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Figure 4
OCTA sequential imaging workflow. Sample preparation and selection are performed as first step in order to anaesthetize the larvae. Then, phytagel is used to fix the selected larvae in space for imaging over a glass dish filled with E3 medium. Next OCT depth resolved images and fluorescence GFP microscopy maps are recorded and OCTA images are calculated. Before any topological analysis can take place, images need to be co‐registered and a merged color‐coded image is created. The total imaging and analysis session take about 7 min per larvae.
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