ZFIN Person: Wittbrodt, Jochen

ZFIN ID: ZDB-PERS-960805-615

ZFIN ID: ZDB-PERS-960805-615

Wittbrodt, Jochen

Email:	jochen.wittbrodt@zoo.uni-heidelberg.de
URL:
Affiliation:	Jochen Wittbrodt Lab SFB 271 Junior Group
Address:	University of Heidelberg and Karlsruhe Institute of Technology, KIT Heidelberg Institute for Zoology Im Neuenheimer Feld 230 Heidelberg, D-69120 Germany
Country:	Germany
Phone:	+49 6221 546497
Fax:	+49 6221 545639
ORCID ID:

BIOGRAPHY AND RESEARCH INTERESTS

PUBLICATIONS

Cornean, A., Gierten, J., Welz, B., Mateo, J.L., Thumberger, T., Wittbrodt, J. (2022) Precise in vivo functional analysis of DNA variants with base editing using ACEofBASEs target prediction. eLIFE. 11:

Thumberger, T., Tavhelidse-Suck, T., Gutierrez-Triana, J.A., Cornean, A., Medert, R., Welz, B., Freichel, M., Wittbrodt, J. (2022) Boosting targeted genome editing using the hei-tag. eLIFE. 11:

Beedgen, L., Hüllen, A., Gücüm, S., Thumberger, T., Wittbrodt, J., Thiel, C. (2021) A rapid and simple procedure for the isolation and cultivation of fibroblast-like cells from medaka and zebrafish embryos and fin clip biopsies. Laboratory animals. 56(3):270-278

Zilova, L., Weinhardt, V., Tavhelidse, T., Schlagheck, C., Thumberger, T., Wittbrodt, J. (2021) Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development. eLIFE. 10:

Wagner, N., Beuttenmueller, F., Norlin, N., Gierten, J., Boffi, J.C., Wittbrodt, J., Weigert, M., Hufnagel, L., Prevedel, R., Kreshuk, A. (2021) Deep learning-enhanced light-field imaging with continuous validation. Nature Methods. 18:557-563

Bosze, B., Ono, Y., Mattes, B., Sinner, C., Gourain, V., Thumberger, T., Tlili, S., Wittbrodt, J., Saunders, T.E., Strähle, U., Schug, A., Scholpp, S. (2020) Pcdh18a regulates endocytosis of E-cadherin during axial mesoderm development in zebrafish. Histochemistry and cell biology. 154(5):463-480

Lischik, C.Q., Adelmann, L., Wittbrodt, J. (2019) Enhanced in vivo-imaging in medaka by optimized anaesthesia, fluorescent protein selection and removal of pigmentation. PLoS One. 14:e0212956

Hammouda, O.T., Böttger, F., Wittbrodt, J., Thumberger, T. (2019) Swift Large-scale Examination of Directed Genome Editing. PLoS One. 14:e0213317

Knickmeyer, M.D., Mateo, J.L., Eckert, P., Roussa, E., Rahhal, B., Zuniga, A., Krieglstein, K., Wittbrodt, J., Heermann, S. (2018) TGFβ-facilitated optic fissure fusion and the role of bone morphogenetic protein antagonism. Open Biology. 8(3)

Lust, K., Wittbrodt, J. (2018) Activating the regenerative potential of Müller glia cells in a regeneration-deficient retina. eLIFE. 7

Kuri, P., Schieber, N.L., Thumberger, T., Wittbrodt, J., Schwab, Y., Leptin, M. (2017) Dynamics of in vivo ASC speck formation. The Journal of cell biology. 216(9):2891-2909

Nicolás-Pérez, M., Kuchling, F., Letelier, J., Polvillo, R., Wittbrodt, J.,Martínez-Morales, J.R. (2016) Analysis of cellular behavior and cytoskeletal dynamics reveal a constriction mechanism driving optic cup morphogenesis. eLIFE. 5

Kromm, D., Thumberger, T., Wittbrodt, J. (2016) An eye on light-sheet microscopy. Methods in cell biology. 133:105-123

Auer, T.O., Xiao, T., Bercier, V., Gebhardt, C., Duroure, K., Concordet, J.P., Wyart, C., Suster, M., Kawakami, K., Wittbrodt, J., Baier, H., Del Bene, F. (2015) Deletion of a kinesin I motor unmasks a mechanism of homeostatic branching control by neurotrophin-3. eLIFE. 4

Heermann, S., Schütz, L., Lemke, S., Krieglstein, K., Wittbrodt, J. (2015) Eye morphogenesis driven by epithelial flow into the optic cup facilitated by modulation of bone morphogenetic protein. eLIFE. 4

Mazaheri, F., Breus, O., Durdu, S., Haas, P., Wittbrodt, J., Gilmour, D., Peri, F. (2014) Distinct roles for BAI1 and TIM-4 in the engulfment of dying neurons by microglia. Nature communications. 5:4046

Schulz, S., Chachami, G., Kozaczkiewicz, L., Winter, U., Stankovic-Valentin, N., Haas, P., Hofmann, K., Urlaub, H., Ovaa, H., Wittbrodt, J., Meulmeester, E., and Melchior, F. (2012) Ubiquitin-specific protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions. EMBO reports. 13(10):930-938

Harden, M.V., Pereiro, L., Ramialison, M., Wittbrodt, J., Prasad, M.K., McCallion, A.S., and Whitlock, K.E. (2012) Close association of olfactory placode precursors and cranial neural crest cells does not predestine cell mixing. Developmental Dynamics : an official publication of the American Association of Anatomists. 241(7):1143-1154

Centanin, L., Hoeckendorf, B., Wittbrodt, J. (2011) Fate restriction and multipotency in retinal stem cells. Cell Stem Cell. 9:553-62

Keller, P.J., Schmidt, A.D., Wittbrodt, J., and Stelzer, E.H. (2011) Digital Scanned Laser Light-Sheet Fluorescence Microscopy (DSLM) of Zebrafish and Drosophila Embryonic Development. Cold Spring Harbor protocols. 2011(10):1235-43

Maurya, A.K., Tan, H., Souren, M., Wang, X., Wittbrodt, J., and Ingham, P.W. (2011) Integration of Hedgehog and BMP signalling by the engrailed2a gene in the zebrafish myotome. Development (Cambridge, England). 138(4):755-765

Signore, I.A., Guerrero, N., Loosli, F., Colombo, A., Villalón, A., Wittbrodt, J., and Concha, M.L. (2009) Zebrafish and medaka: model organisms for a comparative developmental approach of brain asymmetry. Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 364(1519):991-1003

Keller, P.J., Schmidt, A.D., Wittbrodt, J., and Stelzer, E.H. (2008) Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet Microscopy. Science (New York, N.Y.). 322(5904):1065-1069

Grabher, C., and Wittbrodt, J. (2008) Recent advances in meganuclease-and transposon-mediated transgenesis of medaka and zebrafish. Methods in molecular biology (Clifton, N.J.). 461:521-539

Yokoi, H., Shimada, A., Carl, M., Takashima, S., Kobayashi, D., Narita, T., Jindo, T., Kimura, T., Kitagawa, T., Kage, T., Sawada, A., Naruse, K., Asakawa, S., Shimizu, N., Mitani, H., Shima, A., Tsutsumi, M., Hori, H., Wittbrodt, J., Saga, Y., Ishikawa, Y., Araki, K., and Takeda, H. (2007) Mutant analyses reveal different functions of fgfr1 in medaka and zebrafish despite conserved ligand-receptor relationships. Developmental Biology. 304(1):326-337

Grabher, C., and Wittbrodt, J. (2007) Meganuclease and transposon mediated transgenesis in medaka. Genome biology. 8(1):S10

Ason, B., Darnell, D.K., Wittbrodt, B., Berezikov, E., Kloosterman, W.P., Wittbrodt, J., Antin, P.B., and Plasterk, R.H. (2006) Differences in vertebrate microRNA expression. Proceedings of the National Academy of Sciences of the United States of America. 103(39):14385-14389

Rembold, M., Loosli, F., Adams, R. J., Wittbrodt, J. (2006) Individual Cell Migration Serves as the Driving Force for Optic Vesicle Evagination. Science (New York, N.Y.). 313:1130-1134

Rembold, M., Lahiri, K., Foulkes, N.S., and Wittbrodt, J. (2006) Transgenesis in fish: efficient selection of transgenic fish by co-injection with a fluorescent reporter construct. Nature Protocols. 1(3):1133-1139

