ZFIN ID: ZDB-PERS-960805-452
Raymond, Pamela
Email: praymond@umich.edu
URL: https://sites.lsa.umich.edu/raymond-lab/
Affiliation: Raymond Lab
Address: Department of Molecular, Cellular, and Developmental Biology University of Michigan 5238 Biological Sciences Building Ann Arbor, MI 48109-10
Country: United States
Phone: 734-647-0811
Fax: 734-763-1166
ORCID ID: 0000-0003-1083-5530

Professor Raymond retired in June, 2017.

The research in Pamela Raymond's laboratory was concerned with retinal neurogenesis, neural stem cells, and regeneration. The overall objective was to understand the molecular basis of cell-cell interactions that regulate retinal neurogenesis (the formation of neurons) and neuronal specificity (the expression of differentiated cellular features) during development and regeneration. We used zebrafish as a model system, and we concentrated on a selected subset of molecular interactions that are likely to be involved in determination of cell fate in retinal stem cells and progenitor cells: cell surface signaling molecules - such as the cadherin family of adhesion molecules, the Notch-Delta signaling pathway, and other signaling systems, and transcriptional regulators - especially the family of paired-like homeodomain proteins that have been implicated in the regionalization of brain territories, the control of proliferation, and the determination of specified progenitor cells and differentiated cell fates. We were also interested in understanding the cellular and molecular mechanisms of neuronal pattern formation, especially the formation of precise arrays of cone photoreceptors in the teleost fish retina.

Nunley, H., Nagashima, M., Martin, K., Lorenzo Gonzalez, A., Suzuki, S.C., Norton, D.A., Wong, R.O.L., Raymond, P.A., Lubensky, D.K. (2020) Defect patterns on the curved surface of fish retinae suggest a mechanism of cone mosaic formation. PLoS Computational Biology. 16:e1008437
Nagashima, M., D'Cruz, T.S., Danku, A.E., Hesse, D., Sifuentes, C., Raymond, P.A., Hitchcock, P.F. (2019) Midkine-a is required for cell cycle progression of Müller glia glia during neuronal regeneration in the vertebrate retina. The Journal of neuroscience : the official journal of the Society for Neuroscience. 40(6):1232-1247
Fu, J., Nagashima, M., Guo, C., Raymond, P.A., Wei, X. (2018) Novel Animal Model of Crumbs-Dependent Progressive Retinal Degeneration That Targets Specific Cone Subtypes. Investigative ophthalmology & visual science. 59:505-518
Nagashima, M., Hadidjojo, J., Barthel, L.K., Lubensky, D.K., Raymond, P.A. (2017) Anisotropic Müller glial scaffolding supports a multiplex lattice mosaic of photoreceptors in zebrafish retina. Neural Development. 12:20
Sifuentes, C.J., Kim, J.W., Swaroop, A., Raymond, P.A. (2016) Rapid, Dynamic Activation of Müller Glial Stem Cell Responses in Zebrafish. Investigative ophthalmology & visual science. 57:5148-5160
Raymond, P.A. (2016) Regeneration: New Neurons Wire Up. Current biology : CB. 26:R794-R796
Yoshimatsu, T., Williams, P.R., D'Orazi, F.D., Suzuki, S.C., Fadool, J.M., Allison, W.T., Raymond, P.A., Wong, R.O. (2014) Transmission from the dominant input shapes the stereotypic ratio of photoreceptor inputs onto horizontal cells. Nature communications. 5:3699
Lenkowski, J.R., and Raymond, P.A. (2014) Müller glia: Stem cells for generation and regeneration of retinal neurons in teleost fish. Progress in Retinal and Eye Research. 40C:94-123
Raymond, P.A., Colvin, S.M., Jabeen, Z., Nagashima, M., Barthel, L.K., Hadidjojo, J., Popova, L., Pejaver, V.R., and Lubensky, D.K. (2014) Patterning the cone mosaic array in zebrafish retina requires specification of ultraviolet-sensitive cones. PLoS One. 9(1):e85325
Nagashima, M., Barthel, L.K., and Raymond, P.A. (2013) A self-renewing division of zebrafish Muller glial cells generates neuronal progenitors that require N-cadherin to regenerate retinal neurons. Development (Cambridge, England). 140(22):4510-4521
Lenkowski, J.R., Qin, Z., Sifuentes, C.J., Thummel, R., Soto, C.M., Moens, C.B., Raymond, P.A.. (2013) Retinal regeneration in adult zebrafish requires regulation of TGFβ signaling. Glia. 61(10):1687-1697
