ZFIN ID: ZDB-PERS-970512-19
Köster, Reinhard W.
Email: r.koester@tu-bs.de
URL: http://www.zoologie.tu-bs.de/index.php/de/zellulaere-molekulare-neurobiologie
Affiliation: Köster Lab
Address: Technische Universität Braunschweig Institute of Zoology Division of Cellular and Molecular Neurobiology Spielmannstrasse 7 Braunschweig, 38106 Germany
Country: Germany
Phone: +49-531-391-3230
Fax: +49 (0)531 391 3222

Full Professor for Cellular and Molecular Neurobiology
Institute of Zoology
Technische Universität Braunschweig

Associate Professor for Cell Biology and Cell Physiology
Institute of Zoology
Technische Universität Braunschweig

Group Leader/PI of Zebrafish Neuroimaging Group 2003 - 2010
Institute for Developmental Genetics
Helmholtz Zentrum München in Neuherberg/Munich

Postdoctoral Research 1998 - 2003
Biological Imaging Center
California Institute of Technology, Pasadena, USA

Ph.D. Thesis 1995 - 1998
Department of Molecular Developmental Genetics
Max-Planck-Institute of Biophysical Chemistry, Göttingen

Diploma Thesis 1994
Dept. of Physiological Chemistry and Department of Neurology
Julius-Maximilians-University of Würzburg

Study of Organic, Inorganic, 1989-1994
Physical Chemistry and Biochemistry
Julius-Maximilians-University of Würzburg

The Zebrafish Neuroimaging Group aims to understand the molecular mechanisms that govern the migration of neuronal precursors cells in the developing central nervous system. Our focus is on the investigation of the differentiation of the zebrafish cerebellum.

We try to elucidate how migrating neuronal precursors initiate migration, interact with other cells, make pathfinding decisions, stop migration and adopt a specific fate.

As migratory processes are very dependent on their environment cellular behavior is ideally observed directly in vivo. Thus, we employ intravital imaging methods that allow neuronal precursors to be observed continuously as they move, interact with neighbors and respond to altered genetic conditions.

In addition, we are establishing zebrafish models for neurodegenerative diseases of the cerebellum. These models are accessible for in vivo imaging studies and behavioral analysis.

Lindemann, N., Kalix, L., Possiel, J., Stasch, R., Kusian, T., Köster, R.W., von Trotha, J.W. (2022) A comparative analysis of Danionella cerebrum and zebrafish (Danio rerio) larval locomotor activity in a light-dark test. Frontiers in behavioral neuroscience. 16:885775
Papadaki, S., Wang, X., Wang, Y., Zhang, H., Jia, S., Liu, S., Yang, M., Zhang, D., Jia, J.M., Köster, R.W., Namikawa, K., Piatkevich, K.D. (2022) Dual-expression system for blue fluorescent protein optimization. Scientific Reports. 12:10190
Elsaey, M.A., Namikawa, K., Köster, R.W. (2021) Genetic Modeling of the Neurodegenerative Disease Spinocerebellar Ataxia Type 1 in Zebrafish. International Journal of Molecular Sciences. 22(14):
Buchberger, A., Schepergerdes, L., Flaßhoff, M., Kunick, C., Köster, R.W. (2021) A novel inhibitor rescues cerebellar defects in a zebrafish model of Down Syndrome-associated kinase Dyrk1A overexpression. The Journal of biological chemistry. 297(1):100853
Schramm, P., Hetsch, F., Meier, J.C., Köster, R.W. (2021) In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal. Journal of visualized experiments : JoVE. (168):
Theisen, U., Ernst, A.U., Heyne, R.L.S., Ring, T.P., Thorn-Seshold, O., Köster, R.W. (2020) Microtubules and motor proteins support zebrafish neuronal migration by directing cargo. The Journal of cell biology. 219(10):
Vauti, F., Stegemann, L.A., Vögele, V., Köster, R.W. (2020) All-age whole mount in situ hybridization to reveal larval and juvenile expression patterns in zebrafish. PLoS One. 15:e0237167
Chang, W., Pedroni, A., Hohendorf, V., Giacomello, S., Hibi, M., Köster, R.W., Ampatzis, K. (2020) Functionally distinct Purkinje cell types show temporal precision in encoding locomotion. Proceedings of the National Academy of Sciences of the United States of America. 117(29):17330-17337
Mattern, K., von Trotha, J.W., Erfle, P., Köster, R.W., Dietzel, A. (2020) NeuroExaminer: an all-glass microfluidic device for whole-brain in vivo imaging in zebrafish. Communications biology. 3:311
Li, J., Ünal, C.M., Namikawa, K., Steinert, M., Köster, R.W. (2020) Development of a Larval Zebrafish Infection Model for Clostridioides difficile. Journal of visualized experiments : JoVE. (156):
Namikawa, K., Dorigo, A., Köster, R.W. (2019) Neurological Disease Modelling for Spinocerebellar Ataxia Using Zebrafish. Journal of experimental neuroscience. 13:1179069519880515
Plate, J., Sassen, W.A., Hassan, A.H., Lehne, F., Köster, R.W., Kruse, T. (2019) S-Sulfocysteine Induces Seizure-Like Behaviors in Zebrafish. Frontiers in pharmacology. 10:122
Jagau, H., Behrens, I.K., Lahme, K., Lorz, G., Köster, R.W., Schneppenheim, R., Obser, T., Brehm, M.A., König, G., Kohler, T.P., Rohde, M., Frank, R., Tegge, W., Fulde, M., Hammerschmidt, S., Steinert, M., Bergmann, S. (2019) Von Willebrand Factor Mediates Pneumococcal Aggregation and Adhesion in Blood Flow. Frontiers in microbiology. 10:511
Namikawa, K., Dorigo, A., Zagrebelsky, M., Russo, G., Kirmann, T., Fahr, W., Dübel, S., Korte, M., Köster, R.W. (2019) Modeling neurodegenerative Spinocerebellar Ataxia type 13 in zebrafish using a Purkinje neuron specific tunable co-expression system. The Journal of neuroscience : the official journal of the Society for Neuroscience. 39(20):3948-3969
Russo, G., Lehne, F., Pose Méndez, S.M., Dübel, S., Köster, R.W., Sassen, W.A. (2018) Culture and Transfection of Zebrafish Primary Cells. Journal of visualized experiments : JoVE. (138):
Theisen, U., Hey, S., Hennig, C.D., Schnabel, R., Köster, R.W. (2018) Glycine is able to induce both a motility speed in- and decrease during zebrafish neuronal migration. Communicative & integrative biology. 11:1-7
Theisen, U., Hennig, C., Ring, T., Schnabel, R., Köster, R.W. (2018) Neurotransmitter-mediated activity spatially controls neuronal migration in the zebrafish cerebellum. PLoS Biology. 16:e2002226
Russo, G., Theisen, U., Fahr, W., Helmsing, S., Hust, M., Köster, R.W., Dübel, S. (2017) Sequence defined antibodies improve the detection of Cadherin 2 (N-Cadherin) during Zebrafish development. New biotechnology. 45:98-112
Sassen, W.A., Lehne, F., Russo, G., Wargenau, S., Dübel, S., Köster, R.W. (2017) Embryonic zebrafish primary cell culture for transfection and live cellular and subcellular imaging. Developmental Biology. 430(1):18-31
Weber, T., Namikawa, K., Winter, B., Müller-Brown, K., Kühn, R., Wurst, W., Köster, R.W. (2016) Caspase-mediated apoptosis induction in zebrafish cerebellar Purkinje neurons. Development (Cambridge, England). 143(22):4279-4287
