PUBLICATION
Distinct functions of Wnt/{beta}-catenin signaling in KV development and cardiac asymmetry
- Authors
- Lin, X., and Xu, X.
- ID
- ZDB-PUB-090106-17
- Date
- 2009
- Source
- Development (Cambridge, England) 136(2): 207-217 (Journal)
- Registered Authors
- Lin, Xueying, Xu, Xiaolei
- Keywords
- Left-right asymmetry, Wnt signaling, Gata4, Lefty2, Zebrafish
- MeSH Terms
-
- Genes, APC
- Left-Right Determination Factors/genetics
- Left-Right Determination Factors/physiology
- Base Sequence
- Animals
- Animals, Genetically Modified
- beta Catenin/genetics
- beta Catenin/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Wnt Proteins/genetics
- Wnt Proteins/physiology*
- GATA Transcription Factors/genetics
- GATA Transcription Factors/physiology
- Heart/embryology*
- Wnt3 Protein
- Models, Cardiovascular
- Gene Expression Regulation, Developmental
- Signal Transduction
- DNA Primers/genetics
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/physiology*
- Body Patterning/genetics
- Body Patterning/physiology
- PubMed
- 19103803 Full text @ Development
Citation
Lin, X., and Xu, X. (2009) Distinct functions of Wnt/{beta}-catenin signaling in KV development and cardiac asymmetry. Development (Cambridge, England). 136(2):207-217.
Abstract
The Wnt/beta-catenin pathway exhibits distinct and developmental stage-specific roles during cardiogenesis. However, little is known about the molecular mechanisms of Wnt/beta-catenin signaling in the establishment of cardiac left-right (LR) asymmetry. Using zebrafish as an animal model, we show here that Wnt/beta-catenin signaling is differentially required in cardiac LR patterning. At an early stage, during asymmetric signal generation, Wnt/beta-catenin signaling is necessary for Kupffer's vesicle development and for the regulation of both heart and visceral laterality. At a later stage, during asymmetric signal propagation, excessive Wnt/beta-catenin signaling inhibits the transmission of asymmetric cues from the lateral plate mesoderm (LPM) to the cardiac field but not to the developing gut; as such, it only regulates heart laterality. Molecular analysis identifies Gata4 as the downstream target of Wnt/beta-catenin signaling in the cardiac field that responds to the Wnt/beta-catenin signaling and regulates the competence of the heart field to express left-sided genes. In summary, our results reveal a previously unexpected role of Wnt-Gata4 signaling in the control of asymmetric signal propagation from the LPM to the cardiac field.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping