PUBLICATION
Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP
- Authors
- Liu, J., Sun, Y.H., Wang, Y.W., Wang, N., Pei, D.S., Wang, Y.P., Hu, W., and Zhu, Z.Y.
- ID
- ZDB-PUB-080826-5
- Date
- 2008
- Source
- Theriogenology 70(9): 1525-1535 (Journal)
- Registered Authors
- Hu, Wei, Pei, Desheng, Sun, Yonghua, Zhu, Zuoyan
- Keywords
- Zebrafish, Chinese rare minnow, Crossbred embryo, cDNA-AFLP, Differential expression
- MeSH Terms
-
- Amplified Fragment Length Polymorphism Analysis/veterinary*
- Animals
- Crosses, Genetic
- Cyprinidae/embryology*
- Cyprinidae/genetics
- DNA, Complementary/genetics
- Fish Proteins/genetics
- Fish Proteins/metabolism*
- Gene Expression Profiling*
- Gene Expression Regulation, Developmental/physiology
- Gene Silencing
- Transcription, Genetic
- Zebrafish/embryology*
- Zebrafish/genetics
- PubMed
- 18692889 Full text @ Theriogenology
Citation
Liu, J., Sun, Y.H., Wang, Y.W., Wang, N., Pei, D.S., Wang, Y.P., Hu, W., and Zhu, Z.Y. (2008) Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP. Theriogenology. 70(9):1525-1535.
Abstract
The crosstalk between naive nucleus and maternal factors deposited in egg cytoplasm before zygotic genome activation is crucial for early development. In this study, we utilized two laboratory fishes, zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus), to obtain mutual crossbred embryos and examine the interaction between nucleus and egg cytoplasm from different species. Although these two types of crossbred embryos originated from common nuclei, various developmental capacities were gained due to different origins of the egg cytoplasm. Using cDNA amplified fragment length polymorphism (cDNA-AFLP), we compared transcript profiles between the mutual crossbred embryos at two developmental stages (50%- and 90%-epiboly). Three thousand cDNA fragments were generated in four cDNA pools with 64 primer combinations. All differentially displayed transcript-derived fragments (TDFs) were screened by dot blot hybridization, and the selected sequences were further analyzed by semi-quantitative RT-PCR and quantitative real-time RT-PCR. Compared with ZR embryos, 12 genes were up-regulated and 12 were down-regulated in RZ embryos. The gene fragments were sequenced and subjected to BLASTN analysis. The sequences encoded various proteins which functioned at various levels of proliferation, growth, and development. One gene (ZR6), dramatically down-regulated in RZ embryos, was chosen for loss-of-function study; the knockdown of ZR6 gave rise to the phenotype resembling that of RZ embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping