FIGURE 3

FIGURE 3

Visualization imaging of intracellular β‐gal. (a) X‐Gal staining of endogenous β‐gal in LO₂ cells and DOX‐induced LO₂ senescent cells, HepG2 cells and XL413‐induced HepG2 senescent cells, and 4T1 cells and palbociclib‐induced 4T1 senescent cells. (b) Fluorescence imaging of β‐gal with QMOH‐Gal in LO₂ cells and DOX‐induced LO₂ senescent cells, HepG2 cells and XL413‐induced HepG2 senescent cells, and 4T1 cells and palbociclib‐induced 4T1 senescent cells. (c) Relative fluorescent intensities in LO₂ cells and DOX‐induced LO₂ senescent cells. (d) Relative fluorescent intensities in HepG2 cells and XL413‐induced HepG2 senescent cells. (e) Relative fluorescent intensities in 4T1 cells and palbociclib‐induced 4T1 senescent cells. λ_ex = 640 nm, λ_em = 680–770 nm. Scale bars: 50 μm.