ZFIN ID: ZDB-PERS-980210-43
Link, Brian
Email: blink@mcw.edu
URL: http://www.mcw.edu/cellbiology/brianlinkphd.htm
Affiliation: Link Lab
Address: Department of Cell Biology, Neurobiology, and Anatomy Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, WI 53226-0509 USA
Country: United States
Phone: (414) 456-8072
Fax: (414) 456-6517

We are using genetics and live cell imaging to study ocular development and disease. In particular, we are interested in retinal neurogenesis and how cell type diversity and laminar position is established. For disease studies we are focused on the complex gene interactions and cell biological basis of glaucoma-associated phenotypes. These include regulation of intraocular pressure and ganglion cell degeneration.

Brandt, Z.J., Collery, R.F., Besharse, J.C., Link, B.A. (2021) Ablation of mpeg+ Macrophages Exacerbates mfrp-Related Hyperopia. Investigative ophthalmology & visual science. 62:13
Clark, B.S., Miesfeld, J.B., Flinn, M.A., Collery, R.F., Link, B.A. (2021) Dynamic Polarization of Rab11a Modulates Crb2a Localization and Impacts Signaling to Regulate Retinal Neurogenesis. Frontiers in cell and developmental biology. 8:608112
Clark, E.M., Link, B.A. (2021) Complementary and divergent functions of zebrafish Tango1 and Ctage5 in tissue development and homeostasis. Molecular biology of the cell. 32(5):391-401
Flinn, M.A., Otten, C., Brandt, Z.J., Bostrom, J.R., Kenarsary, A., Wan, T.C., Auchampach, J.A., Abdelilah-Seyfried, S., O'Meara, C.C., Link, B.A. (2020) Llgl1 regulates zebrafish cardiac development by mediating Yap stability in cardiomyocytes. Development (Cambridge, England). 147(16):
Brandt, Z.J., Echert, A.E., Bostrom, J.R., North, P.N., Link, B.A. (2020) Core Hippo pathway components act as a brake on Yap/Taz in the development and maintenance of the biliary network. Development (Cambridge, England). 147(12):
Clark, E.M., Nonarath, H.J.T., Bostrom, J.R., Link, B.A. (2019) Establishment and validation of an endoplasmic reticulum stress reporter to monitor zebrafish ATF6 activity in development and disease. Disease models & mechanisms. 13(1):
Brandt, Z.J., North, P.N., Link, B.A. (2019) Somatic Mutations of lats2 Cause Peripheral Nerve Sheath Tumors in Zebrafish. Cells. 8(9)
Collery, R.F., Link, B.A. (2019) Precise Short Sequence Insertion in Zebrafish Using a CRISPR/Cas9 Approach to Generate a Constitutively Soluble Lrp2 Protein. Frontiers in cell and developmental biology. 7:167
Hanovice, N.J., Leach, L.L., Slater, K., Gabriel, A.E., Romanovicz, D., Shao, E., Collery, R., Burton, E.A., Lathrop, K.L., Link, B.A., Gross, J.M. (2019) Regeneration of the zebrafish retinal pigment epithelium after widespread genetic ablation. PLoS Genetics. 15:e1007939
Rausch, V., Bostrom, J.R., Park, J., Bravo, I.R., Feng, Y., Hay, D.C., Link, B.A., Hansen, C.G. (2018) The Hippo Pathway Regulates Caveolae Expression and Mediates Flow Response via Caveolae. Current biology : CB. 29(2):242-255.e6
Lewis, T.R., Kundinger, S.R., Link, B.A., Insinna, C., Besharse, J.C. (2018) Kif17 phosphorylation regulates photoreceptor outer segment turnover. BMC Cell Biology. 19:25
Cox, A.G., Tsomides, A., Yimlamai, D., Hwang, K.L., Miesfeld, J., Galli, G.G., Fowl, B.H., Fort, M., Ma, K.Y., Sullivan, M.R., Hosios, A.M., Snay, E., Yuan, M., Brown, K.K., Lien, E.C., Chhangawala, S., Steinhauser, M.L., Asara, J.M., Houvras, Y., Link, B., Vander Heiden, M.G., Camargo, F.D., Goessling, W. (2018) Yap regulates glucose utilization and sustains nucleotide synthesis to enable organ growth. The EMBO journal. 37(22):
Flinn, M.A., Jeffery, B.E., O'Meara, C.C., Link, B.A. (2018) Yap is Required for Scar Formation but not Myocyte Proliferation During Heart Regeneration in Zebrafish. Cardiovascular research. 115(3):570-577
Lapierre-Landry, M., Huckenpahler, A.L., Link, B.A., Collery, R.F., Carroll, J., Skala, M.C. (2018) Imaging Melanin Distribution in the Zebrafish Retina Using Photothermal Optical Coherence Tomography. Translational vision science & technology. 7:4
Prokop, J.W., Yeo, N.C., Ottmann, C., Chhetri, S.B., Florus, K.L., Ross, E.J., Sosonkina, N., Link, B.A., Freedman, B.I., Coppola, C.J., McDermott-Roe, C., Leysen, S., Milroy, L.G., Meijer, F.A., Geurts, A.M., Rauscher, F.J., Ramaker, R., Flister, M.J., Jacob, H.J., Mendenhall, E.M., Lazar, J. (2018) Characterization of Coding/Noncoding Variants for SHROOM3 in Patients with CKD. Journal of the American Society of Nephrology : JASN. 29:1525-1535
Hiscock, T.W., Miesfeld, J.B., Mosaliganti, K.R., Link, B.A., Megason, S.G. (2018) Feedback between tissue packing and neurogenesis in the zebrafish neural tube. Development (Cambridge, England). 145(9)
Hendee, K.E., Sorokina, E.A., Muheisen, S.S., Reis, L.M., Tyler, R.C., Markovic, V., Cuturilo, G., Link, B.A., Semina, E.V. (2018) PITX2 deficiency and associated human disease: insights from the zebrafish model. Human molecular genetics. 27(10):1675-1695
Huckenpahler, A., Wilk, M., Link, B., Carroll, J., Collery, R. (2018) Repeatability and Reproducibility of In Vivo Cone Density Measurements in the Adult Zebrafish Retina. Advances in experimental medicine and biology. 1074:151-156
Lewis, T.R., Zareba, M., Link, B.A., Besharse, J.C. (2017) Cone myoid elongation involves unidirectional microtubule movement mediated by dynein-1. Molecular biology of the cell. 29(2):180-190
Lewis, T.R., Kundinger, S.R., Pavlovich, A.L., Bostrom, J.R., Link, B.A., Besharse, J.C. (2017) Cos2/Kif7 and OSM-3/Kif17 Regulate Onset of Outer Segment Development in Zebrafish Photoreceptors Through Distinct Mechanisms. Developmental Biology. 425(2):176-190
Wilk, M.A., Huckenpahler, A.L., Collery, R.F., Link, B.A., Carroll, J. (2017) The Effect of Retinal Melanin on Optical Coherence Tomography Images. Translational vision science & technology. 6:8
Collery, R.F., Volberding, P.J., Bostrom, J.R., Link, B.A., Besharse, J.C. (2016) Loss of Zebrafish Mfrp Causes Nanophthalmia, Hyperopia, and Accumulation of Subretinal Macrophages. Investigative ophthalmology & visual science. 57:6805-6814
Hilinski, W.C., Bostrom, J.R., England, S.J., Juárez-Morales, J.L., de Jager, S., Armant, O., Legradi, J., Strähle, U., Link, B.A., Lewis, K.E. (2016) Lmx1b is required for the glutamatergic fates of a subset of spinal cord neurons. Neural Development. 11:16
Huckenpahler, A.L., Wilk, M.A., Cooper, R.F., Moehring, F., Link, B.A., Carroll, J., Collery, R.F. (2016) Imaging the adult zebrafish cone mosaic using optical coherence tomography. Visual neuroscience. 33:E011
Miesfeld, J.B., Gestri, G., Clark, B.S., Flinn, M.A., Poole, R.J., Bader, J.R., Besharse, J.C., Wilson, S.W., Link, B.A. (2015) Yap and Taz regulate retinal pigment epithelial cell fate. Development (Cambridge, England). 142(17):3021-32
Yeo, N.C., O'Meara, C.C., Bonomo, J.A., Veth, K.N., Tomar, R., Flister, M.J., Drummond, I.A., Bowden, D.W., Freedman, B.I., Lazar, J., Link, B.A., Jacob, H.J. (2015) Shroom3 contributes to the maintenance of the glomerular filtration barrier integrity. Genome research. 25(1):57-65
Collery, R.F., Veth, K.N., Dubis, A.M., Carroll, J., Link, B.A. (2014) Rapid, Accurate, and Non-Invasive Measurement of Zebrafish Axial Length and Other Eye Dimensions Using SD-OCT Allows Longitudinal Analysis of Myopia and Emmetropization. PLoS One. 9:e110699
Paulus, J.D., Link, B.A. (2014) Loss of Optineurin In Vivo Results in Elevated Cell Death and Alters Axonal Trafficking Dynamics. PLoS One. 9:e109922
Miesfeld, J.B., Link, B.A. (2014) Establishment of transgenic lines to monitor and manipulate Yap/Taz-Tead activity in zebrafish reveals both evolutionarily conserved and divergent functions of the Hippo pathway. Mechanisms of Development. 133:177-88
Buckley, C.E., Ren, X., Ward, L.C., Girdler, G.C., Araya, C., Green, M.J., Clark, B.S., Link, B.A., and Clarke, J.D. (2013) Mirror-symmetric microtubule assembly and cell interactions drive lumen formation in the zebrafish neural rod. The EMBO journal. 32(1):30-44
Hayes, J.M., Hartsock, A., Clark, B.S., Napier, H.R., Link, B.A., and Gross, J.M. (2012) Integrin α5/fibronectin1 and focal adhesion kinase are required for lens fiber morphogenesis in zebrafish. Molecular biology of the cell. 23(24):4725-4738
Clark, B.S., Cui, S., Miesfeld, J.B., Klezovitch, O., Vasioukhin, V., and Link, B.A. (2012) Loss of Llgl1 in retinal neuroepithelia reveals links between apical domain size, Notch activity and neurogenesis. Development (Cambridge, England). 139(9):1599-1610
Gestri, G., Link, B.A., and Neuhauss, S.C. (2012) The visual system of zebrafish and its use to model human ocular diseases. Developmental Neurobiology. 72(3):302-327
Clark, B.S., Winter, M., Cohen, A.R., and Link, B.A. (2011) Generation of Rab-based transgenic lines for in vivo studies of endosome biology in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(11):2452-2465
Bachmann-Gagescu, R., Phelps, I.G., Stearns, G., Link, B.A., Brockerhoff, S.E., Moens, C.B., and Doherty, D. (2011) The Ciliopathy Gene cc2d2a controls Zebrafish Photoreceptor Outer Segment Development Through a Role in Rab8-dependent Vesicle Trafficking. Human molecular genetics. 20(20):4041-55
Kur, E., Christa, A., Veth, K.N., Gajera, C.R., Andrade-Navarro, M.A., Zhang, J., Willer, J.R., Gregg, R.G., Abdelilah-Seyfried, S., Bachmann, S., Link, B.A., Hammes, A., and Willnow, T.E. (2011) Loss of Lrp2 in zebrafish disrupts pronephric tubular clearance but not forebrain development. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(6):1567-1577
Volkmann, B.A., Zinkevich, N.S., Mustonen, A., Schilter, K.F., Bosenko, D.V., Reis, L.M., Broeckel, U., Link, B.A., and Semina, E. (2011) Potential novel mechanism for Axenfeld-Rieger syndrome: deletion of a distant region containing regulatory elements of PITX2. Investigative ophthalmology & visual science. 52(3):1450-1459
Collery, R.F., and Link, B.A. (2011) Dynamic smad-mediated BMP signaling revealed through transgenic zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 240(3):712-722
Veth, K.N., Willer, J.R., Collery, R.F., Gray, M.P., Willer, G.B., Wagner, D.S., Mullins, M.C., Udvadia, A.J., Smith, R.S., John, S.W., Gregg, R.G., and Link, B.A. (2011) Mutations in Zebrafish lrp2 Result in Adult-Onset Ocular Pathogenesis That Models Myopia and Other Risk Factors for Glaucoma. PLoS Genetics. 7(2):e1001310
Choi, J.H., Law, M.Y., Chien, C.B., Link, B.A., and Wong, R.O. (2010) In vivo development of dendritic orientation in wild-type and mislocalized retinal ganglion cells. Neural Development. 5:29
Insinna, C., Baye, L.M., Amsterdam, A., Besharse, J.C., and Link, B.A. (2010) Analysis of a zebrafish dync1h1 mutant reveals multiple functions for cytoplasmic dynein 1 during retinal photoreceptor development. Neural Development. 5:12
Ponnuswami, A., Cliff, M., and Link, B.A. (2010) Transgenic Line Submission. ZFIN Direct Data Submission.
Skarie, J.M., and Link, B.A. (2009) FoxC1 is essential for vascular basement membrane integrity and hyaloid vessel morphogenesis. Investigative ophthalmology & visual science. 50(11):5026-5034
Norden, C., Young, S., Link, B.A., and Harris, W.A. (2009) Actomyosin is the main driver of interkinetic nuclear migration in the retina. Cell. 138(6):1195-1208
McMahon, C., Gestri, G., Wilson, S.W., and Link, B.A. (2009) Lmx1b is essential for survival of periocular mesenchymal cells and influences Fgf-mediated retinal patterning in zebrafish. Developmental Biology. 332(2):287-298
Gray, M.P., Smith, R.S., Soules, K.A., John, S.W., and Link, B. (2009) The aqueous humor outflow pathway of zebrafish. Investigative ophthalmology & visual science. 50(4):1515-1521
Hava, D., Forster, U., Matsuda, M., Cui, S., Link, B.A., Eichhorst, J., Wiesner, B., Chitnis, A., and Abdelilah-Seyfried, S. (2009) Apical membrane maturation and cellular rosette formation during morphogenesis of the zebrafish lateral line. Journal of Cell Science. 122(Pt 5):687-695
Del Bene, F., Wehman, A.M., Link, B.A., and Baier, H. (2008) Regulation of neurogenesis by interkinetic nuclear migration through an apical-basal notch gradient. Cell. 134(6):1055-1065
Leung, Y.F., Ma, P., Link, B.A., and Dowling, J.E. (2008) Factorial microarray analysis of zebrafish retinal development. Proceedings of the National Academy of Sciences of the United States of America. 105(35):12909-12914
Skarie, J.M., and Link, B.A. (2008) The Primary open-angle glaucoma gene WDR36 functions in ribosomal RNA processing and interacts with the p53 stress–response pathway. Human molecular genetics. 17(16):2474-2485
Berry, F.B., Skarie, J.M., Mirzayans, F., Fortin, Y., Hudson, T.J., Raymond, V., Link, B.A., and Walter, M.A. (2008) FOXC1 is required for cell viability and resistance to oxidative stress in the eye through the transcriptional regulation of FOXO1A. Human molecular genetics. 17(4):490-505
Baye, L.M., and Link, B.A. (2007) Interkinetic nuclear migration and the selection of neurogenic cell divisions during vertebrate retinogenesis. The Journal of neuroscience : the official journal of the Society for Neuroscience. 27(38):10143-10152
Baye, L.M., and Link, B.A. (2007) The disarrayed mutation results in cell cycle and neurogenesis defects during retinal development in zebrafish. BMC Developmental Biology. 7(1):28
Cui, S., Otten, C., Rohr, S., Abdelilah-Seyfried, S., and Link, B.A. (2007) Analysis of aPKClambda and aPKCzeta reveals multiple and redundant functions during vertebrate retinogenesis. Molecular and cellular neurosciences. 34(3):431-444
Semina, E.V., Bosenko, D.V., Zinkevich, N.C., Soules, K.A., Hyde, D.R., Vihtelic, T.S., Willer, G.B., Gregg, R.G., and Link, B.A. (2006) Mutations in laminin alpha 1 result in complex, lens-independent ocular phenotypes in zebrafish. Developmental Biology. 299(1):63-77
Tamimi, Y., Skarie, J.M., Footz, T., Berry, F.B., Link, B.A., and Walter, M.A. (2006) FGF19 is a target for FOXC1 regulation in ciliary body derived cells. Human molecular genetics. 15(21):3229-3240
Zinkevich, N.S., Bosenko, D.V., Link, B.A., and Semina, E.V. (2006) laminin alpha 1 gene is essential for normal lens development in zebrafish. BMC Developmental Biology. 6:13
