Country: Japan
Phone: 81-55-981-6740
Fax: 81-55-981-5827
Orcid ID:


Shiraki, T., Kawakami, K. (2018) A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish. Scientific Reports. 8:13366
Asakawa, K., Kawakami, K. (2018) Protocadherin-Mediated Cell Repulsion Controls the Central Topography and Efferent Projections of the Abducens Nucleus. Cell Reports. 24:1562-1572
Yang, Y.H.C., Kawakami, K., Stainier, D.Y. (2018) A new mode of pancreatic islet innervation revealed by live imaging in zebrafish. eLIFE. 7
Muto, A., Kawakami, K. (2018) Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae. Journal of visualized experiments : JoVE. (136)
Lal, P., Tanabe, H., Suster, M.L., Ailani, D., Kotani, Y., Muto, A., Itoh, M., Iwasaki, M., Wada, H., Yaksi, E., Kawakami, K. (2018) Identification of a neuronal population in the telencephalon essential for fear conditioning in zebrafish. BMC Biology. 16:45
Enya, S., Kawakami, K., Suzuki, Y., Kawaoka, S. (2018) A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor-liver crosstalk in causing adverse effects on the host.. Disease models & mechanisms. 11(8)
Iwasaki, M., Kuroda, J., Kawakami, K., Wada, H. (2018) Epidermal regulation of bone morphogenesis through the development and regeneration of osteoblasts in the zebrafish scale. Developmental Biology. 437(2):105-119
Lopez-Baez, J.C., Simpson, D.J., LLeras Forero, L., Zeng, Z., Brunsdon, H., Salzano, A., Brombin, A., Wyatt, C., Rybski, W., Huitema, L.F.A., Dale, R.M., Kawakami, K., Englert, C., Chandra, T., Schulte-Merker, S., Hastie, N.D., Patton, E.E. (2018) Wilms Tumor 1b defines a wound-specific sheath cell subpopulation associated with notochord repair.. eLIFE. 7
Leacock, S., Syed, P., James, V.M., Bode, A., Kawakami, K., Keramidas, A., Suster, M., Lynch, J.W., Harvey, R.J. (2018) Structure/Function Studies of the α4 Subunit Reveal Evolutionary Loss of a GlyR Subtype Involved in Startle and Escape Responses. Frontiers in molecular neuroscience. 11:23
Kawakami, K., Largaespada, D.A., Ivics, Z. (2017) Transposons As Tools for Functional Genomics in Vertebrate Models. Trends in genetics : TIG. 33(11):784-801
Matsuda, K., Yoshida, M., Kawakami, K., Hibi, M., Shimizu, T. (2017) Granule cells control recovery from classical conditioned fear responses in the zebrafish cerebellum. Scientific Reports. 7:11865
Muto, A., Lal, P., Ailani, D., Abe, G., Itoh, M., Kawakami, K. (2017) Activation of the hypothalamic feeding centre upon visual prey detection. Nature communications. 8:15029
Hasegawa, T., Hall, C.J., Crosier, P.S., Abe, G., Kawakami, K., Kudo, A., Kawakami, A. (2017) Transient inflammatory response mediated by interleukin-1β is required for proper regeneration in zebrafish fin fold. eLIFE. 6
Zhang, B.B., Yao, Y.Y., Zhang, H.F., Kawakami, K., Du, J.L. (2017) Left Habenula Mediates Light-Preference Behavior in Zebrafish via an Asymmetrical Visual Pathway. Neuron. 93(4):914-928.e4
Fukuda, R., Gunawan, F., Beisaw, A., Jimenez-Amilburu, V., Maischein, H.M., Kostin, S., Kawakami, K., Stainier, D.Y. (2017) Proteolysis regulates cardiomyocyte maturation and tissue integration. Nature communications. 8:14495
Reiten, I., Uslu, F.E., Fore, S., Pelgrims, R., Ringers, C., Diaz Verdugo, C., Hoffman, M., Lal, P., Kawakami, K., Pekkan, K., Yaksi, E., Jurisch-Yaksi, N. (2017) Motile-Cilia-Mediated Flow Improves Sensitivity and Temporal Resolution of Olfactory Computations. Current biology : CB. 27(2):166-174
Ohki, Y., Wenninger-Weinzierl, A., Hruscha, A., Asakawa, K., Kawakami, K., Haass, C., Edbauer, D., Schmid, B. (2017) Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration. Molecular neurodegeneration. 12:6
Heanue, T.A., Boesmans, W., Bell, D.M., Kawakami, K., Vanden Berghe, P., Pachnis, V. (2016) A Novel Zebrafish ret Heterozygous Model of Hirschsprung Disease Identifies a Functional Role for mapk10 as a Modifier of Enteric Nervous System Phenotype Severity. PLoS Genetics. 12:e1006439
Sternberg, J.R., Severi, K.E., Fidelin, K., Gomez, J., Ihara, H., Alcheikh, Y., Hubbard, J.M., Kawakami, K., Suster, M., Wyart, C. (2016) Optimization of a Neurotoxin to Investigate the Contribution of Excitatory Interneurons to Speed Modulation In Vivo. Current biology : CB. 26(17):2319-28
Zhang, R.W., Li, X.Q., Kawakami, K., Du, J.L. (2016) Stereotyped initiation of retinal waves by bipolar cells via presynaptic NMDA autoreceptors. Nature communications. 7:12650
Mizoguchi, T., Kawakami, K., Itoh, M. (2016) Zebrafish lines expressing UAS-driven red probes for monitoring cytoskeletal dynamics. Genesis (New York, N.Y. : 2000). 54(9):483-9
Shibata, E., Yokota, Y., Horita, N., Kudo, A., Abe, G., Kawakami, K., Kawakami, A. (2016) Fgf signalling controls diverse aspects of fin regeneration. Development (Cambridge, England). 143:2920-9
Kamezaki, A., Sato, F., Aoki, K., Asakawa, K., Kawakami, K., Matsuzaki, F., Sehara-Fujisawa, A. (2016) Visualization of Neuregulin 1 ectodomain shedding reveals its local processing in vitro and in vivo. Scientific Reports. 6:28873
Pipalia, T.G., Koth, J., Roy, S.D., Hammond, C.L., Kawakami, K., Hughes, S.M. (2016) Cellular dynamics of regeneration reveals role of two distinct Pax7 stem cell populations in larval zebrafish muscle repair. Disease models & mechanisms. 9(6):671-84
Böhm, U. L., Prendergast, A., Djenoune, L., Nunes Figueiredo, S., Gomez, J., Stokes, C., Kaiser, S., Suster, M., Kawakami, K., Charpentier, M., Concordet, J. P., Rio, J. P., Del Bene, F., Wyart, C. (2016) CSF-contacting neurons regulate locomotion by relaying mechanical stimuli to spinal circuits. Nature communications. 7:10866
Muto, A., Kawakami, K. (2016) Calcium Imaging of Neuronal Activity in Free-Swimming Larval Zebrafish. Methods in molecular biology (Clifton, N.J.). 1451:333-41
Tanabe, H., Seki, M., Itoh, M., Deepak, A., Lal, P., Horiuchi, T., Suzuki, Y., Kawakami, K. (2016) Fluorescence-Activated Cell Sorting and Gene Expression Profiling of GFP-Positive Cells from Transgenic Zebrafish Lines. Methods in molecular biology (Clifton, N.J.). 1451:93-106
Kawakami, K., Asakawa, K., Muto, A., Wada, H. (2016) Tol2-mediated transgenesis, gene trapping, enhancer trapping, and Gal4-UAS system. Methods in cell biology. 135:19-37
Kawakami, K., Asakawa, K., Hibi, M., Itoh, M., Muto, A., Wada, H. (2016) Gal4 Driver Transgenic Zebrafish: Powerful Tools to Study Developmental Biology, Organogenesis, and Neuroscience. Advances in genetics. 95:65-87
Yokota, Y., Nakajima, H., Wakayama, Y., Muto, A., Kawakami, K., Fukuhara, S., Mochizuki, N. (2015) Endothelial Ca(2+) oscillations reflect VEGFR signaling-regulated angiogenic capacity in vivo. eLIFE. 4
Kotani, Y., Morito, D., Yamazaki, S., Ogino, K., Kawakami, K., Takashima, S., Hirata, H., Nagata, K. (2015) Neuromuscular regulation in zebrafish by a large AAA+ ATPase/ubiquitin ligase, mysterin/RNF213. Scientific Reports. 5:16161
Rinkwitz, S., Geng, F., Manning, E., Suster, M., Kawakami, K., Becker, T.S. (2015) BAC transgenic zebrafish reveal hypothalamic enhancer activity around obesity associated SNP rs9939609 within the human FTO gene. Genesis (New York, N.Y. : 2000). 53(10):640-51
Koyanagi, M., Wada, S., Kawano-Yamashita, E., Hara, Y., Kuraku, S., Kosaka, S., Kawakami, K., Tamotsu, S., Tsukamoto, H., Shichida, Y., Terakita, A. (2015) Diversification of non-visual photopigment parapinopsin in spectral sensitivity for diverse pineal functions. BMC Biology. 13:73
Auer, T.O., Xiao, T., Bercier, V., Gebhardt, C., Duroure, K., Concordet, J.P., Wyart, C., Suster, M., Kawakami, K., Wittbrodt, J., Baier, H., Del Bene, F. (2015) Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3. eLIFE. 4
Xiao, Y., Faucherre, A., Pola-Morell, L., Heddleston, J.M., Liu, T.L., Chew, T.L., Sato, F., Sehara-Fujisawa, A., Kawakami, K., López-Schier, H. (2015) High-resolution live imaging reveals axon-glia interactions during peripheral nerve injury and repair in zebrafish. Disease models & mechanisms. 8:553-564
Sato, T., Sato, F., Kamezaki, A., Sakaguchi, K., Tanigome, R., Kawakami, K., Sehara-Fujisawa, A. (2015) Neuregulin 1 Type II-ErbB Signaling Promotes Cell Divisions Generating Neurons from Neural Progenitor Cells in the Developing Zebrafish Brain. PLoS One. 10:e0127360
Ogino, K., Low, S.E., Yamada, K., Saint-Amant, L., Zhou, W., Muto, A., Asakawa, K., Nakai, J., Kawakami, K., Kuwada, J.Y., Hirata, H. (2015) RING finger protein 121 facilitates the degradation and membrane localization of voltage-gated sodium channels. Proc. Natl. Acad. Sci. USA. 112(9):2859-64
Wai, H.A., Wada, H., Kawakami, K., Müller, F., Vernallis, A.B., Brown, G., Johnson, W.E. (2015) The development and growth of tissues derived from cranial neural crest and primitive mesoderm is dependent on the ligation status of retinoic acid receptor (RAR) γ: evidence that RARγ functions to maintain stem/progenitor cells in the absence of retinoic acid. Stem cells and development. 24(4):507-19
Wada, H., Kawakami, K. (2015) Size control during organogenesis: Development of the lateral line organs in zebrafish. Development, growth & differentiation. 57(2):169-78
Takeuchi, M., Matsuda, K., Yamaguchi, S., Asakawa, K., Miyasaka, N., Lal, P., Yoshihara, Y., Koga, A., Kawakami, K., Shimizu, T., Hibi, M. (2015) Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry. Developmental Biology. 397(1):1-17
Wada, H., Iwasaki, M., Kawakami, K. (2014) Development of the lateral line canal system through a bone remodeling process in zebrafish. Developmental Biology. 392(1):1-14
Okigawa, S., Mizoguchi, T., Okano, M., Tanaka, H., Isoda, M., Jiang, Y.J., Suster, M., Higashijima, S.I., Kawakami, K., Itoh, M. (2014) Different combinations of Notch ligands and receptors regulate V2 interneuron progenitor proliferation and V2a/V2b cell fate determination. Developmental Biology. 391(2):196-206
Nakayama, Y., Kikuta, H., Kanai, M., Yoshikawa, K., Kawamura, A., Kobayashi, K., Wang, Z., Khan, A., Kawakami, K., and Yamasu, K. (2013) Gbx2 functions as a transcriptional repressor to regulate the specification and morphogenesis of the mid-hindbrain junction in a dosage- and stage-dependent manner. Mechanisms of Development. 130(11-12):532-52
Sittaramane, V., Pan, X., Glasco, D.M., Huang, P., Gurung, S., Bock, A., Li, S., Wang, H., Kawakami, K., Matise, M.P., and Chandrasekhar, A. (2013) The PCP protein Vangl2 regulates migration of hindbrain motor neurons by acting in floor plate cells, and independently of cilia function. Developmental Biology. 382(2):400-412
Kwon, H.B., Fukuhara, S., Asakawa, K., Ando, K., Kashiwada, T., Kawakami, K., Hibi, M., Kwon, Y.G., Kim, K.W., Alitalo, K., and Mochizuki, N. (2013) The parallel growth of motoneuron axons with the dorsal aorta depends on Vegfc/Vegfr3 signaling in zebrafish. Development (Cambridge, England). 140(19):4081-4090
Satou, C., Kimura, Y., Hirata, H., Suster, M.L., Kawakami, K., and Higashijima, S. (2013) Transgenic tools to characterize neuronal properties of discrete populations of zebrafish neurons. Development (Cambridge, England). 140(18):3927-3931
Wada, H., Ghysen, A., Asakawa, K., Abe, G., Ishitani, T., and Kawakami, K. (2013) Wnt/Dkk Negative Feedback Regulates Sensory Organ Size in Zebrafish. Current biology : CB. 23(16):1559-65
Kishimoto, N., Asakawa, K., Madelaine, R., Blader, P., Kawakami, K., and Sawamoto, K. (2013) Interhemispheric asymmetry of olfactory input-dependent neuronal specification in the adult brain. Nature Neuroscience. 16(7):884-8
