FIGURE 2

FIGURE 2

Aβ‐injected zebrafish larvae exhibit alterations in oligodendroglial lineage mRNA levels and regional distribution in the developing spinal cord. Zebrafish larvae were injected with Aβ or its vehicle at 24 hpf, and FISH assays were performed for sox10, myrf and mbp at 48 hpf, 72 hpf and 5 dpf. (A) Representative confocal images of spinal cord cross‐sections depicting mRNA expression fluorescence. Dorsal and ventral regions were defined based on the DAPI (blue) pattern, and are delineated by the dotted white line. (B–D) RNAscope analysis representing the total area occupied by each mRNA in the developing spinal cord. (E–G) Quantification of the regional distribution of each mRNA in the ventral and dorsal regions, expressed relative to the total occupied area. Scale bar = 20 μm. Data are represented as means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001 compared to vehicle‐injected zebrafish larvae. Statistical significance was drawn by two‐tailed nested t‐test and two‐way ANOVA, followed by Sidak's post hoc test. n = 8–11 larvae per condition and time point.