Biechl, D., Dorigo, A., Köster, R.W., Grothe, B., Wullimann, M.F. (2016) Eppur Si Muove: Evidence for an External Granular Layer and Possibly Transit Amplification in the Teleostean Cerebellum. Frontiers in Neuroanatomy. 10:49
Matsui, H., Dorigo, A., Buchberger, A., Hocking, J.C., Distel, M., Köster, R.W. (2015) A zebrafish jam-b2 Gal4-enhancer trap line recapitulates endogenous jam-b2 expression in extraocular muscles. Developmental dynamics : an official publication of the American Association of Anatomists. 244(12):1574-80
Matsui, H., Namikawa, K., Köster, R.W. (2014) Identification of the zebrafish red nucleus using Wheat Germ Agglutinin transneuronal tracing. Communicative & integrative biology. 7:e994383
Schoetz, U., Deliolanis, N.C., Ng, D., Pauli, J., Resch-Genger, U., Kühn, E., Heuer, S., Beisker, W., Köster, R.W., Zitzelsberger, H., Caldwell, R.B. (2014) Usefulness of a Darwinian System in a Biotechnological Application: Evolution of Optical Window Fluorescent Protein Variants under Selective Pressure. PLoS One. 9:e107069
Matsui, H., Namikawa, K., Babaryka, A., Köster, R.W. (2014) Functional regionalization of the teleost cerebellum analyzed in vivo. Proceedings of the National Academy of Sciences of the United States of America. 111(32):11846-51
Flinn, L.J., Keatinge, M., Bretaud, S., Mortiboys, H., Matsui, H., De Felice, E., Woodroof, H.I., Brown, L., McTighe, A., Soellner, R., Allen, C.E., Heath, P.R., Milo, M., Muqit, M.M., Reichert, A.S., Köster, R.W., Ingham, P.W., and Bandmann, O. (2013) TigarB causes mitochondrial dysfunction and neuronal loss in PINK1 deficiency. Annals of neurology. 74(6):837-47
Weber, T., and Köster, R. (2013) Genetic tools for multicolor imaging in zebrafish larvae. Methods (San Diego, Calif.). 62(3):279-91
Hocking, J.C., Distel, M., and Köster, R.W. (2013) Studying cellular and subcellular dynamics in the developing zebrafish nervous system. Experimental neurology. 242:1-10
Xie, X., Mathias, J.R., Smith, M.A., Walker, S.L., Teng, Y., Distel, M., Koster, R.W., Sirotkin, H.I., Saxena, M.T., and Mumm, J.S. (2012) Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression in a NRSF/REST-dependent manner. BMC Biology. 10:93
Sasmal, P.K., Carregal-Romero, S., Han, A.A., Streu, C.N., Lin, Z., Namikawa, K., Elliott, S.L., Köster, R.W., Parak, W.J., and Meggers, E. (2012) Catalytic Azide Reduction in Biological Environments. Chembiochem : a European journal of chemical biology. 13(8):1116-1120
Oberoi, T.K., Dogan, T., Hocking, J.C., Scholz, R.P., Mooz, J., Anderson, C.L., Karreman, C., Heringdorf, D., Schmidt, G., Ruonala, M., Namikawa, K., Harms, G.S., Carpy, A., Macek, B., Köster, R.W., and Rajalingam, K. (2012) IAPs regulate the plasticity of cell migration by directly targeting Rac1 for degradation. The EMBO journal. 31(1):14-28
Walker, S.L., Ariga, J., Mathias, J.R., Coothankandaswamy, V., Xie, X., Distel, M., Köster, R.W., Parsons, M.J., Bhalla, K.N., Saxena, M.T., and Mumm, J.S. (2012) Automated reporter quantification in vivo: high-throughput screening method for reporter-based assays in zebrafish. PLoS One. 7(1):e29916
Hoegler, K.J., Distel, M., Köster, R.W., and Horne, J.H. (2011) Targeting Olfactory Bulb Neurons Using Combined In Vivo Electroporation and Gal4-Based Enhancer Trap Zebrafish Lines. Journal of visualized experiments : JoVE. (54)
Distel, M., Jennifer, C.H., and Köster, R.W. (2011) In vivo cell biology using Gal4-mediated multicolor subcellular labeling in zebrafish. Communicative & integrative biology. 4(3):336-9
Wullimann, M.F., Mueller, T., Distel, M., Babaryka, A., Grothe, B., and Köster, R.W. (2011) The long adventurous journey of rhombic lip cells in jawed vertebrates: a comparative developmental analysis. Frontiers in Neuroanatomy. 5:27
Santoriello, C., Gennaro, E., Anelli, V., Distel, M., Kelly, A., Köster, R.W., Hurlstone, A., and Mione, M. (2010) Kita Driven Expression of Oncogenic HRAS Leads to Early Onset and Highly Penetrant Melanoma in Zebrafish. PLoS One. 5(12):e15170
Distel, M., Hocking, J.C., Volkmann, K., and Köster, R.W. (2010) The centrosome neither persistently leads migration nor determines the site of axonogenesis in migrating neurons in vivo. The Journal of cell biology. 191(4):875-890
Volkmann, K., Chen, Y.Y., Harris, M.P., Wullimann, M.F., and Köster, R.W. (2010) The zebrafish cerebellar upper rhombic lip generates tegmental hindbrain nuclei by long-distance migration in an evolutionary conserved manner. The Journal of comparative neurology. 518(14):2794-2817
Kühn, E., and Köster, R.W. (2010) Analysis of gene expression by in situ hybridization on adult zebrafish brain sections. CSH protocols. 2010(2):pdb.prot5382
Anelli, V., Santoriello, C., Distel, M., Köster, R.W., Ciccarelli, F.D., and Mione, M. (2009) Global repression of cancer gene expression in a zebrafish model of melanoma is linked to epigenetic regulation. Zebrafish. 6(4):417-424
Rieger, S., Senghaas, N., Walch, A., and Köster, R.W. (2009) Cadherin-2 controls directional chain migration of cerebellar granule neurons. PLoS Biology. 7(11):e1000240
Distel, M., Wullimann, M.F., and Köster, R.W. (2009) Optimized Gal4 genetics for permanent gene expression mapping in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 106(32):13365-13370
Nikolaou, N., Watanabe-Asaka, T., Gerety, S., Distel, M., Köster, R.W., and Wilkinson, D.G. (2009) Lunatic fringe promotes the lateral inhibition of neurogenesis. Development (Cambridge, England). 136(15):2523-2533
Senghaas, N., and Köster, R.W. (2009) Culturing and Transfecting Zebrafish PAC2 Fibroblast Cells. CSH protocols. 2009(6):pdb.prot5235
Flinn, L., Mortiboys, H., Volkmann, K., Köster, R.W., Ingham, P.W., and Bandmann, O. (2009) Complex I deficiency and dopaminergic neuronal cell loss in parkin-deficient zebrafish (Danio rerio). Brain : a journal of neurology. 132(Pt 6):1613-1623
Paquet, D., Bhat, R., Sydow, A., Mandelkow, E.M., Berg, S., Hellberg, S., Fälting, J., Distel, M., Köster, R.W., Schmid, B., and Haass, C. (2009) A zebrafish model of tauopathy allows in vivo imaging of neuronal cell death and drug evaluation. J. Clin. Invest.. 119(5):1382-1395
Babaryka, A., Kuhn, E., and Koster, R.W. (2009) In vivo synthesis of meganuclease for generating transgenic zebrafish Danio rerio. Journal of Fish Biology. 74(2):452-457
Chu, Y., Senghaas, N., Köster, R.W., Wurst, W., and Kühn, R. (2008) Novel caspase-suicide proteins for tamoxifen-inducible apoptosis. Genesis (New York, N.Y. : 2000). 46(10):530-536
Rieger, S., Volkmann, K., and Köster, R.W. (2008) Polysialyltransferase expression is linked to neuronal migration in the developing and adult zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 237(1):276-285
Volkmann, K., Rieger, S., Babaryka, A., and Köster, R.W. (2008) The zebrafish cerebellar rhombic lip is spatially patterned in producing granule cell populations of different functional compartments. Developmental Biology. 313(1):167-180
Koster, R.W., and Fraser, S.E. (2006) FGF signaling mediates regeneration of the differentiating cerebellum through repatterning of the anterior hindbrain and reinitiation of neuronal migration. The Journal of neuroscience : the official journal of the Society for Neuroscience. 26(27):7293-7304
Distel, M., Babaryka, A., Köster, R.W. (2006) Multicolor in vivo time-lapse imaging at cellular resolution by stereomicroscopy. Developmental dynamics : an official publication of the American Association of Anatomists. 235(3):843-845
Rieger, S., Kulkarni, R.P., Darcy, D., Fraser, S.E., and Köster, R.W. (2005) Quantum dots are powerful multipurpose vital labeling agents in zebrafish embryos. Developmental dynamics : an official publication of the American Association of Anatomists. 234(3):670-681
Köster, R.W., and Fraser, S.E. (2004) Time-lapse microscopy of brain development. The Zebrafish: Cellular and Developmental Biology,2nd Ed. Methods Cell Biol.. 76:207-235
Hove, J.R., Köster, R.W., Forouhar, A.S., Acevedo-Bolton, G., Fraser, S.E., and Gharib, M. (2003) Intracardiac fluid forces are an essential epigenetic factor for embryonic cardiogenesis. Nature. 421(6919):172-177
Wagner, T.U., Renn, J., Riemensperger, T., Volff, J.N., Köster, R.W., Goerlich, R., Schartl, M., and Winkler, C. (2003) The teleost fish medaka (Oryzias latipes) as genetic model to study gravity dependent bone homeostasis in vivo. Advances in space research : the official journal of the Committee on Space Research (COSPAR). 32(8):1459-1465
Köster, R.W. and Fraser, S.E. (2001) Direct imaging of in vivo neuronal migration in the developing cerebellum. Current biology : CB. 11(23):1858-1863
Köster, R.W. and Fraser, S.E. (2001) Tracing transgene expression in living zebrafish embryos. Developmental Biology. 233(2):329-346
Udvadia, A.J., Köster, R.W., and Skene, J.H. (2001) GAP-43 promoter elements in transgenic zebrafish reveal a difference in signals for axon growth during CNS development and regeneration. Development (Cambridge, England). 128(7):1175-1182
Götz, R., Köster, R.W., Winkler, C., Raulf, F., Lottspeich, F., Schartl, M., and Thoenen, H. (1994) Neurotrophin-6 is a new member of the nerve growth factor family. Nature. 372(6503):266-269

Loosli, F., K�ster, R.W., Carl, M., Kuhnlein, R., Henrich, T., Mucke, M., Krone, A., and Wittbrodt, J. (2000) A genetic screen for mutations affecting embryonic development in medaka fish (Oryzias latipes). Mech. Dev.. 97(1-2):133-139

K�ster, R.W., Kuhnlein, R.P., and Wittbrodt, J. (2000) Ectopic Sox3 activity elicits sensory placode formation. Mech. Dev. 95(1-2):175-187

Loosli, F., K�ster, R.W., Carl, M., Krone, A., and Wittbrodt, J. (1998) Six3, a medaka homologue of the Drosophila homeobox gene sine oculis is expressed in the anterior embryonic shield and the developing eye. Mech. Dev.. 74(1-2):159-164

K�ster, R.W., Stick, R., Loosli, F., and Wittbrodt, J. (1997) Medaka spalt acts as a target gene of hedgehog signaling. Development. 124(16):3147-3156

R. K�ster, R. G�tz, J. Altschmied, M. Sendtner, M. Schartl (1996). Comparison of monocistronic and bicistronic constructs for neurotrophin transgene and reporter gene expression in fish cells. Molecular Marine Biology and Biotechnology 5: 1-8.

Oliver, G., Loosli, F., K�ster, R.W., Wittbrodt, J., and Gruss, P. (1996) Ectopic lens induction in fish in response to the murine homeobox gene Six3. Mech. Dev.. 60(2):233-239