PUBLICATION
Her5 acts as a prepattern factor that blocks neurogenin1 and coe2 expression upstream of Notch to inhibit neurogenesis at the midbrain-hindbrain boundary
- Authors
- Geling, A., Plessy, C., Rastegar, S., Strähle, U., and Bally-Cuif, L.
- ID
- ZDB-PUB-040402-3
- Date
- 2004
- Source
- Development (Cambridge, England) 131(9): 1993-2006 (Journal)
- Registered Authors
- Bally-Cuif, Laure, Geling, Andrea, Plessy, Charles, Rastegar, Sepand, Strähle, Uwe
- Keywords
- Her5, Hairy, Midbrain-hindbrain boundary, Zebrafish, Neurogenesis, Pre-patterning
- MeSH Terms
-
- Zebrafish Proteins/metabolism*
- Gene Expression Regulation, Developmental*
- DNA-Binding Proteins/genetics
- DNA-Binding Proteins/metabolism*
- Body Patterning*
- Animals
- Signal Transduction/physiology
- In Situ Hybridization
- Receptors, Notch
- Morphogenesis/physiology
- Rhombencephalon/cytology
- Rhombencephalon/metabolism*
- Transcription Factors/genetics
- Transcription Factors/metabolism*
- Basic Helix-Loop-Helix Transcription Factors
- Neurons/cytology
- Neurons/metabolism
- Zebrafish/anatomy & histology
- Zebrafish/embryology*
- Zebrafish/physiology
- Mesencephalon/cytology
- Mesencephalon/metabolism*
- Cell Differentiation/physiology
- Animals, Genetically Modified
- Enhancer Elements, Genetic
- Embryonic Structures/anatomy & histology
- Embryonic Structures/physiology
- Membrane Proteins/metabolism
- Transgenes
- PubMed
- 15056616 Full text @ Development
Citation
Geling, A., Plessy, C., Rastegar, S., Strähle, U., and Bally-Cuif, L. (2004) Her5 acts as a prepattern factor that blocks neurogenin1 and coe2 expression upstream of Notch to inhibit neurogenesis at the midbrain-hindbrain boundary. Development (Cambridge, England). 131(9):1993-2006.
Abstract
Neurogenesis in both vertebrates and invertebrates is tightly controlled in time and space involving both positive and negative regulators. We report here that the bHLH factor Her5 acts as a prepattern gene to prevent neurogenesis in the anlage of the midbrain/hindbrain boundary in the zebrafish neural plate. This involves selective suppression of both neurogenin1 (ngn1) and coe2 mRNA expression in a process that is independent of Notch signalling, and where inhibition of either ngn1 or coe2 expression is sufficient to prevent neuronal differentiation across the midbrain-hindbrain boundary. A ngn1 transgene faithfully responds to Her5 and deletion analysis of the transgene identifies an E-box in a ngn1 upstream enhancer to be required for repression by Her5. Together our data demonstrate a role of Her5 as a prepattern factor in the spatial definition of proneural domains in the zebrafish neural plate, in a manner similar to its Drosophila homologue Hairy.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping