PUBLICATION

Analysis of Pax7 expressing myogenic cells in zebrafish muscle development, injury, and models of disease

Authors
Seger, C., Hargrave, M., Wang, X., Chai, R.J., Elworthy, S., and Ingham, P.W.
ID
ZDB-PUB-111013-7
Date
2011
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   240(11): 2440-51 (Journal)
Registered Authors
Elworthy, Stone, Hargrave, Murray, Ingham, Philip, Seger, Claudia, Wang, Xingang
Keywords
Pax7, Pax3, Myod, GFP-reporter, dystrophin, integrin alpha7, muscle progenitor cell, satellite cell, muscle injury, repair, zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Humans
  • PAX7 Transcription Factor/genetics*
  • PAX7 Transcription Factor/metabolism
  • Embryo, Nonmammalian
  • Muscular Diseases/chemically induced
  • Muscular Diseases/genetics*
  • Muscular Diseases/metabolism
  • Muscular Diseases/pathology
  • Disease Models, Animal*
  • Muscular Dystrophy, Duchenne/genetics
  • Muscular Dystrophy, Duchenne/metabolism
  • Muscular Dystrophy, Duchenne/pathology
  • Muscle, Skeletal/injuries
  • Muscle, Skeletal/metabolism
  • Myoblasts/metabolism*
  • Myoblasts/physiology
  • Genes, Reporter/physiology
  • Gene Expression Regulation, Developmental
  • Cobra Cardiotoxin Proteins
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Muscle Development/genetics*
(all 25)
PubMed
21954137 Full text @ Dev. Dyn.
Abstract
The transcription factor Pax7 is a marker and regulator of muscle progenitors and satellite cells that contribute to the embryonic development and postembryonic growth of skeletal muscle in vertebrates, as well as to its repair and regeneration. Here, we identify Pax7+ve myogenic cells in the zebrafish and characterize their behavior in postembryonic stages. Mononucleate Pax7+ve cells can first be found associated with myofibers at 72 hours post fertilization (hpf). To follow the behavior of muscle progenitor cells in vivo, we generated transgenic lines expressing fluorescent proteins under the control of the pax7a or pax3a promoters. We established an injury model using cardiotoxin injection and monitored cell proliferation and myogenic regulatory factor expression in myogenic precursors cells and muscle fibers after injury using proliferation markers and the transgenic lines. We also analyzed Pax7+ve cells in animals with dystrophic phenotypes and found an increased number compared with wild-type.
Genes / Markers
Figures
Figure Gallery (10 images)
Show all Figures
Expression
Phenotype
No data available
Mutations / Transgenics
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
itga7MO3-itga7MRPHLNO
myod1MO1-myod1MRPHLNO
1 - 2 of 2
Show
Fish
Fish
dmdta222a/ta222a
i124Tg
i131Tg
i150Tg
i167Tg
smb237Et
y1Tg
1 - 7 of 7
Show
Antibodies
Orthology
No data available
Engineered Foreign Genes
Marker Marker Type Name
EGFPEFGEGFP
TomatoEFGTomato
YFPEFGYFP
1 - 3 of 3
Show
Mapping
No data available
Your Input Welcome
We welcome your input and comments. Please use this form to recommend updates to the information in ZFIN. We appreciate as much detail as possible and references as appropriate. We will review your comments promptly.
Citation
Seger, C., Hargrave, M., Wang, X., Chai, R.J., Elworthy, S., and Ingham, P.W. (2011) Analysis of Pax7 expressing myogenic cells in zebrafish muscle development, injury, and models of disease. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(11):2440-51.