PUBLICATION
Chemically Induced Intestinal Damage Models in Zebrafish Larvae
- Authors
- Oehlers, S.H., Flores, M.V., Hall, C.J., Okuda, K.S., Sison, J.O., Crosier, K.E., and Crosier, P.S.
- ID
- ZDB-PUB-130312-33
- Date
- 2013
- Source
- Zebrafish 10(2): 184-93 (Journal)
- Registered Authors
- Crosier, Kathy, Crosier, Phil, Flores, Maria, Hall, Chris, Oehlers, Stefan, Okuda, Kazuhide Shaun
- Keywords
- none
- MeSH Terms
-
- Humans
- Animals
- Anti-Bacterial Agents/administration & dosage
- Nitric Oxide/metabolism
- Microinjections
- Morpholinos/administration & dosage
- Alcian Blue/metabolism
- Anti-Inflammatory Agents/administration & dosage
- Neutrophils/immunology
- Flow Cytometry
- Optical Imaging
- Polymerase Chain Reaction
- Dextran Sulfate/pharmacology*
- Interleukin-23/genetics
- Interleukin-23/immunology
- Interleukin-23/metabolism
- Zebrafish*
- Immersion
- Neutral Red/metabolism
- Enterocolitis/chemically induced*
- Enterocolitis/drug therapy
- Enterocolitis/immunology
- Enterocolitis/microbiology
- Trinitrobenzenesulfonic Acid/pharmacology*
- Inflammation/chemically induced*
- Inflammation/drug therapy
- Inflammation/immunology
- Inflammation/microbiology
- Larva
- Drug Discovery
- Inflammatory Bowel Diseases/chemically induced*
- Inflammatory Bowel Diseases/drug therapy
- Inflammatory Bowel Diseases/immunology
- Inflammatory Bowel Diseases/microbiology
- Zebrafish Proteins/genetics
- Zebrafish Proteins/immunology
- Zebrafish Proteins/metabolism
- Disease Models, Animal*
- Intestines/microbiology
- Intestines/pathology
- PubMed
- 23448252 Full text @ Zebrafish
Citation
Oehlers, S.H., Flores, M.V., Hall, C.J., Okuda, K.S., Sison, J.O., Crosier, K.E., and Crosier, P.S. (2013) Chemically Induced Intestinal Damage Models in Zebrafish Larvae. Zebrafish. 10(2):184-93.
Abstract
Several intestinal damage models have been developed using zebrafish, with the aim of recapitulating aspects of human inflammatory bowel disease (IBD). These experimentally induced inflammation models have utilized immersion exposure to an array of colitogenic agents (including live bacteria, bacterial products, and chemicals) to induce varying severity of inflammation. This technical report describes methods used to generate two chemically induced intestinal damage models using either dextran sodium sulfate (DSS) or trinitrobenzene sulfonic acid (TNBS). Methods to monitor intestinal damage and inflammatory processes, and chemical-genetic methods to manipulate the host response to injury are also described.
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