|ZFIN ID: ZDB-PERS-050429-1|
BIOGRAPHY AND RESEARCH INTERESTS
I completed by Bachelor of Science in 1991 at the University of Queensland in Brisbane, Australia. In 1992 I gained an Honours Degree in Biochemistry at the same university by undertaking a short project analysing the expression of the cystic fibrosis transmembrane conductance regulator (CFTR) gene in mouse development. This project was carried out in Prof. Brandon Wainwright’s laboratory at the Centre for Molecular and Cellular Biology (CMCB). I then joined Prof. Peter Koopman’s laboratory at the CMCB and focussed on the cloning of mouse Sox transcription factor genes and characterisation of their expression during organogenesis. I was awarded my PhD in 1999. Because of the expression of many Sox genes in the central nervous system (CNS), I became interested in the molecular genetics of CNS development and took up a post-Doctoral position in the laboratory of Dr. Toshiya Yamada at the Institute for Molecular Bioscience (IMB), University of Queensland. We were investigating the patterning and differentiation of embryonic CNS neurons in mouse and chicken embryos by examining the expression of subclass-specific transcription factors and through manipulation hedgehog signalling. In 2001, Dr Yamada died and I continued in his laboratory as acting principal investigator. In 2003, I joined Prof. Philip Ingham’s as a Post-Doc and I’m currently working on muscle patterning and differentiation and the hedgehog signalling pathway in zebrafish.
Seger, C., Hargrave, M., Wang, X., Chai, R.J., Elworthy, S., and Ingham, P.W. (2011) Analysis of Pax7 expressing myogenic cells in zebrafish muscle development, injury, and models of disease. Developmental dynamics : an official publication of the American Association of Anatomists. 240(11):2440-51
Cerda, G.A., Hargrave, M., and Lewis, K.E. (2009) RNA profiling of FAC-sorted neurons from the developing zebrafish spinal cord. Developmental dynamics : an official publication of the American Association of Anatomists. 238(1):150-161
Elworthy, S., Hargrave, M., Knight, R., Mebus, K., and Ingham, P.W. (2008) Expression of multiple slow myosin heavy chain genes reveals a diversity of zebrafish slow twitch muscle fibres with differing requirements for Hedgehog and Prdm1 activity. Development (Cambridge, England). 135(12):2115-2126
Karunaratne, A., Hargrave, M., Poh A. and Yamada T. (2002) GATA proteins identify a novel ventral interneuron subclass in the developing chick spinal cord. Dev. Biol. 249:30-43
Poh, A., Karunaratne, A., Kolle, G., Huang, N., Smith, E., Starkey, J., Wen, D., Wilson, I., Yamada, T. and Hargrave, M. (2002) Patterning of the vertebrate ventral spinal cord. Int J. Dev. Biol. 46:597-608
Smith, E., Hargrave, M., Yamada, T., Begley, C., G. and Little, M (2002) Co-expression of SCL and GATA3 in the V2 interneurons of the developing mouse spinal cord. Dev. Dyn. 224:231-237
Hargrave, M., James, K., Nield K., Toomes, C., Georgas, K., Sullivan T., Verzijl, H.T.F.M., Oley, C.A., Little M., De Jonghe, P., Kwon, J.M., Kremer, H., Dixon, M.J. Timmerman, V., Yamada, T., and Koopman P. (2000) Fine mapping of the neurally-expressed gene SOX14 to human 3q23, relative to three congenital diseases. Hum. Genet. 106:432-439
Hargrave, M., James, K., Yamada T., and Koopman, P. (2000) Sox14 maps to mouse chromosome 9 and shows no mutations in the neurological mouse mutants ducky and tippy. Mamm. Genome 11:231-233
Hargrave, M., Karunaratne, A., Cox, L., Wood, S., Koopman, P. and Yamada, T. (2000) The HMG box transcription factor gene Sox14 marks a novel subset of ventral interneurons and is regulated by sonic hedgehog. Dev. Biol. 219:142-153
Hargrave, M. and Koopman, P. (2000) In situ hybridisation of whole mount embryos. In: In Situ Hybridization Protocols, 2nd ed. Methods in Molecular Biology, Vol 123; IA Darby, ed. Humana Press, Totowa, NJ. pp. 279-89
Yamada, T., Karunaratne, A. and Hargrave, M. (1999) Molecular control of cell type diversity in the developing spinal cord. Clin. Exp. Pharmacol. P. 26:741-745
Hargrave, M.R., Wright, E., Kun, J., Emery, J., Cooper, L. and Koopman, P. (1997) Expression of the Sox11 gene in mouse embryos suggests roles in neuronal maturation and epithelio-mesenchymal induction. Dev. Dyn. 210:79-86
Wright, E., Hargrave, M.R., Christiansen, J., Cooper, L., Kun, J., Evans, T., Gangadharan, U., Greenfield, A. and Koopman, P. (1995) The Sry-related gene Sox-9 is expressed during chondrogenesis in mouse embryos. Nat. Genet. 9:15-20.
Delaney, S.J., Rich, D.P., Thomson, S.A., Hargrave, M.R., Lovelock, P.K., Welsh, M.J. and Wainwright, B.J. (1993) Cystic fibrosis transmembrane conductance regulator splice variants are not conserved and fail to produce chloride channels. Nat. Genet. 4:426-431.