Figure 3

Figure 3

Loss of Pla2g6 selectively reduces neuronal precursors without affecting neural progenitors. Whole-mount in situ hybridization and qRT-PCR were used to assess neural progenitor and neuronal precursor populations in control and Pla2g6-deficient zebrafish embryos. (A) sox2 at 8 hpf. Representative in situ hybridization images are shown on the left, with corresponding qRT-PCR measurements of sox2 mRNA expression on the right. sox2 expression levels remained comparable across all experimental groups. (B) neurog1 at 10 hpf. Representative images are shown on the left, and qRT-PCR quantification of neurog1 expression is shown on the right. qRT-PCR analysis demonstrated a significant reduction in neurog1 expression, with statistical evaluation performed across independent experiments. Both CRISPR-mediated Pla2g6 deficiency and D331Y-mediated deficiency resulted in a significant reduction in neurog1 expression. Relative mRNA expression was normalized to gapdh. Data are presented as mean ± SEM from three independent biological replicates with each analyzed in three technical repeats. Statistical significance was determined using one-way ANOVA with Tukey’s pairwise comparison; *, p < 0.05; **, p < 0.01; ns, not significant.