PUBLICATION
Proviral insertions in the zebrafish hagoromo gene, encoding an F-box/WD40-repeat protein, cause stripe pattern anomalies
- Authors
- Kawakami, K., Amsterdam, A., Shimoda, N., Becker, T., Mugg, J., Shima, A., and Hopkins, N.
- ID
- ZDB-PUB-000510-5
- Date
- 2000
- Source
- Current biology : CB 10(8): 463-466 (Journal)
- Registered Authors
- Amsterdam, Adam, Becker, Thomas S., Hopkins, Nancy, Kawakami, Koichi, Mugg, Jennifer, Shima, Akihiro, Shimoda, Nobuyoshi
- Keywords
- none
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Body Patterning/genetics*
- DNA, Complementary/genetics
- F-Box Proteins
- Mice
- Molecular Sequence Data
- Mutagenesis, Insertional
- Proteins/genetics*
- Proviruses/genetics
- Retroviridae/genetics
- Sequence Alignment
- Zebrafish/genetics*
- Zebrafish Proteins
- PubMed
- 10801422 Full text @ Curr. Biol.
Citation
Kawakami, K., Amsterdam, A., Shimoda, N., Becker, T., Mugg, J., Shima, A., and Hopkins, N. (2000) Proviral insertions in the zebrafish hagoromo gene, encoding an F-box/WD40-repeat protein, cause stripe pattern anomalies. Current biology : CB. 10(8):463-466.
Abstract
The zebrafish, Danio rerio, has three types of pigment cells (melanophores, xanthophores and iridophores) and, in adult fish, these cells are organized into a stripe pattern. The mechanisms underlying formation of the stripe pattern are largely unknown. We report here the identification and characterization of a novel dominant zebrafish mutation, hagoromo (hag), which was generated by insertional mutagenesis using a pseudotyped retrovirus. The hag mutation caused disorganized stripe patterns. Two hag mutant alleles were isolated independently and proviruses were located within the fifth intron of a novel gene, which we named hag, encoding an F-box/WD40-repeat protein. The hag gene was mapped to linkage group (LG)13, close to fgf8 and pax2.1. Amino acid sequence similarity, conserved exon-intron boundaries and conserved synteny indicated that zebrafish hag is an ortholog of mouse Dactylin, the gene mutated in the Dactylaplasia (Dac) mouse [1]. The Dac mutation is dominant and causes defects in digit formation in fore- and hindlimbs. This study revealed that the hag locus is important for pattern formation in fish but is involved in distinct morphogenetic events in different vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping