PUBLICATION
Chemically Induced Intestinal Damage Models in Zebrafish Larvae
- Authors
- Oehlers, S.H., Flores, M.V., Hall, C.J., Okuda, K.S., Sison, J.O., Crosier, K.E., and Crosier, P.S.
- ID
- ZDB-PUB-130312-33
- Date
- 2013
- Source
- Zebrafish 10(2): 184-93 (Journal)
- Registered Authors
- Crosier, Kathy, Crosier, Phil, Flores, Maria, Hall, Chris, Oehlers, Stefan, Okuda, Kazuhide Shaun
- Keywords
- none
- MeSH Terms
-
- Enterocolitis/chemically induced*
- Enterocolitis/drug therapy
- Enterocolitis/immunology
- Enterocolitis/microbiology
- Neutrophils/immunology
- Anti-Inflammatory Agents/administration & dosage
- Neutral Red/metabolism
- Dextran Sulfate/pharmacology*
- Animals
- Flow Cytometry
- Inflammation/chemically induced*
- Inflammation/drug therapy
- Inflammation/immunology
- Inflammation/microbiology
- Inflammatory Bowel Diseases/chemically induced*
- Inflammatory Bowel Diseases/drug therapy
- Inflammatory Bowel Diseases/immunology
- Inflammatory Bowel Diseases/microbiology
- Larva
- Zebrafish Proteins/genetics
- Zebrafish Proteins/immunology
- Zebrafish Proteins/metabolism
- Anti-Bacterial Agents/administration & dosage
- Polymerase Chain Reaction
- Immersion
- Nitric Oxide/metabolism
- Intestines/microbiology
- Intestines/pathology
- Interleukin-23/genetics
- Interleukin-23/immunology
- Interleukin-23/metabolism
- Zebrafish*
- Trinitrobenzenesulfonic Acid/pharmacology*
- Drug Discovery
- Disease Models, Animal*
- Humans
- Microinjections
- Alcian Blue/metabolism
- Morpholinos/administration & dosage
- Optical Imaging
- PubMed
- 23448252 Full text @ Zebrafish
Citation
Oehlers, S.H., Flores, M.V., Hall, C.J., Okuda, K.S., Sison, J.O., Crosier, K.E., and Crosier, P.S. (2013) Chemically Induced Intestinal Damage Models in Zebrafish Larvae. Zebrafish. 10(2):184-93.
Abstract
Several intestinal damage models have been developed using zebrafish, with the aim of recapitulating aspects of human inflammatory bowel disease (IBD). These experimentally induced inflammation models have utilized immersion exposure to an array of colitogenic agents (including live bacteria, bacterial products, and chemicals) to induce varying severity of inflammation. This technical report describes methods used to generate two chemically induced intestinal damage models using either dextran sodium sulfate (DSS) or trinitrobenzene sulfonic acid (TNBS). Methods to monitor intestinal damage and inflammatory processes, and chemical-genetic methods to manipulate the host response to injury are also described.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping