FIGURE 1

FIGURE 1

Spectral properties of the probe QMOH‐Gal (10 μM) to β‐gal in PBS containing 5% DMSO (pH = 7.4, 10 mM). (a) The ultraviolet‐visible absorption spectrum and (b) the fluorescence spectrum of QMOH‐Gal (10 μM) for β‐gal (0–500 U/L, λ_ex = 640 nm, slit widths: 5/5 nm). (c) The linear relationship between the fluorescence intensity of QMOH‐Gal and β‐gal (0–400 U/L). (d) The reaction of QMOH‐Gal to a series of various analytes: (1) blank, (2) β‐gal (500 U/L), (3) esterase (500 U/L), (4) ALP (500 U/L), (5) AFU (100 U/L), (6) Cys (1 mM), (7) Hcy (1 mM), (8) GSH (1 mM), (9) BSA (100 μg/ml), 10. HClO (100 μM), (11) NaNO₂ (100 μM), (12) H₂O₂ (100 μM), (13) Cl⁻ (100 μM), (14) Na⁺ (100 μM), (15) K⁺ (100 μM). Fluorescence spectrum at 740 nm of QMOH‐Gal (e) with time change and (f) pH effect to β‐gal at 37°C.