Bone geometrical parameters. (A) Representative 3-dimensional (3D) models of WT and untreated and treated Chi/+ fish. Scale bar: 100 μm. (B) Micro-CT (μCT) analysis of bone volume (BV), vertebral thickness (V.Th), and BMD in WT, Chi/+ (i), and Chi/+ untreated and treated with 4PBA, ALN, and 4PBA + ALN (ii). (C) Representative 3D models of WT and untreated and treated p3h1−/− fish. Scale bar: 100 μm. (D) μCT analysis of BV, V.Th, and BMD in WT, p3h1−/− (i), and p3h1−/− untreated and treated with 4PBA, ALN, and 4PBA + ALN (ii). In the graphs, each dot represents a single value. *p < .05, **p < .01, ***p < .001. Abbreviations: ALN, alendronate; 4PBA, 4-phenylbutyrate.
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