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Fig. 3 Lactobacillus reuteri E9 suppresses PTZ-induced neuronal hyperactivity in zebrafish brains. Zebrafish in (A) normal (control group), (B) PTZ-induced model (model group), (C) GABA-treated (positive group), and E9-ttreated with different concentrations (expriment groups): (D) 1 × 104 CFU/mL, (E) 1 × 105 CFU/mL and (F) 1 × 106 CFU/mL. The left part show fluorescence images of brain activity in Tg(Elavl3:H2B-GCaMP6f) zebrafish (blue: baseline activity; yellow: PTZ-induced hyperactivity). The right part present quantification of brain discharge area (normalized to control). Data are mean ± SEM (n = 8 per group). E9 treatment (1% v/v, 7 days) significantly reduced PTZ-induced neuronal excitation. Imaging parameters: 20 × objective, 488 nm excitation.