Willer, G.B., Lee, V.M., Gregg, R.G., and Link, B.A. (2005) Analysis of the zebrafish perplexed mutation reveals tissue specific roles for de novo pyrimidine synthesis during development. Genetics. 170(4):1827-1837
Soules, K.A., and Link, B.A. (2005) Morphogenesis of the anterior segment in the zebrafish eye. BMC Developmental Biology. 5:12
Kay, J.N., Link, B.A., and Baier, H. (2005) Staggered cell-intrinsic timing of ath5 expression underlies the wave of ganglion cell neurogenesis in the zebrafish retina. Development (Cambridge, England). 132(11):2573-2585
Perkins, B.D., Nicholas, C.S., Baye, L.M., Link, B.A., and Dowling, J.E. (2005) dazed gene is necessary for late cell type development and retinal cell maintenance in the zebrafish retina. Developmental Dynamics : an official publication of the American Association of Anatomists. 233(2):680-694
Link, B.A., Gray, M.P., Smith, R.S., and John, S.W. (2004) Intraocular Pressure in Zebrafish: Comparison of Inbred Strains and Identification of a Reduced Melanin Mutant with Raised IOP. Investigative ophthalmology & visual science. 45(12):4415-4422
McMahon, C., Semina, E.V., and Link, B.A. (2004) Using zebrafish to study the complex genetics of glaucoma. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 138(3):343-350
Gregg, R.G., Willer, G.B., Fadool, J.M., Dowling, J.E., and Link, B.A. (2003) Positional cloning of the young mutation identifies an essential role for the Brahma chromatin remodeling complex in mediating retinal cell differentiation. Proceedings of the National Academy of Sciences of the United States of America. 100(11):6535-6540
Kurita, R., Sagara, H., Aoki, Y., Link, B.A., Arai, K.-I., and Watanabe, S. (2003) Suppression of lens growth by alphaA-crystallin promoter-driven expression of diphtheria toxin results in disruption of retinal cell organization in zebrafish. Developmental Biology. 255(1):113-127
Link, B.A. (2001) Evidence for directed mitotic cleavage plane reorientations during retinal development within the zebrafish. The Biological bulletin. 201(2):254-255
Link, B.A., Kainz, P.M., Ryou, T., and Dowling, J.E. (2001) The perplexed and confused mutations affect distinct stages during the transition from proliferating to post-mitotic cells within the zebrafish retina. Developmental Biology. 236(2):436-453
Link, B.A., Darland, T., and Dowling, J.E. (2001) Isolation of zebrafish mutations that affect the development and maintenance of the lens. Investigative ophthalmology & visual science. 42(4):2882
Link, B.A. and Darland, T. (2001) Genetic analysis of initial and ongoing retinogenesis in the zebrafish: comparing the central neuroepithelium and marginal zone. Progress in brain research. 131:565-577
Peterson, R.T., Link, B.A., Dowling, J.E., and Schreiber, S.L. (2000) Small molecule developmental screens reveal the logic and timing of vertebrate development. Proceedings of the National Academy of Sciences of the United States of America. 97(24):12965-12969
Link, B.A., Fadool, J.M., Malicki, J., and Dowling, J.E. (2000) The zebrafish young mutation acts non-cell-autonomously to uncouple differentiation from specification for all retinal cells. Development (Cambridge, England). 127(10):2177-2188