PUBLICATION

Pannexin 1 Is Critically Involved in Feedback from Horizontal Cells to Cones

Authors
Cenedese, V., de Graaff, W., Csikós, T., Poovayya, M., Zoidl, G., Kamermans, M.
ID
ZDB-PUB-180130-1
Date
2017
Source
Frontiers in molecular neuroscience   10: 403 (Journal)
Registered Authors
Cenedese, Valentina, Kamermans, Maarten, Zoidl, Georg
Keywords
Pannexin 1, cones, connexins, feedback, horizontal cells, lateral inhibition, retina, zebrafish
MeSH Terms
none
PubMed
29375296 Full text @ Front. Mol. Neurosci.
Abstract
Retinal horizontal cells (HCs) feed back negatively to cone photoreceptors and in that way generate the center/surround organization of bipolar cell receptive fields. The mechanism by which HCs inhibit photoreceptors is a matter of debate. General consensus exists that horizontal cell activity leads to the modulation of the cone Ca-current. This modulation has two components, one fast and the other slow. Several mechanisms for this modulation have been proposed: a fast ephaptic mechanism, and a slow pH mediated mechanism. Here we test the hypothesis that the slow negative feedback signal from HCs to cones is mediated by Panx1 channels expressed at the tips of the dendrites of horizontal cell. We generated zebrafish lacking Panx1 and found that the slow component of the feedback signal was strongly reduced in the mutants showing that Panx1 channels are a fundamental part of the negative feedback pathway from HCs to cones.
Genes / Markers
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Expression
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Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hu1795
    		Point Mutation
    nhi6
      		Small Deletion
      nhi7
        		Small Deletion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        panx1aTALEN1-panx1aTALEN
        panx1bTALEN1-panx1bTALEN
        1 - 2 of 2
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        Fish
        Fish
        gja9ahu1795/hu1795; panx1anhi6/nhi6
        panx1anhi6/nhi6
        panx1anhi6/nhi6; panx1bnhi7/nhi7
        panx1bnhi7/nhi7
        TL
        1 - 5 of 5
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        Orthology
        No data available
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        No data available
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        Citation
        Cenedese, V., de Graaff, W., Csikós, T., Poovayya, M., Zoidl, G., Kamermans, M. (2017) Pannexin 1 Is Critically Involved in Feedback from Horizontal Cells to Cones. Frontiers in molecular neuroscience. 10:403.