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Fig. 6 Loss of Tulp3 results in the upregulation of genes related to fibrosis during zebrafish embryogenesis. (A) qPCR analysis reveals unaltered expression of col1α1 while genes related to liver-fibrosis (acta2, hand2), inflammatory/damage (tgfβ, sdf1a) and liver function (gc, serpina1) were significantly upregulated in MZtulp3 embryos compared to the respective control at 4dpf. (B) WISH analyses indicate upregulated expression of gc and serpina1 in the liver (black arrow) in MZtulp3 embryos compared to the respective control at 4dpf (for each condition a lateral and dorsal view is shown). (C, D) Heatmaps of ECM organization (C) and Tgfβ signalling pathway (D) indicate an increase in related biological processes (GOBP) in MZtulp3 mutant compared to control embryos at 2dpf. Shown are respective up- or downregulated BPs.