PUBLICATION
Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish
- Authors
- Geudens, I., Herpers, R., Hermans, K., Segura, I., Ruiz de Almodovar, C., Bussmann, J., De Smet, F., Vandevelde, W., Hogan, B.M., Siekmann, A., Claes, F., Moore, J.C., Pistocchi, A.S., Loges, S., Mazzone, M., Mariggi, G., Bruyere, F., Cotelli, F., Kerjaschki, D., Noel, A., Foidart, J.M., Gerhardt, H., Ny, A., Langenberg, T., Lawson, N.D., Duckers, H.J., Schulte-Merker, S., Carmeliet, P., and Dewerchin, M.
- ID
- ZDB-PUB-101213-1
- Date
- 2010
- Source
- Arterioscler. Thromb. Vasc. Biol. 30(9): 1695-1702 (Journal)
- Registered Authors
- Bussmann, Jeroen, Cotelli, Franco, Herpers, Robert, Hogan, Ben M., Langenberg, Tobias, Lawson, Nathan, Moore, John, Schulte-Merker, Stefan, Siekmann, Arndt Friedrich
- Keywords
- vascular biology, gene silencing, lymphangiogenesis, notch signaling, zebrafish
- MeSH Terms
-
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental
- Intracellular Signaling Peptides and Proteins
- Cell Movement
- Luminescent Proteins/biosynthesis
- Luminescent Proteins/genetics
- Receptors, Notch/genetics
- Receptors, Notch/metabolism*
- Lymphatic System/embryology
- Lymphatic System/metabolism*
- Signal Transduction*
- Animals, Genetically Modified
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Chlorocebus aethiops
- Animals
- Humans
- Coculture Techniques
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- COS Cells
- Cell Proliferation
- Gene Knockdown Techniques
- Thoracic Duct/embryology
- Thoracic Duct/metabolism
- Gene Silencing
- Endothelial Cells/metabolism
- RNA, Messenger/metabolism
- Biomarkers/metabolism
- Lymphangiogenesis*/genetics
- PubMed
- 20466977 Full text @ Arterioscler. Thromb. Vasc. Biol.
Citation
Geudens, I., Herpers, R., Hermans, K., Segura, I., Ruiz de Almodovar, C., Bussmann, J., De Smet, F., Vandevelde, W., Hogan, B.M., Siekmann, A., Claes, F., Moore, J.C., Pistocchi, A.S., Loges, S., Mazzone, M., Mariggi, G., Bruyere, F., Cotelli, F., Kerjaschki, D., Noel, A., Foidart, J.M., Gerhardt, H., Ny, A., Langenberg, T., Lawson, N.D., Duckers, H.J., Schulte-Merker, S., Carmeliet, P., and Dewerchin, M. (2010) Role of delta-like-4/Notch in the formation and wiring of the lymphatic network in zebrafish. Arterioscler. Thromb. Vasc. Biol.. 30(9):1695-1702.
Abstract
OBJECTIVE: To study whether Notch signaling, which regulates cell fate decisions and vessel morphogenesis, controls lymphatic development.
METHODS AND RESULTS: In zebrafish embryos, sprouts from the axial vein have lymphangiogenic potential because they give rise to the first lymphatics. Knockdown of delta-like-4 (Dll4) or its receptors Notch-1b or Notch-6 in zebrafish impaired lymphangiogenesis. Dll4/Notch silencing reduced the number of sprouts producing the string of parchordal lymphangioblasts; instead, sprouts connecting to the intersomitic vessels were formed. At a later phase, Notch silencing impaired navigation of lymphatic intersomitic vessels along their arterial templates.
CONCLUSIONS: These studies imply critical roles for Notch signaling in the formation and wiring of the lymphatic network.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping