PUBLICATION

Zebrafish wnt3 is expressed in developing neural tissue

Authors
Clements, W.K., Ong, K.G., and Traver, D.
ID
ZDB-PUB-090526-20
Date
2009
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   238(7): 1788-1795 (Journal)
Registered Authors
Clements, Wilson, Traver, David
Keywords
zebrafish, wnt3, wnt3l, wnt3a, neuron, neuronal, nervous system, CNS, neural patterning, ZLI, zona limitans, tectum, Wnt pathway, Wnt signaling, canonical Wnt signaling
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Body Patterning/genetics
  • Cloning, Molecular
  • Embryo, Nonmammalian
  • Molecular Sequence Data
  • Nerve Net/embryology
  • Nerve Net/metabolism
  • Nervous System/embryology*
  • Nervous System/metabolism*
  • Neurons/metabolism
  • Neurons/physiology
  • Phylogeny
  • Sequence Homology, Amino Acid
  • Signal Transduction/genetics
  • Signal Transduction/physiology
  • Wnt Proteins/genetics*
  • Wnt Proteins/metabolism
  • Wnt Proteins/physiology
  • Wnt3 Protein
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed
19452545 Full text @ Dev. Dyn.
Abstract
Wnt signaling regulates embryonic patterning and controls stem cell homeostasis, while aberrant Wnt activity is associated with disease. One Wnt family member, Wnt3, is required in mouse for specification of mesoderm, and later regulates neural patterning, apical ectodermal ridge formation, and hair growth. We have identified and performed preliminary characterization of the zebrafish wnt3 gene. wnt3 is expressed in the developing tailbud and neural tissue including the zona limitans intrathalamica (ZLI), optic tectum, midbrain-hindbrain boundary, and dorsal hindbrain and spinal cord. Expression in these regions suggests that Wnt3 participates in processes such as forebrain compartmentalization and regulation of tectal wiring topography by retinal ganglia axons. Surprisingly, wnt3 expression is not detectable during mesoderm specification, making it unlikely that Wnt3 regulates this process in zebrafish. This lack of early expression should make it possible to study later Wnt3-regulated patterning events, such as neural patterning, by knockdown studies in zebrafish.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping