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Fig. 5 Elevated levels of G4 structures in RMS tissue sections of pax3a:EGFP zebrafish. (A) Schematic illustration of 2 month-old pax3a:EGFP zebrafish with RMS tumor. (B) Tissue section of the tumor, showing DAPI (left) and pax3a:EGFP (right). NT: neural tube, AC: abdominal cavity, GI: gastrointestinal tract. Dashed lines indicates where the tumor is located. Scale bar = 200 μm. (C) Zoom of the boxed region from (B), showing DAPI in blue, pax3a:EGFP expression in green, BG4 staining for G4 DNA in red, and a merged image of all channels. Scale bar = 50 μm. (D) Zoom of the boxed region from (C). Scale bar = 10 μm.