Figure 1.

Figure 1. Sephin1 protects primary WT and SOD1^G93A rat motor neurons against glutamate injury.

(A) Schematic of the experiment on primary WT and SOD1^G93A rat motor neurons. (B) Representative pictures of primary WT rat motor neurons treated with glutamate 5 μM for 20 min in presence of DMSO (vehicle) or Sephin1 500 nM 24 h after glutamate intoxication. Motor neurons are labeled with the neuronal marker, MAP-2. Right panel: enlargement of motor neurons (C) Representative pictures of primary SOD1^G93A rat motor neurons treated with glutamate 5 μM for 20 min in presence of DMSO (vehicle) or Sephin1 50 nM 24 h after glutamate intoxication. Motor neurons are labeled with the neuronal marker, MAP-2. Right panel: enlargement of motor neurons (D) Cleaved caspase 3 level measured 24 h after glutamate intoxication in primary WT rat motor neurons culture treated with glutamate 5 μM for 20 min in presence of DMSO (vehicle) or Sephin1 50 nM. (E) Cell viability in glutamate intoxicated primary WT rat motor neurons treated for 24 h with DMSO (Vehicle), Sephin1, or riluzole. (F) Total neurite length of primary WT rat motor neurons treated for 24 h with DMSO (Vehicle), Sephin1 or riluzole 24 h after glutamate intoxication. (G) Cleaved caspase 3 level measured 24 h after glutamate intoxication in primary SOD1^G93A rat motor neurons culture treated with glutamate 5 μM for 20 min in presence of DMSO (vehicle) or Sephin1 50 nM 24 h. (H) Cell viability in glutamate intoxicated primary SOD1^G93A rat motor neurons treated for 24 h with DMSO (Vehicle), Sephin1 or riluzole. (I) Total neurite length of primary WT rat motor neurons treated for 24 h with DMSO (Vehicle), Sephin1 or riluzole 24 h after glutamate intoxication. *P < 0.05, **P < 0.01 versus 0 nM Sephin1 Kruskal-Wallis test followed by Dunn’s multiple comparison test; mean ± SEM n = 4–6 replicates per condition.

Source data are available for this figure.