PUBLICATION

In vivo Wnt signaling tracing through a transgenic biosensor fish reveals novel activity domains

Authors
Moro, E., Özhan, G., Mongera, A., Beis, D., Wierzbicki, C., Young, R.M., Bournele, D., Domenichini, A., Valdivia, L.E., Lum, L., Chen, C., Amatruda, J.F., Tiso, N., Weidinger, G., and Argenton, F.
ID
ZDB-PUB-120503-17
Date
2012
Source
Developmental Biology   366(2): 327-340 (Journal)
Registered Authors
Amatruda, James F., Argenton, Francesco, Beis, Dimitris, Domenichini, Alice, Mongera, Alessandro, Moro, Enrico, Özhan, Günes, Tiso, Natascia, Weidinger, Gilbert, Young, Rodrigo
Keywords
zebrafish, wnt/β-catenin, reporter, confocal microscopy, small-chemicals, biosensor
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Biosensing Techniques
  • Cell Differentiation
  • Cell Movement
  • Neurons/cytology
  • Neurons/physiology
  • Wnt Signaling Pathway*
  • Zebrafish/embryology
  • Zebrafish/physiology*
PubMed
22546689 Full text @ Dev. Biol.
Abstract

The creation of molecular tools able to unravel in vivo spatiotemporal activation of specific cell signaling events during cell migration, differentiation and morphogenesis is of great relevance to developmental cell biology. Here, we describe the generation, validation and applications of two transgenic reporter lines for Wnt/β-catenin signaling, named TCFsiam, and show that they are reliable and sensitive Wnt biosensors for in vivo studies. We demonstrate that these lines sensitively detect Wnt/β-catenin pathway activity in several cellular contexts, from sensory organs to cardiac valve patterning. We provide evidence that Wnt/β-catenin activity is involved in the formation and maintenance of the zebrafish CNS blood vessel network, on which sox10 neural crest-derived cells migrate and proliferate. We finally show that these transgenic lines allow for screening of Wnt signaling modifying compounds, tissue regeneration assessment as well as evaluation of potential Wnt/β-catenin genetic modulators.

Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping