ZFIN ID: ZDB-PUB-090106-17 |
Distinct functions of Wnt/{beta}-catenin signaling in KV development and cardiac asymmetry
Lin, X., and Xu, X.
Date: | 2009 |
---|---|
Source: | Development (Cambridge, England) 136(2): 207-217 (Journal) |
Registered Authors: | Lin, Xueying, Xu, Xiaolei |
Keywords: | Left-right asymmetry, Wnt signaling, Gata4, Lefty2, Zebrafish |
MeSH Terms: |
|
PubMed: | 19103803 Full text @ Development |
Citation
Lin, X., and Xu, X. (2009) Distinct functions of Wnt/{beta}-catenin signaling in KV development and cardiac asymmetry. Development (Cambridge, England). 136(2):207-217.
ABSTRACT
The Wnt/beta-catenin pathway exhibits distinct and developmental stage-specific roles during cardiogenesis. However, little is known about the molecular mechanisms of Wnt/beta-catenin signaling in the establishment of cardiac left-right (LR) asymmetry. Using zebrafish as an animal model, we show here that Wnt/beta-catenin signaling is differentially required in cardiac LR patterning. At an early stage, during asymmetric signal generation, Wnt/beta-catenin signaling is necessary for Kupffer's vesicle development and for the regulation of both heart and visceral laterality. At a later stage, during asymmetric signal propagation, excessive Wnt/beta-catenin signaling inhibits the transmission of asymmetric cues from the lateral plate mesoderm (LPM) to the cardiac field but not to the developing gut; as such, it only regulates heart laterality. Molecular analysis identifies Gata4 as the downstream target of Wnt/beta-catenin signaling in the cardiac field that responds to the Wnt/beta-catenin signaling and regulates the competence of the heart field to express left-sided genes. In summary, our results reveal a previously unexpected role of Wnt-Gata4 signaling in the control of asymmetric signal propagation from the LPM to the cardiac field.
ADDITIONAL INFORMATION
- Genes / Markers (18)
- Morpholino (6)
- Engineered Foreign Genes (1)
- Expression and Phenotype Data
- Mutations and Transgenics (2)
- Fish (11)