ZFIN Image: Reed et al., 2025, Figure 7

Image

Figure Caption

Figure 7 Characterization of GCaMP‐positive postsynaptic chain neurons (ChNs) in Tg(elavl3:GCaMP6s) zebrafish gills

A–C, confocal imaging of immunohistochemical localization of GCaMP in a ChN containing synaptic vesicle protein‐2 (SV2, magenta). B and C, GCaMP and SV2 labelling shown separately. Scale bar = 50 µm in (A) to (C). D–F, co‐labelling of GCaMP‐positive ChNs and NECs (green, arrows) with nerve fibres labelled with zn‐12 (magenta). E and F, GCaMP and zn‐12 labelling shown separately. Scale bar = 50 µm in (D) to (F). G–I, images from (D) to (F), cropped and titled back 90°. Rotation demonstrates neural connection (arrowhead) between nerve fibres located below the efferent filament artery (eFA) where ChNs are located, projecting to GCaMP‐positive NECs. [Colour figure can be viewed at wileyonlinelibrary.com]

Acknowledgments

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