ZFIN ID: ZDB-PUB-000510-5
Proviral insertions in the zebrafish hagoromo gene, encoding an F-box/WD40-repeat protein, cause stripe pattern anomalies
Kawakami, K., Amsterdam, A., Shimoda, N., Becker, T., Mugg, J., Shima, A., and Hopkins, N.
Date: 2000
Source: Current biology : CB   10(8): 463-466 (Journal)
Registered Authors: Amsterdam, Adam, Becker, Thomas S., Hopkins, Nancy, Kawakami, Koichi, Mugg, Jennifer, Shima, Akihiro, Shimoda, Nobuyoshi
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Body Patterning/genetics*
  • DNA, Complementary/genetics
  • F-Box Proteins
  • Mice
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Proteins/genetics*
  • Proviruses/genetics
  • Retroviridae/genetics
  • Sequence Alignment
  • Zebrafish/genetics*
  • Zebrafish Proteins
PubMed: 10801422 Full text @ Curr. Biol.
The zebrafish, Danio rerio, has three types of pigment cells (melanophores, xanthophores and iridophores) and, in adult fish, these cells are organized into a stripe pattern. The mechanisms underlying formation of the stripe pattern are largely unknown. We report here the identification and characterization of a novel dominant zebrafish mutation, hagoromo (hag), which was generated by insertional mutagenesis using a pseudotyped retrovirus. The hag mutation caused disorganized stripe patterns. Two hag mutant alleles were isolated independently and proviruses were located within the fifth intron of a novel gene, which we named hag, encoding an F-box/WD40-repeat protein. The hag gene was mapped to linkage group (LG)13, close to fgf8 and pax2.1. Amino acid sequence similarity, conserved exon-intron boundaries and conserved synteny indicated that zebrafish hag is an ortholog of mouse Dactylin, the gene mutated in the Dactylaplasia (Dac) mouse [1]. The Dac mutation is dominant and causes defects in digit formation in fore- and hindlimbs. This study revealed that the hag locus is important for pattern formation in fish but is involved in distinct morphogenetic events in different vertebrates.