PUBLICATION
Visualization, characterization and modulation of calcium signaling during the development of slow muscle cells in intact zebrafish embryos
- Authors
- Cheung, C.Y., Webb, S.E., Love, D.R., and Miller, A.L.
- ID
- ZDB-PUB-110523-16
- Date
- 2011
- Source
- The International journal of developmental biology 55(2): 153-74 (Journal)
- Registered Authors
- Cheung, Yuk Kam Chris, Love, Donald R., Miller, Andrew L., Webb, Sarah E.
- Keywords
- Ca2+ imaging, IP3R, RyR, slow muscle cell, transgenic zebrafish
- MeSH Terms
-
- Animals
- Calcium/metabolism
- Zebrafish/embryology*
- Zebrafish/metabolism*
- Actins/metabolism
- Recombinant Proteins/biosynthesis
- Recombinant Proteins/genetics
- Animals, Genetically Modified
- Inositol 1,4,5-Trisphosphate Receptors/metabolism
- Nifedipine/pharmacology
- Aequorin/biosynthesis
- Aequorin/genetics
- Bungarotoxins/pharmacology
- Calcium Signaling*
- Ryanodine Receptor Calcium Release Channel/metabolism
- Muscle Development/drug effects
- Muscle Development/physiology*
- Muscle, Skeletal/drug effects
- Muscle, Skeletal/embryology*
- Muscle, Skeletal/metabolism
- Myosins/metabolism
- Apoproteins/biosynthesis
- Apoproteins/genetics
- Veratrum Alkaloids/pharmacology
- Colforsin/pharmacology
- PubMed
- 21553383 Full text @ Int. J. Dev. Biol.
Citation
Cheung, C.Y., Webb, S.E., Love, D.R., and Miller, A.L. (2011) Visualization, characterization and modulation of calcium signaling during the development of slow muscle cells in intact zebrafish embryos. The International journal of developmental biology. 55(2):153-74.
Abstract
Intact zebrafish embryos were used as an in vivo animal model to investigate the role of Ca(2+) signaling during the differentiation of slow muscle cells (SMCs) within forming skeletal muscle. Transgenic zebrafish were generated using an a-actin promoter that targeted apoaequorin expression specifically to muscle cells. Two distinct Ca(2+) signaling periods (CSPs) were visualized in the developing SMCs: between ~17.5-19.5 hours post-fertilization (hpf) and after ~23 hpf, separated by a ~3.5 h Ca(2+) signaling quiet period. Further spatial characterization of these Ca2+ signals using confocal fluorescent microscopy and calcium green-1 dextran as a reporter, indicated that the earlier CSP displayed distinct nuclear and cytoplasmic components, whereas the later CSP was predominantly cytoplasmic. Both CSPs consisted of a series of oscillating Ca(2+) waves generated at distinct frequencies, while the earlier CSP also displayed a slow rise then fall in the Ca(2+) baseline-level. Imaging of cyclopamine- and forskolin-treated wild-type, or smo(-/-) mutant embryos, where SMCs do not form, confirmed the specific cell population generating the signals. Treating embryos with antagonists indicated that both IP(3)Rs and RyRs are responsible for generating the temporal characteristics of the Ca(2+) signaling signature, and that the latter plays a necessary role in SMC differentiation and subsequent myotome patterning. Together, these data support and extend the proposition that specific spatiotemporal patterns of spontaneous Ca(2+) signals might be used for different as well as combinatorial regulation of both nuclear and cytosolic signal transduction cascades, resulting in myofibrillogenesis in SMCs as well as myotome patterning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping