PUBLICATION

The G protein-coupled receptor agtrl1b regulates early development of myocardial progenitors

Authors
Scott, I.C., Masri, B., D'Amico, L.A., Jin, S.W., Jungblut, B., Wehman, A.M., Baier, H., Audigier, Y., and Stainier, D.Y.
ID
ZDB-PUB-070310-9
Date
2007
Source
Developmental Cell   12(3): 403-413 (Journal)
Registered Authors
Baier, Herwig, D'Amico, Leonard, Jin, Suk-Won, Jungblut, Benno, Scott, Ian, Stainier, Didier, Wehman, Ann
Keywords
none
MeSH Terms
  • Animals
  • Animals, Genetically Modified/embryology
  • Animals, Genetically Modified/genetics
  • Cell Differentiation/genetics
  • Cell Movement/genetics
  • Gastrula/cytology
  • Gastrula/metabolism
  • Gene Expression Regulation, Developmental/genetics
  • Heart/embryology*
  • Intercellular Signaling Peptides and Proteins/genetics
  • Intercellular Signaling Peptides and Proteins/metabolism*
  • Mesoderm/cytology
  • Mesoderm/metabolism
  • Mutation/genetics
  • Myoblasts, Cardiac/cytology
  • Myoblasts, Cardiac/metabolism*
  • Myocardium/cytology
  • Myocardium/metabolism
  • Organogenesis/genetics*
  • Receptors, G-Protein-Coupled/genetics
  • Receptors, G-Protein-Coupled/metabolism*
  • Receptors, G-Protein-Coupled/physiology
  • Xenopus Proteins/genetics
  • Xenopus Proteins/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed
17336906 Full text @ Dev. Cell
Abstract
While many factors that modulate the morphogenesis and patterning of the embryonic heart have been identified, relatively little is known about the molecular events that regulate the differentiation of progenitor cells fated to form the myocardium. Here, we show that zebrafish grinch (grn) mutants form a reduced number of myocardial progenitor cells, which results in a profound deficit in cardiomyocyte numbers in the most severe cases. We show that grn encodes the G protein-coupled receptor (GPCR) Agtrl1b, a known regulator of adult cardiovascular physiology. Ectopic expression of Apelin, an Agtrl1b ligand, results in the complete absence of cardiomyocytes. Data from transplantation and transgenic approaches indicate that Agtrl1 signaling plays a cell-autonomous role in myocardial specification, with activity being required coincident with the onset of gastrulation movements. These results support a model in which agtrl1b regulates the migration of cells fated to form myocardial progenitors.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes