|
Fig. 2 Dependency of her gene expression on Notch signaling in the neural plate. A. Effects of Notch signaling inhibitor on her expression. Embryos were incubated with 100 μM DAPT or DMSO from the shield stage (6 hpf) to the bud stage (10 hpf) and subjected to WISH analysis. B. Effects of NICD-mediated Notch activation on her expression. Fertilized eggs were microinjected with pCS2+NICD-HT (NICD) or pCS2+ egfp (egfp) (10 pg/embryo) together with egfp mRNA (50 pg/embryo). Morphologically normal embryos with EGFP fluorescence were subjected to WISH at the bud stage. The expression of her4.1 (top row) or her6 (bottom row) are shown. Dorsal views with anterior to the top. Numbers of embryos showing the indicated phenotype and total numbers of scored embryos are shown in the bottom right. The experiment was repeated three times for both DAPT treatment and NICD overexpression, yielding essentially identical results. See the legend to Fig. 1 for abbreviations. Scale bar, 200 μm.