ZFIN ID: ZDB-PUB-150113-7
Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells
Qiao, J., Cui, S.J., Xu, L.L., Chen, S.J., Yao, J., Jiang, Y.H., Peng, G., Fang, C.Y., Yang, P.Y., Liu, F.
Date: 2015
Source: Oncotarget   6(2): 1171-89 (Journal)
Registered Authors: Chen, Sijie, Liu, Feng, Peng, Gang
Keywords: none
MeSH Terms:
  • Adult
  • Aged
  • Aged, 80 and over
  • Cell Line, Tumor
  • Cell Movement/genetics
  • Cell Proliferation/genetics
  • Chromatography, Liquid
  • Female
  • Filamins/genetics
  • Filamins/metabolism*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Immunohistochemistry
  • Male
  • Mass Spectrometry
  • Middle Aged
  • Neoplasms/genetics
  • Neoplasms/metabolism*
  • Neoplasms/pathology
  • Proteome/genetics
  • Proteome/metabolism*
  • Proteomics/methods*
  • RNA Interference
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stomach Neoplasms/genetics
  • Stomach Neoplasms/metabolism*
  • Stomach Neoplasms/pathology
PubMed: 25577646 Full text @ Oncotarget
Gastric cancer (GC) is the fourth and fifth most common cancer in men and women, respectively. We identified 2,750 proteins at false discovery rates of 1.3% (protein) and 0.03% (spectrum) by comparing the proteomic profiles of three GC and a normal gastric cell lines. Nine proteins were significantly dysregulated in all three GC cell lines, including filamin C, a muscle-specific filamin and a large actin-cross-linking protein. Downregulation of filamin C in GC cell lines and tissues were verified using quantitative PCR and immunohistochemistry. Data-mining using public microarray datasets shown that filamin C was significantly reduced in many human primary and metastasis cancers. Transient expression or silencing of filamin C affected the proliferation and colony formation of cancer cells. Silencing of endogenous filamin C enhanced cancer cell migration and invasion, whereas ectopic expression of filamin C had opposing effects. Silencing of filamin C increased the expression of matrix metallopeptidase 2 and improved the metastasis of prostate cancer in a zebrafish model. High filamin C associated with better prognosis of prostate cancer, leukemia and breast cancer patients. These findings establish a functional role of filamin C in human cancers and these data will be valuable for further study of its mechanisms.