PUBLICATION

CREB activity modulates neural cell proliferation, midbrain-hindbrain organization and patterning in zebrafish

Authors
Dworkin, S., Heath, J.K., Dejong-Curtain, T.A., Hogan, B.M., Lieschke, G.J., Malaterre, J., Ramsay, R.G., and Mantamadiotis, T.
ID
ZDB-PUB-070614-18
Date
2007
Source
Developmental Biology   307(1): 127-141 (Journal)
Registered Authors
de Jong-Curtain, Tanya A., Heath, Joan K., Hogan, Ben M., Lieschke, Graham J.
Keywords
CREB, Neurogenesis, Midbrain–hindbrain boundary, Zebrafish
MeSH Terms
  • Animals
  • Body Patterning*
  • Cell Proliferation*
  • Central Nervous System/growth & development
  • Cyclic AMP Response Element-Binding Protein/physiology*
  • Mesencephalon/physiology*
  • Neurons/cytology*
  • Phosphorylation
  • Rhombencephalon/physiology*
  • Zebrafish
PubMed
17531969 Full text @ Dev. Biol.
Abstract
Neural stem/progenitor cells (NPCs) self-renew and differentiate, generating neuronal and non-neuronal (glial) cell lineages. Although a number of factors, including transcription factors, have been shown to be important in the regulation of NPC proliferation and differentiation, the precise molecular networks remain to be identified. The cAMP Response Element-Binding protein (CREB) is a transcription factor important for neuronal survival, differentiation and plasticity. Recent work suggests that CREB activation, via serine phosphorylation in the kinase inducible domain, is important for neurogenesis in the adult rodent brain. We sought to further investigate CREB function in neurogenesis, using the zebrafish (Danio rerio). Structural and functional analysis of the zebrafish CREB orthologue showed high conservation with mammalian CREB. Activated (phosphorylated) CREB (pCREB) was localised to all known proliferation zones in the adult zebrafish brain, including actively cycling cells. Furthermore, we found that modulating CREB activity during early zebrafish development caused significant defects in neural proliferation, midbrain-hindbrain organization and body patterning. These findings reveal broader and stage-specific physiological roles of CREB function during vertebrate neural development and proliferation.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes