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Fig. 3 Lack of Crhr1 inhibits thermal stress-mediated metabolic rate changes in zebrafish. (A) Metabolic rate of WT and crhr1−/− fish at 28°C and 33°C after 60 min of acclimation in the resting intermittent-flow respirometer. MO2 was calculated as the mean of the three lowest consecutive measurements from each individual. (B) Whole-body cortisol concentration of WT zebrafish exposed for 4 days to 325.4 μM metyrapone and sampled immediately after 90 min exposure to the resting intermittent-flow respirometer at 33°C. (C) Metabolic rate of untreated and metyrapone-treated WT fish at 33°C after 60 min of acclimation in the resting intermittent-flow respirometer. MO2 was calculated as in (A). (A) Different letters denote significant changes between treatment groups (Two-way ANOVA, Holm–Sidak post hoc test, p < 0.05). (B, C) Asterisks indicate significant difference (Student's t-test; *p < 0.05). Crhr1, corticotropin-releasing hormone receptor 1; KO, crhr1-/-; MO2, metabolic rate; WT, wildtype.