Fig. 5

Fig. 5 Guanosine and inosine increase MFN2 expression.

a Heatmap of Z-Score values based on mRNA relative expression of 7 genes, involved in mitochondrial fusion and fission. b–d PCA biplot of 7 genes in CTRL, TMZ-treated and TMZ + GUA + INO-treated cells for U-251 MG S (b), U-251 MG R (c) and T98-G (d). Key-coloured arrows represent the contribution of each variable to the PCA; small circles are the single sample, while large circles are the mean points of the confidence ellipses. e mRNA expression levels of MFN2 in CTRL, TMZ-treated and TMZ + GUA + INO-treated cells for U-251 MG S, U-251 MG R, T98-G. Data are shown via scattered dot plots as mean ± SD of n ≥ 3 independent experiments. ***p-value < 0.001 and ****p-value < 0.0001 between groups. ###p-value < 0.001 and ####p-value < 0.0001 vs CTRL U-251 MG S. CTRL control, GUA guanosine, INO inosine, PCs principal components, PCA principal components analysis, TMZ temozolomide.