PUBLICATION

Pax8 and Pax2a function synergistically in otic specification, downstream of the Foxi1 and Dlx3b transcription factors

Authors

Hans, S., Liu, D., and Westerfield, M.

ID

ZDB-PUB-041006-7

Date

2004

Source

Development (Cambridge, England) 131(20): 5091-5102 (Journal)

Registered Authors

Hans, Stefan, Liu, Dong, Westerfield, Monte

Keywords

dlx3b, fgf3, fgf8, foxi1, Inner ear, Morpholino, Otic placode, pax8, pax2a, sox9a, sox9b, Zebrafish

MeSH Terms

Forkhead Transcription Factors
Zebrafish Proteins/metabolism*
DNA-Binding Proteins/metabolism*
Animals
Fibroblast Growth Factors/metabolism
Transcription Factors/metabolism*
High Mobility Group Proteins/metabolism
Paired Box Transcription Factors
PAX2 Transcription Factor
Ear/embryology*
Zebrafish/embryology*
Zebrafish/metabolism
Trans-Activators/metabolism*
Nuclear Proteins/metabolism*
Homeodomain Proteins/metabolism*
Fibroblast Growth Factor 3
SOX9 Transcription Factor
Fibroblast Growth Factor 8

(all 18)

PubMed

15459102 Full text @ Development

Abstract

The vertebrate inner ear arises from an ectodermal thickening, the otic placode, that forms adjacent to the presumptive hindbrain. Previous studies have suggested that competent ectodermal cells respond to Fgf signals from adjacent tissues and express two highly related paired box transcription factors Pax2a and Pax8 in the developing placode. We show that compromising the functions of both Pax2a and Pax8 together blocks zebrafish ear development, leaving only a few residual otic cells. This suggests that Pax2a and Pax8 are the main effectors downstream of Fgf signals. Our results further provide evidence that pax8 expression and pax2a expression are regulated by two independent factors, Foxi1 and Dlx3b, respectively. Combined loss of both factors eliminates all indications of otic specification. We suggest that the Foxi1-Pax8 pathway provides an early 'jumpstart' of otic specification that is maintained by the Dlx3b-Pax2a pathway.

Genes / Markers

Figures

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Expression

Gene	Fish	Conditions	Stage	Qualifier	Anatomy	Assay	Figure
cldna	foxi1^em1/em1	standard conditions	26+ somites		otic vesicle	ISH	Fig. 7
cldna	foxi1^em1/em1 + MO1-dlx3b	standard conditions	26+ somites	Not Detected	otic vesicle	ISH	Fig. 7
cldna	pax2a^tu29a/tu29a	standard conditions	26+ somites		otic vesicle	ISH	Fig. 2
cldna	WT	standard conditions	26+ somites		otic vesicle	ISH	Fig. 7
cldna	WT + MO1-dlx3b	standard conditions	26+ somites		otic vesicle	ISH	Fig. 7
dlx3b	foxi1^em1/em1	standard conditions	10-13 somites		otic placode	IHC	Fig. 6
dlx3b	foxi1^em1/em1	standard conditions	26+ somites		otic vesicle	IHC	Fig. 7
dlx3b	pax2a^tu29a/tu29a + MO4-pax8 + MO5-pax8	standard conditions	5-9 somites		ectoderm	ISH	Fig. 3
dlx3b	pax2a^tu29a/tu29a + MO4-pax8 + MO5-pax8	standard conditions	10-13 somites		otic placode	ISH	Fig. 3
dlx3b	WT	standard conditions	5-9 somites		ectoderm	ISH	Fig. 3

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Phenotype

Mutations / Transgenics

Allele	Type	Affected Genomic Region
em1	Indel	foxi1
ti282a	Point Mutation	fgf8a
tu29a	Point Mutation	pax2a

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
dlx3b	MO1-dlx3b	MRPHLNO
fgf3	MO2-fgf3	MRPHLNO
fgf3	MO3-fgf3	MRPHLNO
fgf3	MO1-fgf3	MRPHLNO
fgf8a	MO1-fgf8a	MRPHLNO
fgf8a	MO2-fgf8a	MRPHLNO
pax8	MO1-pax8	MRPHLNO
pax8	MO2-pax8	MRPHLNO
pax8	MO3-pax8	MRPHLNO
pax8	MO4-pax8	MRPHLNO

1 - 10 of 11

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Fish

Fish
fgf8a^{ti282a/ti282a}
fgf8a^{ti282a/ti282a} + MO4-pax8 + MO5-pax8
foxi1^em1/em1
foxi1^em1/em1 + MO1-dlx3b
pax2a^tu29a/tu29a
pax2a^tu29a/tu29a + MO4-pax8 + MO5-pax8
WT
WT + MO1-dlx3b
WT + MO1-fgf8a + MO2-fgf3 + MO2-fgf8a + MO3-fgf3

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Antibodies

Orthology

Engineered Foreign Genes

Mapping

Hans et al., 2004 ZDB-PUB-041006-7 PMID:15459102

Pax8 and Pax2a function synergistically in otic specification, downstream of the Foxi1 and Dlx3b transcription factors

Hans et al., 2004

ZDB-PUB-041006-7
PMID:15459102