Muto, A., and Kawakami, K. (2013) Prey capture in zebrafish larvae serves as a model to study cognitive functions. Frontiers in neural circuits. 7:110
Asakawa, K., Abe, G., and Kawakami, K. (2013) Cellular dissection of the spinal cord motor column by BAC transgenesis and gene trapping in zebrafish. Frontiers in neural circuits. 7:100
Wada, H., Dambly-Chaudière, C., Kawakami, K., and Ghysen, A. (2013) Innervation is required for sense organ development in the lateral line system of adult zebrafish. Proc. Natl. Acad. Sci. USA. 110(14):5659-5664
Yamanaka, I., Miki, M., Asakawa, K., Kawakami, K., Oda, Y., and Hirata, H. (2013) Glycinergic transmission and postsynaptic activation of CaMKII are required for glycine receptor clustering in vivo. Genes to cells : devoted to molecular & cellular mechanisms. 18(3):211-224
Muto, A., Ohkura, M., Abe, G., Nakai, J., and Kawakami, K. (2013) Real-Time Visualization of Neuronal Activity during Perception. Current biology : CB. 23(4):307-311
Banjo, T., Grajcarek, J., Yoshino, D., Osada, H., Miyasaka, K.Y., Kida, Y.S., Ueki, Y., Nagayama, K., Kawakami, K., Matsumoto, T., Sato, M., and Ogura, T. (2013) Haemodynamically dependent valvulogenesis of zebrafish heart is mediated by flow-dependent expression of miR-21. Nature communications. 4:1978
Umeda, K., Shoji, W., Sakai, S., Muto, A., Kawakami, K., Ishizuka, T., and Yawo, H. (2013) Targeted expression of a chimeric channelrhodopsin in zebrafish under regulation of Gal4-UAS system. Neuroscience research. 75(1):69-75
Liu, W., Chen, J.R., Hsu, C.H., Li, Y.H., Chen, Y.M., Lin, C.Y., Huang, S.J., Chang, Z.K., Chen, Y.C., Lin, C.H., Gong, H.Y., Lin, C.C., Kawakami, K., and Wu, J.L. (2012) A zebrafish model of intrahepatic cholangiocarcinoma by dual expression of hepatitis B virus X and hepatitis C virus core protein in liver. Hepatology (Baltimore, Md.). 56(6):2268-2276
Shimizu, N., Kawakami, K., and Ishitani, T. (2012) Visualization and exploration of Tcf/Lef function using a highly responsive Wnt/beta-catenin signaling-reporter transgenic zebrafish. Developmental Biology. 370(1):71-85
Yano, T., Abe, G., Yokoyama, H., Kawakami, K., and Tamura, K. (2012) Mechanism of pectoral fin outgrowth in zebrafish development. Development (Cambridge, England). 139(16):2916-2925
Fukui, H., Shiba, D., Asakawa, K., Kawakami, K., and Yokoyama, T. (2012) The ciliary protein Nek8/Nphp9 acts downstream of Inv/Nphp2 during pronephros morphogenesis and left-right establishment in zebrafish. FEBS letters. 586(16):2273-2279
Pujol-Martí, J., Zecca, A., Baudoin, J.P., Faucherre, A., Asakawa, K., Kawakami, K., and Lopez-Schier, H. (2012) Neuronal birth order identifies a dimorphic sensorineural map. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(9):2976-2987
Asakawa, K., Higashijima, S.I., and Kawakami, K. (2012) An mnr2b/hlxb9lb enhancer trap line that labels spinal and abducens motor neurons in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 241(2):327-332
Tsetskhladze, Z.R., Canfield, V.A., Ang, K.C., Wentzel, S.M., Reid, K.P., Berg, A.S., Johnson, S.L., Kawakami, K., and Cheng, K.C. (2012) Functional assessment of human coding mutations affecting skin pigmentation using zebrafish. PLoS One. 7(10):e47398
Kishimoto, N., Alfaro-Cervello, C., Shimizu, K., Asakawa, K., Urasaki, A., Nonaka, S., Kawakami, K., Garcia-Verdugo, J.M., and Sawamoto, K. (2011) Migration of neuronal precursors from the telencephalic ventricular zone into the olfactory bulb in adult zebrafish. The Journal of comparative neurology. 519(17):3549-65
Suster, M.L., Abe, G., Schouw, A., and Kawakami, K. (2011) Transposon-mediated BAC transgenesis in zebrafish. Nature Protocols. 6(12):1998-2021
Mizoguchi, T., Togawa, S., Kawakami, K., and Itoh, M. (2011) Neuron and sensory epithelial cell fate is sequentially determined by notch signaling in zebrafish lateral line development. The Journal of neuroscience : the official journal of the Society for Neuroscience. 31(43):15522-15530
Muto, A., and Kawakami, K. (2011) Imaging functional neural circuits in zebrafish with a new GCaMP and the Gal4FF-UAS system. Communicative & integrative biology. 4(5):566-568
Shakes, L.A., Abe, G., Eltayeb, M.A., Wolf, H.M., Kawakami, K., and Chatterjee, P.K. (2011) Generating libraries of iTol2-end insertions at BAC ends using loxP and lox511 Tn10 transposons. BMC Genomics. 12(1):351
Ikenaga, T., Urban, J.M., Gebhart, N., Hatta, K., Kawakami, K., and Ono, F. (2011) Formation of the spinal network in zebrafish determined by domain-specific pax genes. The Journal of comparative neurology. 519(8):1562-1579
Vogalis, F., Shiraki, T., Kojima, D., Wada, Y., Nishiwaki, Y., Jarvinen, J.L., Sugiyama, J., Kawakami, K., Masai, I., Kawamura, S., Fukada, Y., and Lamb,T.D. (2011) Ectopic expression of cone-specific G-protein-coupled receptor kinase GRK7 in zebrafish rods leads to lower photosensitivity and altered responses. The Journal of physiology. 589(9):2321-2348
Muto, A., Ohkura, M., Kotani, T., Higashijima, S.I., Nakai, J., and Kawakami, K. (2011) Genetic visualization with an improved GCaMP calcium indicator reveals spatiotemporal activation of the spinal motor neurons in zebrafish. Proc. Natl. Acad. Sci. USA. 108(13):5425-5430
Hu, S.Y., Liao, C.H., Lin, Y.P., Li, Y.H., Gong, H.Y., Lin, G.H., Kawakami, K., Yang, T.H., and Wu, J.L. (2011) Zebrafish eggs used as bioreactors for the production of bioactive tilapia insulin-like growth factors. Transgenic Research. 20(1):73-83
Tsujita, T., Li, L., Nakajima, H., Iwamoto, N., Nakajima-Takagi, Y., Ohashi, K., Kawakami, K., Kumagai, Y., Freeman, B.A., Yamamoto, M., and Kobayashi, M. (2011) Nitro-fatty acids and cyclopentenone prostaglandins share strategies to activate the Keap1-Nrf2 system: a study using green fluorescent protein transgenic zebrafish. Genes to cells : devoted to molecular & cellular mechanisms. 16(1):46-57
Abe, G., Suster, M.L., and Kawakami, K. (2011) Tol2-mediated Transgenesis, Gene Trapping, Enhancer Trapping, and the Gal4-UAS System. Methods in cell biology. 104:23-49
Agetsuma, M., Aizawa, H., Aoki, T., Nakayama, R., Takahoko, M., Goto, M., Sassa, T., Amo, R., Shiraki, T., Kawakami, K., Hosoya, T., Higashijima, S.I., and Okamoto, H. (2010) The habenula is crucial for experience-dependent modification of fear responses in zebrafish. Nature Neuroscience. 13(11):1354-1356
Kawakami, K., Abe, G., Asada, T., Asakawa, K., Fukuda, R., Ito, A., Lal, P., Mouri, N., Muto, A., Suster, M.L., Takakubo, H., Urasaki, A., Wada, H., and Yoshida, M. (2010) zTrap: zebrafish gene trap and enhancer trap database. BMC Developmental Biology. 10:105
Asakawa, K., and Kawakami, K. (2010) A transgenic zebrafish for monitoring in vivo microtubule structures. Developmental dynamics : an official publication of the American Association of Anatomists. 239(10):2695-2699
Imai, F., Yoshizawa, A., Fujimori-Tonou, N., Kawakami, K., and Masai, I. (2010) The ubiquitin proteasome system is required for cell proliferation of the lens epithelium and for differentiation of lens fiber cells in zebrafish. Development (Cambridge, England). 137(19):3257-3268
Hu, S.Y., Lin, P.Y., Liao, C.H., Gong, H.Y., Lin, G.H., Kawakami, K., and Wu, J.L. (2010) Nitroreductase-mediated Gonadal Dysgenesis for Infertility Control of Genetically Modified Zebrafish. Marine biotechnology (New York, N.Y.). 12(5):569-578
Bussmann, J., Bos, F.L., Urasaki, A., Kawakami, K., Duckers, H.J., and Schulte-Merker, S. (2010) Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk. Development (Cambridge, England). 137(16):2653-2657
Yamamoto, M., Morita, R., Mizoguchi, T., Matsuo, H., Isoda, M., Ishitani, T., Chitnis, A.B., Matsumoto, K., Crump, J.G., Hozumi, K., Yonemura, S., Kawakami, K., and Itoh, M. (2010) Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions. Development (Cambridge, England). 137(15):2527-2537
Chen, Y.C., Wu, B.K., Chu, C.Y., Cheng, C.H., Han, H.W., Chen, G.D., Lee, M.T., Hwang, P.P., Kawakami, K., Chang, C.C., and Huang, C.J. (2010) Identification and characterization of alternative promoters of zebrafish Rtn-4/Nogo genes in cultured cells and zebrafish embryos. Nucleic acids research. 38(14):4635-4650
Rodriguez-Mari, A., Cañestro, C., Bremiller, R.A., Nguyen-Johnson, A., Asakawa, K., Kawakami, K., and Postlethwait, J.H. (2010) Sex Reversal in Zebrafish fancl Mutants Is Caused by Tp53-Mediated Germ Cell Apoptosis. PLoS Genetics. 6(7):e1001034
Pujol-Martí, J., Baudoin, J.P., Faucherre, A., Kawakami, K., and Lopez-Schier, H. (2010) Progressive neurogenesis defines lateralis somatotopy. Developmental dynamics : an official publication of the American Association of Anatomists. 239(7):1919-1930
Sinha, D.K., Neveu, P., Gagey, N., Aujard, I., Le Saux, T., Rampon, C., Gauron, C., Kawakami, K., Leucht, C., Bally-Cuif, L., Volovitch, M., Bensimon, D., Jullien, L., and Vriz, S. (2010) Photoactivation of the CreER(T2) Recombinase for Conditional Site-Specific Recombination with High Spatiotemporal Resolution. Zebrafish. 7(2):199-204
Wada, H., Ghysen, A., Satou, C., Higashijima, S.I., Kawakami, K., Hamaguchi, S., and Sakaizumi, M. (2010) Dermal morphogenesis controls lateral line patterning during postembryonic development of teleost fish. Developmental Biology. 340(2):583-594
Kotani, T., Iemura, S.I., Natsume, T., Kawakami, K., and Yamashita, M. (2010) Mys protein regulates protein kinase A activity by interacting with regulatory type I{alpha} subunit during vertebrate development. The Journal of biological chemistry. 285(7):5106-5116
Takeuchi, M., Kaneko, H., Nishikawa, K., Kawakami, K., Yamamoto, M., and Kobayashi, M. (2010) Efficient transient rescue of hematopoietic mutant phenotypes in zebrafish using Tol2-mediated transgenesis. Development, growth & differentiation. 52(2):245-250
Kajita, M., Hogan, C., Harris, A.R., Dupre-Crochet, S., Itasaki, N., Kawakami, K., Charras, G., Tada, M., and Fujita, Y. (2010) Interaction with surrounding normal epithelial cells influences signalling pathways and behaviour of Src-transformed cells. Journal of Cell Science. 123(Pt 2):171-180
Appelbaum, L., Wang, G.X., Maro, G.S., Mori, R., Tovin, A., Marin, W., Yokogawa, T., Kawakami, K., Smith, S.J., Gothilf, Y., Mignot, E., and Mourrain, P. (2009) Sleep-wake regulation and hypocretin-melatonin interaction in zebrafish. Proc. Natl. Acad. Sci. USA. 106(51):21942-21947
Komisarczuk, A.Z., Kawakami, K., and Becker, T.S. (2009) Cis-regulation and chromosomal rearrangement of the fgf8 locus after the Teleost/tetrapod split. Developmental Biology. 336(2):301-312
Sugiyama, M., Sakaue-Sawano, A., Iimura, T., Fukami, K., Kitaguchi, T., Kawakami, K., Okamoto, H., Higashijima, S.I., and Miyawaki, A. (2009) Illuminating cell-cycle progression in the developing zebrafish embryo. Proc. Natl. Acad. Sci. USA. 106(49):20812-20817
Asakawa, K., and Kawakami, K. (2009) The Tol2-mediated Gal4-UAS method for gene and enhancer trapping in zebrafish. Methods (San Diego, Calif.). 49(3):275-281
Suster, M.L., Sumiyama, K., and Kawakami, K. (2009) Transposon-mediated BAC transgenesis in zebrafish and mice. BMC Genomics. 10:477
Kim, D.J., Seok, S.H., Baek, M.W., Lee, H.Y., Na, Y.R., Park, S.H., Lee, H.K., Dutta, N.K., Kawakami, K., and Park, J.H. (2009) Estrogen-responsive transient expression assay using a brain aromatase-based reporter gene in zebrafish (Danio rerio). Comparative medicine. 59(5):416-423
Picker, A., Cavodeassi, F., Machate, A., Bernauer, S., Hans, S., Abe, G., Kawakami, K., Wilson, S.W., and Brand, M. (2009) Dynamic coupling of pattern formation and morphogenesis in the developing vertebrate retina. PLoS Biology. 7(10):e1000214
Koide, T., Miyasaka, N., Morimoto, K., Asakawa, K., Urasaki, A., Kawakami, K., and Yoshihara, Y. (2009) Olfactory neural circuitry for attraction to amino acids revealed by transposon-mediated gene trap approach in zebrafish. Proc. Natl. Acad. Sci. USA. 106(24):9884-9889
Kitaguchi, T., Kawakami, K., and Kawahara, A. (2009) Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis. Mechanisms of Development. 126(5-6):314-323
Kim, D.J., Seok, S.H., Baek, M.W., Lee, H.Y., Na, Y.R., Park, S.H., Lee, H.K., Dutta, N.K., Kawakami, K., and Park, J.H. (2009) Benomyl induction of brain aromatase and toxic effects in the zebrafish embryo. Journal of applied toxicology : JAT. 29(4):289-294
Esaki, M., Hoshijima, K., Nakamura, N., Munakata, K., Tanaka, M., Ookata, K., Asakawa, K., Kawakami, K., Wang, W., Weinberg, E.S., and Hirose, S. (2009) Mechanism of development of ionocytes rich in vacuolar-type H(+)-ATPase in the skin of zebrafish larvae. Developmental Biology. 329(1):116-129
Chen, Y.C., Cheng, C.H., Chen, G.D., Hung, C.C., Yang, C.H., Hwang, S.P., Kawakami, K., Wu, B.K., and Huang, C.J. (2009) Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene. Developmental dynamics : an official publication of the American Association of Anatomists. 238(3):746-754
Kim, D.J., Seok, S.H., Baek, M.W., Lee, H.Y., Na, Y.R., Park, S.H., Lee, H.K., Dutta, N.K., Kawakami, K., and Park, J.H. (2009) Developmental toxicity and brain aromatase induction by high genistein concentrations in zebrafish embryos. Toxicology mechanisms and methods. 19(3):251-256
Suster, M.L., Kania, A., Liao, M., Asakawa, K., Charron, F., Kawakami, K., and Drapeau, P. (2009) A novel conserved evx1 enhancer links spinal interneuron morphology and cis-regulation from fish to mammals. Developmental Biology. 325(2):422-433
Santoriello, C., Deflorian, G., Pezzimenti, F., Kawakami, K., Lanfrancone, L., d'Adda di Fagagna, F., and Mione, M. (2009) Expression of H-RASV12 in a zebrafish model of Costello syndrome causes cellular senescence in adult proliferating cells. Disease models & mechanisms. 2(1-2):56-67
Suster, M.L., Kikuta, H., Urasaki, A., Asakawa, K., and Kawakami, K. (2009) Transgenesis in zebrafish with the tol2 transposon system. Methods in molecular biology (Clifton, N.J.). 561:41-63
Urasaki, A., and Kawakami, K. (2009) Analysis of genes and genome by the tol2-mediated gene and enhancer trap methods. Methods in molecular biology (Clifton, N.J.). 546:85-102
Kikuta, H., and Kawakami, K. (2009) Transient and stable transgenesis using tol2 transposon vectors. Methods in molecular biology (Clifton, N.J.). 546:69-84
Faucherre, A., Pujol-Martí, J., Kawakami, K., and Lopez-Schier, H. (2009) Afferent neurons of the zebrafish lateral line are strict selectors of hair-cell orientation. PLoS One. 4(2):e4477
Urasaki, A., Asakawa, K., and Kawakami, K. (2008) Efficient transposition of the Tol2 transposable element from a single-copy donor in zebrafish. Proc. Natl. Acad. Sci. USA. 105(50):19827-19832
Yoshikawa, S., Kawakami, K., and Zhao, X.C. (2008) G2R Cre reporter transgenic zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 237(9):2460-2465
Asakawa, K., and Kawakami, K. (2008) Targeted gene expression by the Gal4-UAS system in zebrafish. Development, growth & differentiation. 50(6):391-399
Kotani, T., and Kawakami, K. (2008) misty somites, a maternal effect gene identified by transposon-mediated insertional mutagenesis in zebrafish that is essential for the somite boundary maintenance. Developmental Biology. 316(2):383-396
Asakawa, K., Suster, M.L., Mizusawa, K., Nagayoshi, S., Kotani, T., Urasaki, A., Kishimoto, Y., Hibi, M., and Kawakami, K. (2008) Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish. Proc. Natl. Acad. Sci. USA. 105(4):1255-1260
Nagayoshi, S., Hayashi, E., Abe, G., Osato, N., Asakawa, K., Urasaki, A., Horikawa, K., Ikeo, K., Takeda, H., and Kawakami, K. (2008) Insertional mutagenesis by the Tol2 transposon-mediated enhancer trap approach generated mutations in two developmental genes: tcf7 and synembryn-like. Development (Cambridge, England). 135(1):159-169
Aleström, P., Begemann, G., Carvan, M.J. 3rd, Cheng, K.C., Crosier, K., Crosier, P., Ekker, S., Huttenlocher, A., Kawakami, K., Kelly, G., Korzh, V., Lieschke, G., Mione, M., Neely, M.N., Neuhauss, S., and Trede, N.S. (2008) Views on four key questions about zebrafish research. Zebrafish. 5(1):9-24
Fan, X., Hagos, E.G., Xu, B., Sias, C., Kawakami, K., Burdine, R.D., and Dougan, S.T. (2007) Nodal signals mediate interactions between the extra-embryonic and embryonic tissues in zebrafish. Developmental Biology. 310(2):363-378
Seguchi, O., Takashima, S., Yamazaki, S., Asakura, M., Asano, Y., Shintani, Y., Wakeno, M., Minamino, T., Kondo, H., Furukawa, H., Nakamaru, K., Naito, A., Takahashi, T., Ohtsuka, T., Kawakami, K., Isomura, T., Kitamura, S., Tomoike, H., Mochizuki, N., and Kitakaze, M. (2007) A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart. J. Clin. Invest.. 117(10):2812-2824
Nakada, T., Hoshijima, K., Esaki, M., Nagayoshi, S., Kawakami, K., and Hirose, S. (2007) Localization of ammonia transporter Rhcg1 in mitochondrion-rich cells of yolk sac, gill, and kidney of zebrafish and its ionic strength-dependent expression. American journal of physiology. Regulatory, integrative and comparative physiology. 293(4):R1743-1753
Scott, E.K., Mason, L., Arrenberg, A.B., Ziv, L., Gosse, N.J., Xiao, T., Chi, N.C., Asakawa, K., Kawakami, K., and Baier, H. (2007) Targeting neural circuitry in zebrafish using GAL4 enhancer trapping. Nature Methods. 4(4):323-326
Kosaka, K., Kawakami, K., Sakamoto, H., and Inoue, K. (2007) Spatiotemporal localization of germ plasm RNAs during zebrafish oogenesis. Mechanisms of Development. 124(4):279-289
Kawakami, K. (2007) Tol2: a versatile gene transfer vector in vertebrates. Genome biology. 8(1):S7
Jeong, J.Y., Einhorn, Z., Mathur, P., Chen, L., Lee, S., Kawakami, K., and Guo, S. (2007) Patterning the zebrafish diencephalon by the conserved zinc-finger protein Fezl. Development (Cambridge, England). 134(1):127-136
Esaki, M., Hoshijima, K., Kobayashi, S., Fukuda, H., Kawakami, K., and Hirose, S. (2007) Visualization in zebrafish larvae of Na+ uptake in mitochondrion-rich cells whose differentiation is dependent on foxi3{alpha}. American journal of physiology. Regulatory, integrative and comparative physiology. 292(1):R470-R480
Zou, J., Beermann, F., Wang, J., Kawakami, K., and Wei, X. (2006) The Fugu tyrp1 promoter directs specific GFP expression in zebrafish: tools to study the RPE and the neural crest-derived melanophores. Pigment cell research. 19(6):615-627
Blaser, H., Reichman-Fried, M., Castanon, I., Dumstrei, K., Marlow, F.L., Kawakami, K., Solnica-Krezel, L., Heisenberg, C.P., and Raz, E. (2006) Migration of zebrafish primordial germ cells: a role for Myosin contraction and cytoplasmic flow. Developmental Cell. 11(5):613-627
Urasaki, A., Morvan, G., and Kawakami, K. (2006) Functional dissection of the Tol2 transposable element identified the minimal cis-sequence and a highly repetitive sequence in the subterminal region essential for transposition. Genetics. 174(2):639-649
Inoue, F., Nagayoshi, S., Ota, S., Islam, M.E., Tonou-Fujimori, N., Odaira, Y., Kawakami, K., and Yamasu, K. (2006) Genomic organization, alternative splicing, and multiple regulatory regions of the zebrafish fgf8 gene. Development, growth & differentiation. 48(7):447-462
Kotani, T., Nagayoshi, S., Urasaki, A., and Kawakami, K. (2006) Transposon-mediated gene trapping in zebrafish. Methods (San Diego, Calif.). 39(3):199-206
Fisher, S., Grice, E.A., Vinton, R.M., Bessling, S.L., Urasaki, A., Kawakami, K.,and McCallion, A.S. (2006) Evaluating the biological relevance of putative enhancers using Tol2 transposon-mediated transgenesis in zebrafish. Nature Protocols. 1(3):1297-1305
Kawakami, K. (2005) Transposon tools and methods in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 234(2):244-254
Taylor, M.R., Kikkawa, S., Diez-Juan, A., Ramamurthy, V., Kawakami, K., Carmeliet, P., and Brockerhoff, S.E. (2005) The Zebrafish pob Gene Encodes a Novel Protein Required for Survival of Red Cone Photoreceptor Cells. Genetics. 170(1):263-273
Kawakami, K., Takeda, H., Kawakami, N., Kobayashi, M., Matsuda, N., and Mishina, M. (2004) A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish. Developmental Cell. 7(1):133-144
Kawakami, K. (2004) Transgenesis and gene trap methods in zebrafish by using the Tol2 transposable element. The Zebrafish: Genetics, Genomics and Informatics, 2nd ed., Methods Cell Biol.. 77:201-222
Kawakami, K. (2001) Functional analysis of zebrafish genes by insertional mutagenesis. Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme. (Suppl. 16) 46:2456-2460
Kawakami, K. (2000) [Insertional mutagenesis in zebrafish and a proviral insertional mutation hagoromo].. Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme. 45(Suppl. 17):2810-2819
Kawakami, K., Shima, A., and Kawakami, N. (2000) Identification of a functional transposase of the Tol2 element, an Ac-like element from the Japanese medaka fish, and its transposition in the zebrafish germ lineage. Proc. Natl. Acad. Sci. USA. 97(21):11403-11408
Kawakami, K., Amsterdam, A., Shimoda, N., Becker, T., Mugg, J., Shima, A., and Hopkins, N. (2000) Proviral insertions in the zebrafish hagoromo gene, encoding an F-box/WD40-repeat protein, cause stripe pattern anomalies. Current biology : CB. 10(8):463-466
Kawakami, K. and Shima A. (1999) Identification of the Tol2 transposase of the medaka fish Oryzias latipes that catalyzes excision of a nonautonomous Tol2 element in zebrafish Danio rerio. Gene. 240(1):239-244
Kawakami, K., Koga, A., Hori, H., and Shima, A. (1998) Excision of the Tol2 transposable element of the medaka fish, Oryzias latipes, in zebrafish, Danio rerio. Gene. 225:17-22
Yoon, C., Kawakami, K., and N. Hopkins (1997) Zebrafish vasa homologue RNA is localized to the cleavage planes of 2- and 4-cell-stage embryos and is expressed in the primordial germ cells. Development (Cambridge, England). 124(16):3157-3165
Amsterdam, A., Yoon, C., Allende, M., Becker, T., Kawakami, K., Burgess, S., Gaiano, N., and Hopkins, N. (1997) Retrovirus-mediated insertional mutagenesis in zebrafish and identification of a molecular marker for embryonic germ cells. Cold Spring Harbor symposia on quantitative biology. 62:437-450
Allende, M.L., Amsterdam, A., Becker, T., Kawakami, K., Gaiano, N., and Hopkins, N. (1996) Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development. Genes & Development. 10(24):3141-3155
Gaiano, N., Amsterdam, A., Kawakami, K., Allende, M., Becker, T., and Hopkins, N. (1996) Insertional mutagenesis and rapid cloning of essential genes in zebrafish. Nature. 383(6603):829-832
Gaiano, N., Allende, M., Amsterdam, A., Kawakami, K., and Hopkins, N. (1996) Highly efficient germ-line transmission of proviral insertions in zebrafish. Proc. Natl. Acad. Sci. USA. 93(15):7777-7782
Kawakami, K. and Hopkins, N. (1996) Rapid identification of transgenic zebrafish. Trends in genetics : TIG. 12:9-10