Schäfer, M., Rembold, M., Wittbrodt, J., Schartl, M., and Winkler, C. (2005) Medial floor plate formation in zebrafish consists of two phases and requires trunk-derived Midkine-a. Genes & Development. 19(8):897-902

Furutani-Seiki, M., and Wittbrodt, J. (2004) Medaka and zebrafish, an evolutionary twin study. Mechanisms of Development. 121(7-8):629-637

Henrich, T., Ramialison, M., Segerdell, E., Westerfield, M., Furutani-Seiki, M., Wittbrodt, J., Kondoh, H. (2004) GSD: a genetic screen database. Mechanisms of Development. 121(7-8):959-963

Grabher, C., Joly, J.S., and Wittbrodt, J. (2004) Highly efficient zebrafish transgenesis mediated by the meganuclease I-SceI. The Zebrafish: Genetics, Genomics and Informatics, 2nd ed., Methods Cell Biol.. 77:381-401

Loosli, F., Staub, W., Finger-Baier, K.C., Ober, E.A., Verkade, H., Wittbrodt, J., and Baier, H. (2003) Loss of eyes in zebrafish caused by mutation of chokh/rx3. EMBO reports. 4(9):894-899

Hammerschmidt, M., Kramer, C., Nowak, M., Herzog, W., and Wittbrodt, J. (2003) Loss of maternal Smad5 in zebrafish embryos affects patterning and morphogenesis of optic primordia. Developmental Dynamics : an official publication of the American Association of Anatomists. 227(1):128-133

Thermes, V., Grabher, C., Ristoratore, F., Bourrat, F., Choulika, A., Wittbrodt, J., and Joly, J.S. (2002) I-SceI meganuclease mediates highly efficient transgenesis in fish. Mechanisms of Development. 118(1-2):91-98

Adamska, M., Wolff, A., Kreusler, M., Wittbrodt, J., Braun, T., and Bober, E. (2001) Five Nkx5 genes show differential expression patterns in anlagen of sensory organs in medaka: insight into the evolution of the gene family. Development genes and evolution. 211(7):338-349

Delot, E., Kataoka, H., Goutel, C., Yan, Y.L., Postlethwait, J., Wittbrodt, J., and Rosa, F.M. (1999) The BMP-related protein Radar: a maintenance factor for dorsal neuroectoderm cells?. Mechanisms of Development. 85(1-2):15-25

Rissi, M., Wittbrodt, J., Delot, E., Naegeli, M., and Rosa, F.M. (1995) Zebrafish Radar: a new member of the TGF-ß superfamily defines dorsal regions of the neural plate and the embryonic retina. Mechanisms of Development. 49(3):223-34

Wittbrodt, J. and Rosa, F.M. (1994) Disruption of mesoderm and axis formation in fish by ectopic expression of activin variants: the role of maternal activin. Genes & Development. 8:1448-1462

NON-ZEBRAFISH PUBLICATIONS
Watanabe, T., Asaka, S., Kitagawa, D., Saito, K., Kurashige, R., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Henrich, T., Hirose, Y., Yasuoka, A., Yoda, H., Deguchi, T., Iwanami, N., Kunimatsu, S., Osakada, M., Loosli, F., Quiring, R., Carl, M., Grabher, C., Winkler, S., Del Bene, F., Wittbrodt, J., Abe, K., Takahama, Y., Takahashi, K., Katada, T., Nishina, H., Kondoh, H., and Furutani-Seiki, M. (2004) Mutations affecting liver development and function in Medaka, Oryzias latipes, screened by multiple criteria. Mech. Dev.. 121(7-8):791-802

Loosli, F., Bene, F.D., Quiring, R., Rembold, M., Martinez-Morales, J.R., Carl, M., Grabher, C., Iquel, C., Krone, A., Wittbrodt, B., Winkler, S., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Henrich, T., Deguchi, T., Hirose, Y., Iwanami, N., Kunimatsu, S., Osakada, M., Watanabe, T., Yasuoka, A., Yoda, H., Winkler, C., Elmasri, H., Kondoh, H., Furutani-Seiki, M., and Wittbrodt, J. (2004) Mutations affecting retina development in Medaka. Mech. Dev.. 121(7-8):703-714

