ZFIN ID: ZDB-PUB-140808-5
Out with the old, in with the new: reassessing morpholino knockdowns in light of genome editing technology
Schulte-Merker, S., Stainier, D.Y.
Date: 2014
Source: Development (Cambridge, England) 141: 3103-4 (Other)
Registered Authors: Schulte-Merker, Stefan, Stainier, Didier
Keywords: none
MeSH Terms: Animals; Genetic Techniques*/trends; Genome; Morpholinos/genetics*; Mutation (all 7) expand
PubMed: 25100652 Full text @ Development
ABSTRACT
Morpholino oligomers have been used widely and for many years in the zebrafish community to transiently knock down the function of target genes. It has often been difficult, however, to reliably discriminate between specific and non-specific effects, and thus generally accepted guidelines to control for morpholino side effects do not exist. In light of recent methodologies to generate mutant lines in virtually any zebrafish gene, we discuss these different approaches with a specific focus on how the first description of a loss-of-function phenotype in zebrafish should be accomplished.
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