Kawakami, K. and Noda, T. (2004) Transposition of the Tol2 element, an Ac-like element from the Japanese medaka fish Oryzias latipes, in mouse embryonic stem cells. Genetics 166:895-899.

Kawakami, K., Imanaka, K., Itoh, M., and Taira, M. (2004) Excision of the Tol2 transposable element of the medaka fish Oryzias latipes in Xenopus laevis and Xenopus tropicalis. Gene 338:93-98.

The MGC project team (Gerhard, D.S. and 113 authors). (2004) The status, quality, and expansion of the NIH full-length cDNA project: The mammalian gene collection (MGC). Genome Res 14:2121-2127.

Terai, Y., Morikawa, N., Kawakami, K., and Okada, N. (2003) The complexity of alternative splicing of hagoromo mRNAs is increased in an explosively speciated lineage in East African cichlids. Proc. Natl. Acad. Sci. USA 100:12798-12803.

Clark, M.S., Edwards, Y.J.K., Peterson, D., Clifton, S.W., Thompson, A.J., Sasaki, M., Suzuki, Y., Kikuchi, K., Watabe, S., Kawakami, K., Sugano. S., Elgar, G., and Johnson, S.L. (2003) Fugu ESTs: New resources for transcription analysis and genome annotation. Genome Research 13:2747-2753.

