PUBLICATION
Remodeling of Mitochondrial Flashes in Muscular Development and Dystrophy in Zebrafish
- Authors
- Zhang, M., Sun, T., Jian, C., Lei, L., Han, P., Lv, Q., Yang, R., Zhou, X., Xu, J., Hu, Y., Men, Y., Huang, Y., Zhang, C., Zhu, X., Wang, X., Cheng, H., Xiong, J.W.
- ID
- ZDB-PUB-150718-1
- Date
- 2015
- Source
- PLoS One 10: e0132567 (Journal)
- Registered Authors
- Han, Peidong, Xiong, Jing-Wei
- Keywords
- Mitochondria, Skeletal muscles, Embryos, Zebrafish, Skeletal muscle fibers, Dystrophin, Muscle fibers, Muscle cells
- MeSH Terms
-
- Recombinant Fusion Proteins/genetics
- Recombinant Fusion Proteins/metabolism
- Time-Lapse Imaging
- Animals, Genetically Modified
- NADP/metabolism
- Superoxide Dismutase/genetics
- Superoxide Dismutase/metabolism
- Disease Models, Animal
- Mitochondrial Dynamics/genetics*
- Zebrafish
- Bacterial Proteins/genetics
- Bacterial Proteins/metabolism
- Signal Transduction
- Muscle Development/genetics
- Membrane Proteins/antagonists & inhibitors
- Membrane Proteins/genetics*
- Membrane Proteins/metabolism
- Actins/genetics
- Actins/metabolism
- Mitochondria/metabolism*
- Mitochondria/pathology
- Muscle Proteins/antagonists & inhibitors
- Muscle Proteins/genetics*
- Muscle Proteins/metabolism
- Zebrafish Proteins/antagonists & inhibitors
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Animals
- Muscular Dystrophies/genetics*
- Muscular Dystrophies/metabolism
- Muscular Dystrophies/pathology
- Genes, Reporter
- Muscle, Skeletal/metabolism*
- Muscle, Skeletal/pathology
- Luminescent Proteins/genetics
- Luminescent Proteins/metabolism
- Gene Expression Regulation, Developmental
- Embryo, Nonmammalian
- Morpholinos/genetics
- Morpholinos/metabolism
- PubMed
- 26186000 Full text @ PLoS One
Citation
Zhang, M., Sun, T., Jian, C., Lei, L., Han, P., Lv, Q., Yang, R., Zhou, X., Xu, J., Hu, Y., Men, Y., Huang, Y., Zhang, C., Zhu, X., Wang, X., Cheng, H., Xiong, J.W. (2015) Remodeling of Mitochondrial Flashes in Muscular Development and Dystrophy in Zebrafish. PLoS One. 10:e0132567.
Abstract
Mitochondrial flash (mitoflash) is a highly-conserved, universal, and physiological mitochondrial activity in isolated mitochondria, intact cells, and live organisms. Here we investigated developmental and disease-related remodeling of mitoflash activity in zebrafish skeletal muscles. In transgenic zebrafish expressing the mitoflash reporter cpYFP, in vivo imaging revealed that mitoflash frequency and unitary properties underwent multiphasic and muscle type-specific changes, accompanying mitochondrial morphogenesis from 2 to 14 dpf. In particular, short (S)-type mitoflashes predominated in early muscle formation, then S-, transitory (T)- and regular (R)-type mitoflashes coexisted during muscle maturation, followed by a switch to R-type mitoflashes in mature skeletal muscles. In early development of muscular dystrophy, we found accelerated S- to R-type mitoflash transition and reduced mitochondrial NAD(P)H amidst a remarkable cell-to-cell heterogeneity. This study not only unravels a profound functional and morphological remodeling of mitochondria in developing and diseased skeletal muscles, but also underscores mitoflashes as a useful reporter of mitochondrial function in milieu of live animals under physiological and pathophysiological conditions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping