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Fig. 2 Upregulation of Reelin and Dab-1 levels in the spinal cord after SCI. qPCR (A) and western blot (B, C) analysis of Reelin caudal to lesion site in 4–6 dpf larvae. Twenty-four larvae per group were pooled for qPCR and western blot analysis in three independent experiments. GAPDH served as the loading control for qPCR analysis and β-actin served as the loading control for western blot analysis. Data represent means ± SEM, *p < 0.05, one-way ANOVA with Tukey's post hoc test. Similarly, Dab-1 levels were determined after SCI by qPCR (D) and western blot analysis (E, F). Twenty-four larvae per group were pooled for qPCR and western blot analysis in three independent experiments. GAPDH served as the loading control for qPCR analysis and β-actin served as the loading control for western blot analysis. Data represent means ± SEM, *p < 0.05, **p < 0.01, one-way ANOVA with Tukey's post hoc test.