Figure 4

Figure 4

Expression of neuronal markers in 3D neuronal epithelial stem cell (NESC) organoids derived from induced pluripotent stem cells (iPSCs) carrying the leucine-rich repeat kinase 2 (LRRK2) G2019S mutation. (A) Three-dimensional NESC organoids generated from both gene-corrected (GC) and (B) mutated (MUT T413) iPSC lines were stained with antibodies against TH, GFAP, and MAP2 neuronal markers and visualized using confocal microscopy. Representative micrographs are shown. (C) Through a semi-quantitative analysis of the images, we observed reductions in the expression levels of all examined neuronal markers: tyrosine hydroxylase (TH), glial fibrillary acidic protein (GFAP), and microtubule-associated protein 2 (MAP2) in the NESC 3D colonies derived from the LRRK2-G2019S-mutated T413 cell line, compared to the gene-corrected GC counterpart. In blue nuclei, in purple the MAP2 marker, in red the TH marker and in green the GFAP marker (* p < 0.05).