PUBLICATION

Histopathologic alterations associated with global gene expression due to chronic dietary TCDD exposure in juvenile zebrafish

Authors
Liu, Q., Spitsbergen, J.M., Cariou, R., Huang, C.Y., Jiang, N., Goetz, G., Hutz, R.J., Tonellato, P.J., Carvan, M.J.
ID
ZDB-PUB-140706-21
Date
2014
Source
PLoS One   9: e100910 (Journal)
Registered Authors
Carvan III, Michael J., Spitsbergen, Jan
Keywords
none
Datasets
GEO:GSE47434, GEO:GSE47547, GEO:GSE47549
MeSH Terms
  • Animals
  • Biomarkers/metabolism
  • Environmental Pollutants/adverse effects*
  • Environmental Pollutants/pharmacology
  • Female
  • Gene Expression Regulation, Developmental/drug effects*
  • Genomics
  • Male
  • Oligonucleotide Array Sequence Analysis
  • Organ Specificity/drug effects
  • Zebrafish/embryology*
PubMed
24988445 Full text @ PLoS One
Abstract
The goal of this project was to investigate the effects and possible developmental disease implication of chronic dietary TCDD exposure on global gene expression anchored to histopathologic analysis in juvenile zebrafish by functional genomic, histopathologic and analytic chemistry methods. Specifically, juvenile zebrafish were fed Biodiet starter with TCDD added at 0, 0.1, 1, 10 and 100 ppb, and fish were sampled following 0, 7, 14, 28 and 42 d after initiation of the exposure. TCDD accumulated in a dose- and time-dependent manner and 100 ppb TCDD caused TCDD accumulation in female (15.49 ppb) and male (18.04 ppb) fish at 28 d post exposure. Dietary TCDD caused multiple lesions in liver, kidney, intestine and ovary of zebrafish and functional dysregulation such as depletion of glycogen in liver, retrobulbar edema, degeneration of nasal neurosensory epithelium, underdevelopment of intestine, and diminution in the fraction of ovarian follicles containing vitellogenic oocytes. Importantly, lesions in nasal epithelium and evidence of endocrine disruption based on alternatively spliced vasa transcripts are two novel and significant results of this study. Microarray gene expression analysis comparing vehicle control to dietary TCDD revealed dysregulated genes involved in pathways associated with cardiac necrosis/cell death, cardiac fibrosis, renal necrosis/cell death and liver necrosis/cell death. These baseline toxicological effects provide evidence for the potential mechanisms of developmental dysfunctions induced by TCDD and vasa as a biomarker for ovarian developmental disruption.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes