ZFIN ID: ZDB-PERS-990722-4
Holzschuh, Jochen
Email: jochen.holzschuh@biologie.uni-freiburg.de
URL: http://www.biologie.uni-freiburg.de/data/bio1/holzschuh/
Affiliation: Holzschuh Lab
Address: Department of Developmental Biology Biology I University of Freiburg Hauptstraße 1 Freiburg, 79104 Germany
Country: Germany
Phone: Office:++49 (0)761-203 2516 Lab:++49 (0)761-203 2537
Fax: ++49 (0)761-203 2597

The vertebrate brain has to regulate manifold functions to assure the correct function and survival of an organism. For this bold venture diverse neuronal networks with various types of neurons are built during the development of this organism. One of these networks is the sensory system, which detects information about an organism’s internal and external environment and thereby determines, in part, the response of the organism. The sensory neurons of the peripheric nervous system have afferent connections to hindbrain neurons, which are controlling the function of these organs and in turn are regulated by other hindbrain neurons e.g. noradrenergic neurons (NA). These NA neurons process information from peripheral and central sensory systems and therefore involved in the control of behavioral states, cardiovascular functions, nociception, sympathetic function and coordinate respiratory activity.
Defects in brainstem NA neurons are implicated in several human respiratory disorders, like congenital central hypoventilation syndrome (CCHS), central sleep apnea (CSA) and sudden infant death syndrome (SIDS).

Our laboratory aims to understand how signaling molecules and neuron specific transcription factors cooperate to regulate the differentiation of NA neurons in the brainstem and regulate the development of the respiratory network in zebrafish. With our research we hope to gain a better understanding of respiratory disorders in humans.
Our lab also studies the differentiation of neural crest cells to neurons of the sympathetic ganglia and enteric ganglia of the peripheric nervous system.

Hu, Z., Holzschuh, J., Driever, W. (2015) Loss of DDB1 Leads to Transcriptional p53 Pathway Activation in Proliferating Cells, Cell Cycle Deregulation, and Apoptosis in Zebrafish Embryos. PLoS One. 10:e0134299
Reichenbach, B., Delalande, J.M., Kolmogorova, E., Prier, A., Nguyen, T., Smith, C.M., Holzschuh, J., and Shepherd, I.T. (2008) Endoderm-derived Sonic hedgehog and mesoderm Hand2 expression are required for enteric nervous system development in zebrafish. Developmental Biology. 318(1):52-64
Filippi, A., Duerr, K., Ryu, S., Willaredt, M., Holzschuh, J., and Driever, W. (2007) Expression and function of nr4a2, lmx1b, and pitx3 in zebrafish dopaminergic and noradrenergic neuronal development. BMC Developmental Biology. 7(1):135
Ryu, S., Mahler, J., Acampora, D., Holzschuh, J., Erhardt, S., Omodei, D., Simeone, A., and Driever, W. (2007) Orthopedia Homeodomain Protein Is Essential for Diencephalic Dopaminergic Neuron Development. Current biology : CB. 17(10):873-880
Isken, A., Holzschuh, J., Lampert, J.M., Fischer, L., Oberhauser, V., Palczewski, K., and von Lintig, J. (2007) Sequestration of retinyl esters is essential for retinoid signaling in the zebrafish embryo. The Journal of biological chemistry. 282(2):1144-1151
Ettl, A.K., Holzschuh, J., and Driever, W. (2006) The zebrafish mutation m865 affects formation of dopaminergic neurons and neuronal survival, and maps to a genetic interval containing the sepiapterin reductase locus. Anatomy and embryology. 211(7):73-86
Durr, K., Holzschuh, J., Filippi, A., Ettl, A.K., Ryu, S., Shepherd, I.T., and Driever, W. (2006) Differential Roles of Transcriptional Mediator Complex Subunits Crsp34/Med27, Crsp150/Med14 and Trap100/Med24 During Zebrafish Retinal Development. Genetics. 174(2):693-705
Ryu, S., Holzschuh, J., Mahler, J., and Driever, W. (2006) Genetic analysis of dopaminergic system development in zebrafish. Journal of neural transmission. Supplementum. 70:61-66
Ryu, S., Holzschuh, J., Erhardt, S., Ettl, A.K., and Driever, W. (2005) Depletion of minichromosome maintenance protein 5 in the zebrafish retina causes cell-cycle defect and apoptosis. Proceedings of the National Academy of Sciences of the United States of America. 102(51):18467-18472
Holzschuh, J., Wada, N., Wada, C., Schaffer, A., Javidan, Y., Tallafuss, A., Bally-Cuif, L., and Schilling, T.F. (2005) Requirements for endoderm and BMP signaling in sensory neurogenesis in zebrafish. Development (Cambridge, England). 132(16):3731-3742
Barrallo Gimeno, A., Holzschuh, J., Driever, W., and Knapik, E.W. (2004) Neural crest survival and differentiation in zebrafish depends on mont blanc/tfap2a gene function. Development (Cambridge, England). 131(7):1463-1477
Holzschuh, J., Barrallo Gimeno, A., Ettl, A.K., Durr, K., Knapik, E.W., and Driever, W. (2003) Noradrenergic neurons in the zebrafish hindbrain are induced by retinoic acid and require tfap2a for expression of the neurotransmitter phenotype. Development (Cambridge, England). 130(23):5741-5754
Holzschuh, J., Hauptmann, G., and Driever, W. (2003) Genetic analysis of the roles of Hh, FGF8, and nodal signaling during catecholaminergic system development in the zebrafish brain. The Journal of neuroscience : the official journal of the Society for Neuroscience. 23(13):5507-5519
Lampert, J.M., Holzschuh, J., Hessel, S., Driever, W., Vogt, K., and von Lintig, J. (2003) Provitamin A conversion to retinal via the beta,beta-carotene-15,15'-oxygenase (bcox) is essential for pattern formation and differentiation during zebrafish embryogenesis. Development (Cambridge, England). 130(10):2173-2186
Biemar, F., Baraldi, F., Devos, N., Holzschuh, J., Martial, J.A., Driever, W., and Peers, B. (2002) Analysis of meis genes expression in zebrafish suggests a role in the development of organs derived from the endoderm. Developmental Biology. 247(2):495
Holzschuh, J., Ryu, S., Aberger, F., and Driever, W. (2001) Dopamine transporter expression distinguishes dopaminergic neurons from other catecholaminergic neurons in the developing zebrafish embryo. Mechanisms of Development. 101(1-2):237-243

Grothe C, Meisinger C, Holzschuh J, Wewetzer K, Cattini P. Over-expression of the 18 kD and 21/23 kD fibroblast growth factor-2 isoforms in PC12 cells and Schwann cells results in altered cell morphology and growth.
Brain Res Mol Brain Res. 1998 Jun 1;57(1):97-105.

Holzschuh J
Erweiterte Cytotoxizitätstests als Ersatzmethode für den Fischtest
Tectum Verlag, Marburg. 1998 ISBN 3-8288-0325-3

Zahn T, Hauck C, Holzschuh J, Braunbeck T. Acute and sublethal toxicity of seepage waters from garbage dumps to permanent cell lines and primary cultures of hepatocytes from rainbow trout (Oncorhynchus mykiss): a novel approach to environmental risk assessment for chemicals and chemical mixtures.
Zentralbl Hyg Umweltmed. 1995 Jan;196(5):455-79.