Kitagawa, D., Watanabe, T., Saito, K., Asaka, S., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Yasuoka, A., Deguchi, T., Yoda, H., Hirose, Y., Henrich, T., Iwanami, N., Kunimatsu, S., Osakada, M., Winkler, C., Elmasri, H., Wittbrodt, J., Loosli, F., Quiring, R., Carl, M., Grabher, C., Winkler, S., Del Bene, F., Momoi, A., Katada, T., Nishina, H., Kondoh, H., and Furutani-Seiki, M. (2004) Genetic dissection of the formation of the forebrain in Medaka, Oryzias latipes. Mech. Dev.. 121(7-8):673-685

Elmasri, H., Winkler, C., Liedtke, D., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Henrich, T., Hirose, Y., Yasuoka, A., Yoda, H., Watanabe, T., Deguchi, T., Iwanami, N., Kunimatsu, S., Osakada, M., Loosli, F., Quiring, R., Carl, M., Grabher, C., Winkler, S., Del Bene, F., Wittbrodt, J., Abe, K., Takahama, Y., Takahashi, K., Katada, T., Nishina, H., Kondoh, H., and Furutani-Seiki, M. (2004) Mutations affecting somite formation in the Medaka (Oryzias latipes). Mech. Dev.. 121(7-8):659-671

Furutani-Seiki, M., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Yoda, H., Deguchi, T., Hirose, Y., Yasuoka, A., Henrich, T., Watanabe, T., Iwanami, N., Kitagawa, D., Saito, K., Asaka, S., Osakada, M., Kunimatsu, S., Momoi, A., Elmasri, H., Winkler, C., Ramialison, M., Loosli, F., Quiring, R., Carl, M., Grabher, C., Winkler, S., Del Bene, F., Shinomiya, A., Kota, Y., Yamanaka, T., Okamoto, Y., Takahashi, K., Todo, T., Abe, K., Takahama, Y., Tanaka, M., Mitani, H., Katada, T., Nishina, H., Nakajima, N., Wittbrodt, J., and Kondoh, H. (2004) A systematic genome-wide screen for mutations affecting organogenesis in Medaka, Oryzias latipes. Mech. Dev.. 121(7-8):647-658

Wittbrodt, J., Shima, A., and Schartl, M. (2002) Medaka--a model organism from the far East. Nat. Rev. Genet.. 3(1):53-64

Loosli, F., Winkler, S., Burgtorf, C., Wurmbach, E., Ansorge, W., Henrich, T., Grabher, C., Arendt, D., Carl, M., Krone, A., Grzebisz, E., and Wittbrodt, J. (2001) Medaka eyeless is the key factor linking retinal determination and eye growth. Development. 128(20):4035-4044

Loosli, F., Köster, R.W., Carl, M., Kuhnlein, R., Henrich, T., Mucke, M., Krone, A., and Wittbrodt, J. (2000) A genetic screen for mutations affecting embryonic development in medaka fish (Oryzias latipes). Mech. Dev.. 97(1-2):133-139

Köster, R.W., Kuhnlein, R.P., and Wittbrodt, J. (2000) Ectopic Sox3 activity elicits sensory placode formation. Mech. Dev. 95(1-2):175-187

Carl, M., and Wittbrodt, J. (1999) Graded interference with FGF signalling reveals its dorsoventral asymmetry at the mid-hindbrain boundary. Development. 126(24):5659-5667

Ristoratore, F., Carl, M., Deschet, K., Richard-Parpaillon, L., Boujard, D., Wittbrodt, J., Chourrout, D., Bourrat, F., and Joly, J.S. (1999) The midbrain-hindbrain boundary genetic cascade is activated ectopically in the diencephalon in response to the widespread expression of one of its components, the medaka gene Ol-eng2. Development. 126(17):3769-3779

Loosli, F., Köster, R.W., Carl, M., Krone, A., and Wittbrodt, J. (1998) Six3, a medaka homologue of the Drosophila homeobox gene sine oculis is expressed in the anterior embryonic shield and the developing eye. Mech. Dev.. 74(1-2):159-164

Köster, R.W., Stick, R., Loosli, F., and Wittbrodt, J. (1997) Medaka spalt acts as a target gene of hedgehog signaling. Development. 124(16):3147-3156

Oliver, G., Loosli, F., Köster, R.W., Wittbrodt, J., and Gruss, P. (1996) Ectopic lens induction in fish in response to the murine homeobox gene Six3. Mech. Dev.. 60(2):233-239