ZFIN ID: ZDB-PUB-010315-10
Dopamine transporter expression distinguishes dopaminergic neurons from other catecholaminergic neurons in the developing zebrafish embryo
Holzschuh, J., Ryu, S., Aberger, F., and Driever, W.
Date: 2001
Source: Mechanisms of Development 101(1-2): 237-243 (Journal)
Registered Authors: Aberger, Fritz, Driever, Wolfgang, Holzschuh, Jochen, Ryu, Soojin
Keywords: zebrafish; dopaminergic system; catecholaminergic system; tyrosine hydroxylase; dopamine transporter; diencephalon; olfactory bulb; pretectum; retina; locus coeruleus; medulla; carotid body; Parkinson's disease
MeSH Terms: Amino Acid Sequence; Animals; Carrier Proteins/biosynthesis*; Catecholamines/metabolism*; Central Nervous System/embryology (all 28) expand
PubMed: 11231083 Full text @ Mech. Dev.
To characterize the formation of the dopaminergic system in the developing zebrafish CNS, we cloned cDNAs encoding tyrosine hydroxylase (th), an enzyme in dopamine synthesis, and the dopamine transporter (dat), a membrane transport protein which terminates dopamine action by re-uptake. Dopaminergic neurons are first detected between 18 and 19 h post-fertilization in a cluster of cells in the ventral diencephalon. Subsequently, th and dat detection identifies dopaminergic neurons in the olfactory bulb, the pretectum, the retina and the locus coeruleus. Neurons expressing th but not dat are adrenergic or noradrenergic, and are found in the locus coeruleus, the medulla, the likely analog of the carotid body, and precursors of the enteric and sympathetic nervous system.