Terai, Y., Morikawa, N., Kawakami, K., and Okada, N. (2002) Accelerated evolution of the surface amino acids in the WD-repeat domain encoded by the hagoromo gene in an explosively speciated lineage of east cichlid fishes. Molecular Biology and Evolution 19:574-578.

Maruyama, S., Hatakeyama, S., Nakayama, K., Ishida, N., Kawakami, K., and Nakayama, K.-i. (2001) Characterization of a mouse gene (Fbxw6) that encodes a homologue of Caenorhabditis elegans SEL-10. Genomics 78:214-222.

Kawakami, K., Pande, S., Faiola, B., Moore, D. P., Boeke, J. D., Farabaugh, P. J., Strathern, J. N., Nakamura, Y., and Garfinkel, D. J. (1993) A rare tRNA-Arg(CUU) that regulates Ty1 element ribosomal frameshifting is essential for Ty1 retrotransposition in Saccharomyces cerevisiae. Genetics 135:309-320.

Shirayama, M., Kawakami, K., Matsui, Y., Tanaka, K., and Toh-e, A. (1993) MSI3, a multicopy suppressor of mutants hyper-activated in the RAS-cAMP pathway, encodes a novel HSP70 protein of Saccharomyces cerevisiae. Mol. Gen. Genet. 240:323-332.

Ito, K., Kawakami, K., and Nakamura, Y. (1993) Multiple control of Escherichia coli lysyl-tRNA synthetase expression involves a transcriptional repressor and a translational enhancer element. Proc. Natl. Acad. Sci. USA 90:302-306.

Kawakami, K., Ito, K., and Nakamura, Y. (1992) Differential regulation of two genes encoding lysyl-tRNA synthetases in Escherichia coli: lysU-constitutive mutations compensate for a lysS null mutation. Molecular Microbiology 6:1739-1746.

Nakamura, Y., and Kawakami, K. (1992) Overproduction and purification of lysyl-tRNA synthetase encoded by the herC gene of Escherichia coli. Biochimie 74:581-584.

Kawakami, K., Shafer, B. K., Garfinkel, D. J., Strathern, J. N., and Nakamura, Y. (1992) Ty element-induced temperature sensitive mutations of Saccharomyces cerevisiae. Genetics 131:821-832.

Mikuni, O., Kawakami, K., and Nakamura, Y. (1991) Sequence and functional analysis of mutations in the gene encoding peptide-chain-release factor 2 of Escherichia coli. Biochimie 73:1509-1516.

Nakamura, Y., Kawakami, K., and Mikuni, O. (1990) Alternative translation and functional diversity of release factor 2 and lysyl-tRNA synthetase. In Post-transcriptional control of gene expression: Springer-Verlag, pp. 455-464.

Kawakami, K., and Nakamura, Y. (1990) Autogenous suppression of an opal mutation in the gene encoding peptide chain release factor 2. Proc. Natl. Acad. Sci. USA 87:8432-8436.

Kawakami, K., Naito, S., Inoue, N., Nakamura, Y., Ikeda, H., and Uchida, H. (1989) Isolation and characterization of herC, a mutation of Escherichia coli affecting maintenance of ColE1. Mol. Gen. Genet. 219:333-340.

Inada, T., Kawakami, K., Chen, S.-M., Takiff, H., Court, D. L., and Nakamura, Y. (1989) Temperature-sensitive lethal mutant of Era, a G protein in Escherichia coli. Journal of Bacteriology 171:5017-5024.

Kawakami, K., Inada, T., and Nakamura, Y. (1988) Conditionally lethal and recessive UGA-suppressor mutations in the prfB gene encoding peptide chain release factor 2 of Escherichia coli. Journal of Bacteriology 170:5378-5381.

Kawakami, K., Josson, Y. H., Bjork, G. R., Ikeda, H., and Nakamura, Y. (1988) Chromosomal location and structure of the operon encoding peptide-chain-release factor 2 of Escherichia coli. Proc. Natl. Acad. Sci. USA 85:5620-5624.