Meyers, J.R., Hu, L., Moses, A., Kaboli, K., Papandrea, A., and Raymond, P.A. (2012) beta-catenin/Wnt signaling controls progenitor fate in the developing and regenerating zebrafish retina. Neural Development. 7(1):30
Salbreux, G., Barthel, L.K., Raymond, P.A., and Lubensky, D.K. (2012) Coupling mechanical deformations and planar cell polarity to create regular patterns in the zebrafish retina. PLoS Computational Biology. 8(8):e1002618
Qin, Z., and Raymond, P.A. (2012) Microarray-based gene profiling analysis of müller glia-derived retinal stem cells in light-damaged retinas from adult zebrafish. Methods in molecular biology (Clifton, N.J.). 884:255-261
Qin, Z., Kidd, A.R., Thomas, J.L., Poss, K.D., Hyde, D.R., Raymond, P.A., and Thummel, R. (2011) FGF signaling regulates rod photoreceptor cell maintenance and regeneration in zebrafish. Experimental Eye Research. 93(5):726-34
Allison, W.T., Barthel, L.K., Skebo, K.M., Takechi, M., Kawamura, S., and Raymond, P.A. (2010) Ontogeny of cone photoreceptor mosaics in zebrafish. The Journal of comparative neurology. 518(20):4182-4195
Calinescu, A.A., Raymond, P.A., and Hitchcock, P.F. (2009) Midkine expression is regulated by the circadian clock in the retina of the zebrafish. Visual neuroscience. 26(5-6):495-501
Qin, Z., Barthel, L.K., and Raymond, P.A. (2009) Genetic evidence for shared mechanisms of epimorphic regeneration in zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 106(23):9310-9315
Buchner, D.A., Su, F., Yamaoka, J.S., Kamei, M., Shavit, J.A., Barthel, L.K., McGee, B., Amigo, J.D., Kim, S., Hanosh, A.W., Jagadeeswaran, P., Goldman, D., Lawson, N.D., Raymond, P.A., Weinstein, B.M., Ginsburg, D., and Lyons, S.E. (2007) pak2a mutations cause cerebral hemorrhage in redhead zebrafish. Proceedings of the National Academy of Sciences of the United States of America. 104(35):13996-140001
Bernardos, R.L., Barthel, L.K., Meyers, J.R., and Raymond, P.A. (2007) Late-stage neuronal progenitors in the retina are radial Müller glia that function as retinal stem cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 27(26):7028-7040
Catalano, A.E., Raymond, P.A., Goldman, D., and Wei, X. (2007) Zebrafish dou yan mutation causes patterning defects and extensive cell death in the retina. Developmental Dynamics : an official publication of the American Association of Anatomists. 236(5):1295-1306
Bernardos, R.L., and Raymond, P.A. (2006) GFAP transgenic zebrafish. Gene expression patterns : GEP. 6(8):1007-1013
Raymond, P.A., Barthel, L.K., Bernardos, R.L., and Perkowski, J.J. (2006) Molecular characterization of retinal stem cells and their niches in adult zebrafish. BMC Developmental Biology. 6:36
Wehman, A.M., Staub, W., Meyers, J.R., Raymond, P.A., and Baier, H. (2005) Genetic dissection of the zebrafish retinal stem-cell compartment. Developmental Biology. 281(1):53-65
Bernardos, R.L., Lentz, S.I., Wolfe, M.S., and Raymond, P.A. (2005) Notch-Delta signaling is required for spatial patterning and Muller glia differentiation in the zebrafish retina. Developmental Biology. 278(2):381-395
Hitchcock, P.F., and Raymond, P.A. (2004) The Teleost Retina as a Model for Developmental and Regeneration Biology. Zebrafish. 1(3):257-271
Shen, Y.C., and Raymond, P.A. (2004) Zebrafish cone-rod (crx) homeobox gene promotes retinogenesis. Developmental Biology. 269(1):237-251
Raymond, P.A., and Barthel, L.K. (2004) A moving wave patterns the cone photoreceptor mosaic array in the zebrafish retina. The International journal of developmental biology. 48(8-9):935-945
Novince, Z.M., Azodi, E., Marrs, J.A., Raymond, P.A., and Liu, Q. (2003) Cadherin expression in the inner ear of developing zebrafish. Gene expression patterns : GEP. 3(3):337-339
Liu, Q., Londraville, R.L., Azodi, E., Babb, S.G., Chiappini-Williamson, C., Marrs, J.A., and Raymond, P.A. (2002) Up-regulation of cadherin-2 and cadherin-4 in regenerating visual structures of adult zebrafish. Experimental neurology. 177(2):396-406
Chuang, J.-C. and Raymond, P.A. (2002) Embryonic origin of the eyes in teleost fish. BioEssays : news and reviews in molecular, cellular and developmental biology. 24(6):519-529
Gamse, J.T., Shen, Y.-C., Thisse, C., Thisse, B., Raymond, P.A., Halpern, M.E., and Liang, J.O. (2002) Otx5 regulates genes that show circadian expression in the zebrafish pineal complex. Nature Genetics. 30(1):117-121
Liu, Q., Marrs, J.A., Chuang, J.C., and Raymond, P.A. (2001) Cadherin-4 expression in the zebrafish central nervous system and regulation by ventral midline signaling. Brain research. Developmental brain research. 131(1-2):17-29
Liu, Q., Babb, S.G., Novince, Z.M., Doedens, A.L., Marrs, J., and Raymond, P.A. (2001) Differential expression of cadherin-2 and cadherin-4 in the developing and adult zebrafish visual system. Visual neuroscience. 18(6):923-933
Babb, S.G., Barnett, J., Doedens, A.L., Cobb, N., Liu, Q., Sorkin, B.C., Yelick, P.C., Raymond, P.A., and Marrs, J.A. (2001) Zebrafish E-cadherin: Expression during early embryogenesis and regulation during brain development. Developmental Dynamics : an official publication of the American Association of Anatomists. 221(2):231-237
Raymond, P.A. and Lentz, S.I. (2001) Regulation of retinal neurogenesis in zebrafish by notch signaling. Investigative ophthalmology & visual science. 42(4):1961
Chuang, J.-C. and Raymond, P.A. (2001) Zebrafish genes rx1 and rx2 help define the region of forebrain that gives rise to retina. Developmental Biology. 231:13-30
Liu, Y., Shen, Y.C., Rest, J.S., Raymond, P.A., and Zack, D.J. (2001) Isolation and characterization of a zebrafish homologue of the cone rod homeobox gene. Investigative ophthalmology & visual science. 42(2):481-487
Babb, S.G., Liu, Q., Raymond, P.A., Gallin, W.J., and Marrs, J.A. (2000) Zebrafish E-cadherin: phylogenic analysis, expression during early embryogenesis and regulation during midbrain-hindbrain boundary formation. Molecular biology of the cell. 11:2026
Stenkamp, D.L., Frey, R.A., Prabhudesai, S.N., and Raymond, P.A. (2000) Function for hedgehog genes in zebrafish retinal development. Developmental Biology. 220(2):238-252
Barthel, L.K. and Raymond, P.A. (2000) In situ hybridization studies of retinal neurons. In Vertebrate Phototransduction and the Visual Cycle, K. Palczewski, ed., Methods in Enzymology, vol.. :579-590
Chuang, J.C., Mathers, P.H., and Raymond, P.A. (1999) Expression of three Rx homeobox genes in embryonic and adult zebrafish. Mechanisms of Development. 84(1-2):195-198
Stenkamp D.L., Cunningham, L.L., Raymond, P.A., and Gonzalez-Fernandez, F. (1998) Novel expression pattern of interphotoreceptor retinoid-binding protein (IRBP) in the adult and developing zebrafish retina and RPE. Molecular Vision. 4:26
Passini, M.A., Raymond, P.A., Schechter, N. (1998) Vsx-2, a gene encoding a paired-type homeodomain, is expressed in the retina, hindbrain, and spinal cord during goldfish embryogenesis. Dev. Brain Res.. 109:129-135
Passini, M.A., Kurtzman, A.L., Canger, A.K., Asch, W.S., Wray, G.A., Raymond, P.A., and Schechter, N. (1998) Cloning of zebrafish vsx1: expression of a paired-like homeobox gene during CNS development. Developmental genetics. 23:128-141
Passini, M.A., Levine, E.M., Canger, A.K., Raymond, P.A., and Schechter, N. (1997) Vsx-1 and Vsx-2: differential expression of two paired-like homeobox genes during zebrafish and goldfish retinogenesis. The Journal of comparative neurology. 388(3):495-505
Rajendran, R.R., Van, Niel, E.E., Stenkamp, D.L., Cunningham, L.L., Raymond, P.A., and Gonzalez-Fernandez, F. (1996) Zebrafish interphotoreceptor retinoid-binding protein: differential circadian expression among cone subtypes. The Journal of experimental biology. 199(12):2775-2787
Raymond, P.A., Barthel, L.K., and Stenkamp, D.L. (1996) The zebrafish ultraviolet cone opsin reported previously is expressed in rods. Investigative ophthalmology & visual science. 37(5):948-950
Raymond, P.A., Barthel, L.K., and Curran, G.A. (1995) Developmental patterning of rod and cone photoreceptors in embryonic zebrafish. The Journal of comparative neurology. 359:537-550
Raymond, P.A., Barthel, L.K., Rounsifer, M.E., Sullivan, S.A., and Knight, J.K. (1993) Expression of rod and cone visual pigments in goldfish and zebrafish: a rhodopsin-like gene is expressed in cones. Neuron. 10:1161-1174