Fig. 4

Fig. 4

The impact of IS20 on the expression of selected genes and proteins associated with oxidative stress and iron regulation in the K562 (A) and U-251 cells (B,C). The HO-1 expression was determined in kinetic experiments, while Ndrg1, calreticulin, and IDH-1 gene expression after 24-hour incubation. The levels of HO-1 and HIF-1α proteins were determined after 24-h treatment with IS20 in U-251 cells. The results of gene and protein expression are presented as the mean ± SD of several independent experiments (n = 5). Immunoblots shown are representative of five independent experiments. The displayed blots were cropped, and the full-length blots are shown in Fig. S6. The statistical analysis was performed using a t-test or one-way ANOVA with Bonferroni’s posthoc test: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 compared to the untreated controls.