PUBLICATION

A sphingosine-1-phosphate receptor regulates cell migration during vertebrate heart development

Authors
Kupperman, E., An, S., Osborne, N., Waldron, S., and Stainier, D.Y.
ID
ZDB-PUB-000824-19
Date
2000
Source
Nature   406(6792): 192-195 (Journal)
Registered Authors
Kupperman, Erik, Osborne, Nick, Stainier, Didier
Keywords
none
MeSH Terms
  • Alleles
  • Animals
  • Cell Movement
  • Cloning, Molecular
  • Embryonic and Fetal Development
  • GTP-Binding Proteins/metabolism
  • Heart/embryology*
  • Humans
  • Jurkat Cells
  • Lysophospholipids*
  • Molecular Sequence Data
  • Mutation
  • Myocardium/cytology
  • Myocardium/metabolism
  • Receptors, Cell Surface/physiology*
  • Receptors, G-Protein-Coupled*
  • Receptors, Lysophospholipid
  • Sphingosine/analogs & derivatives*
  • Sphingosine/metabolism
  • Transfection
  • Transplantation Chimera
  • Zebrafish
(all 22)
PubMed
10910360 Full text @ Nature
Abstract
Coordinated cell migration is essential in many fundamental biological processes including embryonic development, organogenesis, wound healing and the immune response. During organogenesis, groups of cells are directed to specific locations within the embryo. Here we show that the zebrafish miles apart (mil) mutation specifically affects the migration of the heart precursors to the midline. We found that mutant cells transplanted into a wild-type embryo migrate normally and that wild-type cells in a mutant embryo fail to migrate, suggesting that mil may be involved in generating an environment permissive for migration. We isolated mil by positional cloning and show that it encodes a member of the lysosphingolipid G-protein-coupled receptor family. We also show that sphingosine-1-phosphate is a ligand for Mil, and that it activates several downstream signalling events that are not activated by the mutant alleles. These data reveal a new role for lysosphingolipids in regulating cell migration during vertebrate development and provide the first molecular clues into the fusion of the bilateral heart primordia during organogenesis of the heart.
Genes / Markers
Marker Marker Type Name
dnmt1GENEDNA (cytosine-5-)-methyltransferase 1
foxa1GENEforkhead box A1
foxa2GENEforkhead box A2
foxa3GENEforkhead box A3
myl7GENEmyosin, light chain 7, regulatory
s1pr2GENEsphingosine-1-phosphate receptor 2
z3725SSLPZ3725
1 - 7 of 7
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Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
m93
    Point Mutation
    te273
      Point Mutation
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Fish
      1 - 2 of 2
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      Antibodies
      Orthology
      Engineered Foreign Genes
      No data available
      Mapping