PUBLICATION

hecate, a zebrafish maternal effect gene, affects dorsal organizer induction and intracellular calcium transient frequency

Authors

Lyman Gingerich, J., Westfall, T.A., Slusarski, D.C., and Pelegri, F.

ID

ZDB-PUB-050913-5

Date

2005

Source

Developmental Biology 286(2): 427-439 (Journal)

Registered Authors

Pelegri, Francisco, Slusarski, Diane C.

Keywords

Wnt signaling, B-catenin, Calcium, hecate, Axis induction, Maternal effect, Zebrafish

MeSH Terms

Gene Expression Regulation, Developmental
Wnt Proteins/metabolism
Zebrafish/embryology*
Mutation*
beta Catenin/metabolism
Choristoma/genetics*
Calcium/metabolism*
Animals
Organizers, Embryonic/embryology*

(all 9)

PubMed

16154557 Full text @ Dev. Biol.

Abstract

A zebrafish maternal effect mutation, in the gene hecate, results in embryos that have defects in the formation of dorsoanterior structures and altered calcium release. hecate mutant embryos lack nuclear accumulation of β-catenin and have reduced expression of genes specific to the dorsal organizer. We found that hecate mutant embryos exhibit a nearly 10-fold increase in the frequency of intracellular Ca(2+) transients normally present in the enveloping layer during the blastula stages. Inhibition of Ca(2+) release leads to ectopic expression of dorsal genes in mutant embryos suggesting that Ca(2+) transients are important in mediating dorsal gene expression. Inhibition of Ca(2+) release also results in the expression of dorsal-specific genes in the enveloping layer in a β-catenin-independent manner, which suggests an additional function for the Ca(2+) transients in this cellular layer. The mutant phenotype can be reversed by the expression of factors that activate Wnt/β-catenin signaling, suggesting that the Wnt/β-catenin pathway, at least as activated by an exogenous Wnt ligand, is intact in hec mutant embryos. Our results are consistent with a role for the hecate gene in the regulation of Ca(2+) release during the cleavage stages, which in turn influences dorsal gene expression in both marginal cells along the dorsoventral axis and in the enveloping layer.

Genes / Markers

Figures

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Expression

Fish	Conditions	Stage	Anatomy	Assay	Figure
AB	standard conditions	75%-epiboly	unspecified	ISH	Fig. 2
grip2a^t2800	standard conditions	75%-epiboly	unspecified	ISH	Fig. 2
AB	chemical treatment: thapsigargin	30%-epiboly	DEL	ISH	Fig. 5
AB	control	30%-epiboly	DEL	ISH	Fig. 5
AB	control	30%-epiboly	EVL	ISH	Fig. 5
AB	chemical treatment: thapsigargin	30%-epiboly	EVL	ISH	Fig. 5
AB	control	50%-epiboly	margin	ISH	Fig. 5
AB	chemical treatment: thapsigargin	50%-epiboly	margin	ISH	Fig. 5
AB	control	Sphere	marginal blastomere	ISH	Fig. 5
AB	chemical treatment: thapsigargin	Sphere	marginal blastomere	ISH	Fig. 5

1 - 10 of 28

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
grip2a^t2800	standard conditions	Shield	dorsal/ventral pattern formation disrupted, abnormal	Fig. 1
grip2a^t2800	standard conditions	Shield	shield aplastic, abnormal	Fig. 1
grip2a^t2800	standard conditions	Shield	whole organism wholly ventralized, abnormal	Fig. 1
grip2a^t2800	standard conditions	Prim-5	axis specification disrupted, abnormal	Fig. 1
grip2a^t2800	standard conditions	Prim-5	eye decreased size, abnormal	Fig. 1
grip2a^t2800	standard conditions	Prim-5	forebrain dorsal region aplastic, abnormal	Fig. 1
grip2a^t2800	standard conditions	Prim-5	head cell undifferentiated, abnormal	Fig. 1
grip2a^t2800	standard conditions	Prim-5	head decreased size, abnormal	Fig. 1
grip2a^t2800	standard conditions	Prim-5	notochord aplastic, abnormal	Fig. 1
grip2a^t2800	standard conditions	Prim-5	somite cuboid, abnormal	Fig. 1

1 - 10 of 11

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
t2800		Point Mutation	grip2a

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
ctnnb1	MO1-ctnnb1	MRPHLNO

Fish

Fish
AB
AB + MO1-ctnnb1
grip2a^t2800
grip2a^t2800

Antibodies

Name	Type	Antigen Genes	Isotypes	Host Organism
Ab1-ctnnb	monoclonal		IgG1	Mouse

Orthology

Engineered Foreign Genes

Mapping

Lyman Gingerich et al., 2005 ZDB-PUB-050913-5 PMID:16154557

hecate, a zebrafish maternal effect gene, affects dorsal organizer induction and intracellular calcium transient frequency

Lyman Gingerich et al., 2005

ZDB-PUB-050913-5
PMID:16154557