PUBLICATION

Out with the old, in with the new: reassessing morpholino knockdowns in light of genome editing technology

Authors
Schulte-Merker, S., Stainier, D.Y.
ID
ZDB-PUB-140808-5
Date
2014
Source
Development (Cambridge, England)   141: 3103-4 (Other)
Registered Authors
Schulte-Merker, Stefan, Stainier, Didier
Keywords
none
MeSH Terms
  • Animals
  • Genetic Techniques*/trends
  • Genome
  • Morpholinos/genetics*
  • Mutation
  • Phenotype
  • Zebrafish
PubMed
25100652 Full text @ Development
Abstract
Morpholino oligomers have been used widely and for many years in the zebrafish community to transiently knock down the function of target genes. It has often been difficult, however, to reliably discriminate between specific and non-specific effects, and thus generally accepted guidelines to control for morpholino side effects do not exist. In light of recent methodologies to generate mutant lines in virtually any zebrafish gene, we discuss these different approaches with a specific focus on how the first description of a loss-of-function phenotype in zebrafish should be accomplished.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes