PUBLICATION

Apical membrane maturation and cellular rosette formation during morphogenesis of the zebrafish lateral line

Authors
Hava, D., Forster, U., Matsuda, M., Cui, S., Link, B.A., Eichhorst, J., Wiesner, B., Chitnis, A., and Abdelilah-Seyfried, S.
ID
ZDB-PUB-090217-29
Date
2009
Source
Journal of Cell Science   122(Pt 5): 687-695 (Journal)
Registered Authors
Abdelilah-Seyfried, Salim, Chitnis, Ajay, Link, Brian
Keywords
none
MeSH Terms
  • Animals
  • Cell Adhesion/physiology*
  • Cell Polarity
  • Cell Shape
  • Intracellular Signaling Peptides and Proteins
  • Lateral Line System*/cytology
  • Lateral Line System*/embryology
  • Membrane Proteins/genetics
  • Membrane Proteins/metabolism
  • Morphogenesis*
  • Protein Kinase C/metabolism
  • Receptors, Notch/genetics
  • Receptors, Notch/metabolism
  • Signal Transduction/physiology
  • Stem Cells/cytology
  • Stem Cells/physiology
  • Zebrafish*/anatomy & histology
  • Zebrafish*/embryology
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
19208766 Full text @ J. Cell Sci.
Abstract
Tissue morphogenesis and cell sorting are major forces during organ development. Here, we characterize the process of tissue morphogenesis within the zebrafish lateral line primordium, a migratory sheet of cells that gives rise to the neuromasts of the posterior lateral line organ. We find that cells within this epithelial tissue constrict actin-rich membranes and enrich apical junction proteins at apical focal points. The coordinated apical membrane constriction in single Delta D-positive hair cell progenitors and in their neighbouring prospective support cells generates cellular rosettes. Live imaging reveals that cellular rosettes subsequently separate from each other and give rise to individual neuromasts. Genetic analysis uncovers an involvement of Lethal giant larvae proteins in the maturation of apical junction belts during cellular rosette formation. Our findings suggest that apical constriction of cell membranes spatially confines regions of strong cell-cell adhesion and restricts the number of tightly interconnected cells into cellular rosettes, which ensures the correct deposition of neuromasts during morphogenesis of the posterior lateral line organ.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes