Figure 5

Figure 5

Knockout of rps20 caused intracranial hemorrhage. (A) Representative images of O-dianisidine staining of rps20 knockout and control zebrafish; (B) Representative images of intracranial hemorrhage of rps20 knockout zebrafish with dual-labeled vasculature and erythrocytes. Empty arrowheads indicate sites of erythrocyte aggregation outside the cerebral blood vessels; (C) Intracranial hemorrhage incidence statistics in control versus rps20-KO zebrafish. 65 WT larvae and 52 rps20-KO larvae were analyzed; (D) Regional hemorrhage distribution analysis (forebrain/midbrain/hindbrain) in rps20-KO zebrafish. A total of 21 rps20-KO larvae with intracranial hemorrhage were analyzed. Scale bar, 200 μm (A). Scale bar, 100 μm (B).