PUBLICATION
Chemokine-guided angiogenesis directs coronary vasculature formation in zebrafish
- Authors
- Harrison, M.R., Bussmann, J., Huang, Y., Zhao, L., Osorio, A., Burns, C.G., Burns, C.E., Sucov, H.M., Siekmann, A.F., Lien, C.L.
- ID
- ZDB-PUB-150529-6
- Date
- 2015
- Source
- Developmental Cell 33: 442-54 (Journal)
- Registered Authors
- Burns (Erter), Caroline, Burns, Geoff, Bussmann, Jeroen, Harrison, Michael, Lien, Ching-Ling (Ellen), Siekmann, Arndt Friedrich, Sucov, Henry M., Zhao, Long
- Keywords
- none
- MeSH Terms
-
- Organogenesis/physiology
- Heart/physiology
- Regeneration/physiology
- Endothelium, Vascular/cytology
- Endothelium, Vascular/metabolism
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism
- Angiography
- Animals
- Microscopy, Confocal
- Coronary Vessels/embryology
- Coronary Vessels/growth & development*
- Gene Expression Regulation, Developmental
- Animals, Genetically Modified/genetics
- Animals, Genetically Modified/growth & development*
- Animals, Genetically Modified/metabolism
- Receptors, CXCR4/metabolism*
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism*
- Zebrafish Proteins/metabolism*
- Neovascularization, Physiologic*
- Signal Transduction
- Chemokines, CXC/metabolism*
- PubMed
- 26017769 Full text @ Dev. Cell
Citation
Harrison, M.R., Bussmann, J., Huang, Y., Zhao, L., Osorio, A., Burns, C.G., Burns, C.E., Sucov, H.M., Siekmann, A.F., Lien, C.L. (2015) Chemokine-guided angiogenesis directs coronary vasculature formation in zebrafish. Developmental Cell. 33:442-54.
Abstract
Interruption of the coronary blood supply severely impairs heart function with often fatal consequences for patients. However, the formation and maturation of these coronary vessels is not fully understood. Here we provide a detailed analysis of coronary vessel development in zebrafish. We observe that coronary vessels form in zebrafish by angiogenic sprouting of arterial cells derived from the endocardium at the atrioventricular canal. Endothelial cells express the CXC-motif chemokine receptor Cxcr4a and migrate to vascularize the ventricle under the guidance of the myocardium-expressed ligand Cxcl12b. cxcr4a mutant zebrafish fail to form a vascular network, whereas ectopic expression of Cxcl12b ligand induces coronary vessel formation. Importantly, cxcr4a mutant zebrafish fail to undergo heart regeneration following injury. Our results suggest that chemokine signaling has an essential role in coronary vessel formation by directing migration of endocardium-derived endothelial cells. Poorly developed vasculature in cxcr4a mutants likely underlies decreased regenerative potential in adults.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping