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Fig. 4 Therapeutic effect of exosomes on larval survival, neurological function, and cerebral cell viability. (A) Survival curve of zebrafish larvae (6 dpf) treated with vehicle, control exosomes (Exo_Control), or soy-derived exosomes (Exo_Soy) posthypoxia. Data represent n = 60 larvae per group. (B) Behavioral assessment of neurological function, evaluated immediately after hypoxia (0 h) and 2 days posthypoxia (2 d). Data are presented as mean ± SD from three independent experiments with n = 5 larvae per experiment. (C) Left: Representative fluorescence images of cranial motor neurons (green: GFP-labeled) in zebrafish larvae, acquired before and 2 days posthypoxia. Scale bar: 50 μm. Right: Quantification of relative cerebral cell viability at 2 days posthypoxia in the Vehicle, Exo_Control, and Exo_Soy groups. Data are presented as mean ± SD with individual data points (n = 5 per group).